##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631181.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785296
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486470069884476	31.0	31.0	34.0	28.0	34.0
2	31.487549917483342	31.0	31.0	34.0	28.0	34.0
3	30.956803549234937	31.0	31.0	34.0	27.0	34.0
4	35.10315091379556	37.0	35.0	37.0	32.0	37.0
5	35.305183013793524	37.0	35.0	37.0	33.0	37.0
6	35.49394623173937	37.0	35.0	37.0	33.0	37.0
7	35.50652238136957	37.0	35.0	37.0	33.0	37.0
8	35.52311612436584	37.0	35.0	37.0	33.0	37.0
9	37.29379749801349	39.0	37.0	39.0	34.0	39.0
10	37.13238702349178	39.0	37.0	39.0	33.0	39.0
11	37.180208481897274	39.0	37.0	39.0	33.0	39.0
12	37.128769279354536	39.0	37.0	39.0	33.0	39.0
13	37.182626678348036	39.0	37.0	39.0	33.0	39.0
14	38.33987566471751	40.0	38.0	41.0	34.0	41.0
15	38.349579012245066	40.0	38.0	41.0	34.0	41.0
16	38.34143176585644	40.0	38.0	41.0	34.0	41.0
17	38.323498400603086	40.0	38.0	41.0	34.0	41.0
18	38.35037489048715	40.0	38.0	41.0	34.0	41.0
19	38.394763248507566	40.0	38.0	41.0	34.0	41.0
20	38.38871075365213	40.0	38.0	41.0	34.0	41.0
21	38.34851189869807	40.0	38.0	41.0	34.0	41.0
22	38.28712485483181	40.0	38.0	41.0	34.0	41.0
23	38.29681546830749	40.0	38.0	41.0	34.0	41.0
24	38.25242201666633	40.0	38.0	41.0	34.0	41.0
25	38.22110516289399	40.0	38.0	41.0	34.0	41.0
26	38.144784896395755	40.0	38.0	41.0	33.0	41.0
27	38.07515000713107	40.0	37.0	41.0	33.0	41.0
28	38.071630315193254	40.0	37.0	41.0	33.0	41.0
29	38.01304858295471	40.0	37.0	41.0	33.0	41.0
30	37.93773685336485	40.0	37.0	41.0	33.0	41.0
31	37.92105779222102	40.0	37.0	41.0	33.0	41.0
32	37.83093890711273	40.0	37.0	41.0	33.0	41.0
33	37.82303997473564	40.0	37.0	41.0	33.0	41.0
34	37.75596080968195	40.0	37.0	41.0	33.0	41.0
35	37.73400603084697	40.0	37.0	41.0	33.0	41.0
36	37.71729768138383	40.0	37.0	41.0	33.0	41.0
37	37.66768326847456	40.0	37.0	41.0	33.0	41.0
38	37.61724878262464	40.0	37.0	41.0	32.0	41.0
39	37.552100099834966	40.0	37.0	41.0	32.0	41.0
40	37.492429606161245	40.0	37.0	41.0	32.0	41.0
41	37.45071157881869	40.0	37.0	41.0	32.0	41.0
42	37.40433288849045	39.0	37.0	41.0	32.0	41.0
43	36.765816965831995	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	11.0
12	13.0
13	6.0
14	5.0
15	3.0
16	9.0
17	6.0
18	15.0
19	33.0
20	52.0
21	129.0
22	224.0
23	470.0
24	818.0
25	1523.0
26	2496.0
27	3948.0
28	5949.0
29	8711.0
30	12274.0
31	16722.0
32	21921.0
33	28504.0
34	38073.0
35	50995.0
36	71465.0
37	106176.0
38	187644.0
39	227090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.43353334284142	22.46796112548644	14.773792302520322	24.324713229151808
2	13.75252134227094	22.51902467349891	41.45570587396345	22.2727481102667
3	14.700698844766812	28.38152747499032	34.88277031845317	22.035003361789695
4	12.023364438377376	19.442095719321124	37.987204824677576	30.54733501762393
5	12.203933293942667	39.01535217293861	35.99814592204723	12.782568611071493
6	27.347013100792566	40.10105743566757	18.290937429962714	14.260992033577146
7	23.852534585684886	33.75275055520466	23.178903241580244	19.215811617530203
8	23.64764369104134	36.73748497381879	22.155722173549847	17.459149161590023
9	23.836234999286894	16.02733236894114	19.78082149915446	40.35561113261751
10	14.044767832766244	29.62500764043112	34.16431511175404	22.165909415048592
11	30.930120616939345	23.49177889611051	25.982177420997942	19.5959230659522
12	20.284198569711293	26.577749027118436	29.735793891729994	23.402258511440273
13	27.842367718669138	21.53188097227033	27.81193333469163	22.8138179743689
