FastQCFastQC Report
Fri 10 Feb 2017
ERR1631181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785296
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT118111.5040188667712557No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG92141.1733155396181822No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT57250.72902446975408Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC51050.6500733481387909No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC22300.2839693567775718No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC18610.23698070536460136Illumina PCR Primer Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17460.2223365457101526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.1931755669199894No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT14100.17955013141541534No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC13080.16656139850451296No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12730.16210448034881114No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC11890.15140787677512682No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG11790.15013447158778345No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT11740.14949776899411177No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11010.14020191112650515No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10970.13969254905156783No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10620.13523563089586602No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC9400.11970008761027688No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT9270.1180446608667305No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA8870.11295104011735703No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8620.10976752714899858No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA8560.10900348403659256No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA8230.10480124691835944No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG8130.10352784173101608No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC8050.10250911758114137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16250.031.7630771
TTATACA17500.029.62
GATTACG502.7189344E-729.61
TATACAC18150.029.3553713
TTACGGG702.0736479E-1029.071433
GGTATCA3250.029.030771
CCGTCTT8950.024.39106237
GCCGTCT9050.024.32596636
CGTCTTC3200.024.28125237
GTATCAA10300.024.2475721
CGAACTA802.7230271E-823.12500229
ATTACGG652.6810485E-622.769232
GTCTATC502.7010456E-422.21
GCGAACT909.468749E-820.55555728
ATCGTAC450.003824744420.55555725
CGAATTA555.141099E-420.18181815
TGCCGTC11300.019.31858435
TACACAT28000.018.96255
ATACACA28550.018.53244
TCGCCAT1800.018.513
Produced by FastQC (version 0.11.5)