14	21.104526191397895	22.54742160917667	26.90106660418492	29.44698559524052
15	23.411427028789145	29.08431979788513	23.79561337381064	23.708639799515087
16	24.045582812086145	28.4378119842709	23.820444774963835	23.69616042867912
17	21.12846620891995	30.472331452089403	25.34547482732626	23.05372751166439
18	22.824641918461317	27.09602493836719	26.635943644180028	23.44338949899146
19	23.58397343167417	27.925393736883926	27.301934557160614	21.18869827428129
20	22.79942849575192	27.00828732095923	26.79053503392351	23.401749149365337
21	23.190363888266337	26.945126423667	25.983960188260223	23.880549499806442
22	21.37716224200811	30.74293005439987	26.152941056620687	21.72696664697133
23	22.147827061388316	26.875471159919318	26.591756484179214	24.38494529451315
24	23.917605590758136	28.041400949450907	27.02713371773191	21.013859742059047
25	22.830881603879302	28.3109808276115	26.11728571137507	22.740851857134125
26	23.156873331839204	26.38380941708604	27.358091725922456	23.101225525152298
27	22.21328008801777	29.57177930360017	26.478168741468185	21.736771866913877
28	20.468332959801145	26.70203337340315	27.54604633157434	25.28358733522137
29	22.836484586703612	27.222219392432915	28.176381899309305	21.764914121554167
30	21.53048022656425	28.645631710845336	26.89673702654795	22.92715103604246
31	22.15865100548074	26.3195024551252	27.290983272549457	24.230863266844604
32	24.28294553900695	27.09869908926061	26.982946557731097	21.635408814001345
33	21.179529756932418	26.178154479330086	28.085970131007926	24.55634563272957
34	21.263065137222142	28.095138648356798	28.164029868992074	22.477766345428986
35	23.166551211263016	26.54374910861637	28.953795766182434	21.335903913938186
36	22.26039607994947	26.892534789429718	25.765953220187036	25.08111591043377
37	22.75243984433895	27.128114749088244	27.60538701330454	22.51405839326827
38	22.388627982314947	26.228708665267618	28.159318269798906	23.22334508261853
39	23.315666958700923	26.56934455288197	26.953912919459665	23.161075568957436
40	21.538502679244516	25.720237973961414	30.43081844298201	22.310440903812065
41	22.17851612640329	27.026369674619506	26.846055500091687	23.949058698885516
42	23.11230415028219	25.737301603471813	28.560822925368267	22.58957132087773
43	22.486298160184184	25.58602106721542	28.8910168904464	23.03666388215399
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	13.5
2	19.0
3	60.5
4	102.0
5	102.0
6	135.0
7	168.0
8	170.0
9	172.0
10	289.5
11	407.0
12	407.0
13	617.0
14	827.0
15	2029.5
16	3232.0
17	3750.0
18	4268.0
19	4268.0
20	4761.5
21	5255.0
22	6897.5
23	8540.0
24	11004.5
25	13469.0
26	13469.0
27	16774.5
28	20080.0
29	25722.0
30	31364.0
31	33777.0
32	36190.0
33	36190.0
34	39844.0
35	43498.0
36	45741.0
37	47984.0
38	52282.0
39	56580.0
40	56580.0
41	59431.0
42	62282.0
43	58293.0
44	54304.0
45	54841.5
46	55379.0
47	55379.0
48	55084.5
49	54790.0
50	55931.5
51	57073.0
52	66614.0
53	76155.0
54	76155.0
55	57864.0
56	39573.0
57	40739.0
58	41905.0
59	34396.5
60	26888.0
61	26888.0
62	21491.0
63	16094.0
64	13444.5
65	10795.0
66	9050.0
67	7305.0
68	7305.0
69	5869.5
70	4434.0
71	3669.0
72	2904.0
73	2236.0
74	1568.0
75	1568.0
76	1219.5
77	871.0
78	662.0
79	453.0
80	324.0
81	195.0
82	195.0
83	141.0
84	87.0
85	66.5
86	46.0
87	32.0
88	18.0
89	18.0
90	14.5
91	11.0
92	7.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	785296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.96820402669828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.51382581351795	42.88095334900345
2	10.66691642552974	11.086809782753376
3	2.7632440983228914	4.308024992316419
4	1.1585579194465088	2.4083269733817305
5	0.6091637640399404	1.5828573387649556
6	0.40654179624163844	1.267634820747952
7	0.2717952113751037	0.9887296298754684
8	0.20662462586861488	0.8590328571264303
9	0.14751384032771375	0.6899426415821179
>10	1.018144584052414	10.26077275659982
>50	0.12276455129893232	4.505450110513785
>100	0.10434964154041688	10.484372821586923
>500	0.0063837427765179875	2.2969973655565368
>1k	0.0031918713882589937	2.315498899719724
>5k	7.365857049828447E-4	2.557550005289147
>10k+	2.455285683276149E-4	1.507045655182105
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	11811	1.5040188667712557	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	9214	1.1733155396181822	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	5725	0.72902446975408	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	5105	0.6500733481387909	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	2230	0.2839693567775718	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	1861	0.23698070536460136	Illumina PCR Primer Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1746	0.2223365457101526	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1517	0.1931755669199894	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1410	0.17955013141541534	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1308	0.16656139850451296	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1273	0.16210448034881114	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1189	0.15140787677512682	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1179	0.15013447158778345	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1174	0.14949776899411177	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1101	0.14020191112650515	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1097	0.13969254905156783	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1062	0.13523563089586602	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	940	0.11970008761027688	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	927	0.1180446608667305	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	887	0.11295104011735703	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	862	0.10976752714899858	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	856	0.10900348403659256	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	823	0.10480124691835944	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	813	0.10352784173101608	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	805	0.10250911758114137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2734051873433713E-4	0.0
4	0.0	0.0	0.0	1.2734051873433713E-4	0.0
5	0.0	0.0	0.0	1.2734051873433713E-4	0.0
6	0.0	0.0	0.0	1.2734051873433713E-4	0.0
7	0.0	0.0	0.0	1.2734051873433713E-4	0.0
8	0.0	0.0	0.0	1.2734051873433713E-4	0.0
9	0.0	0.0	0.0	2.5468103746867425E-4	0.0
10	0.0	0.0	0.0	2.5468103746867425E-4	0.0
11	0.0	0.0	0.0	2.5468103746867425E-4	0.0
12	0.0	0.0	0.0	2.5468103746867425E-4	0.0
13	0.0	0.0	0.0	2.5468103746867425E-4	0.0
14	0.0	0.0	0.0	2.5468103746867425E-4	0.0
15	0.0	0.0	0.0	2.5468103746867425E-4	0.0
16	0.0	0.0	0.0	2.5468103746867425E-4	0.0
17	0.0	0.0	0.0	2.5468103746867425E-4	0.0
18	0.0	0.0	0.0	2.5468103746867425E-4	0.0
19	0.0	0.0	0.0	3.8202155620301135E-4	0.0
20	0.0	0.0	0.0	3.8202155620301135E-4	0.0
21	0.0	0.0	0.0	7.640431124060227E-4	0.0
22	0.0	0.0	0.0	0.001273405187343371	0.0
23	0.0	0.0	0.0	0.0016554267435463824	0.0
24	0.0	0.0	0.0	0.0024194698559524054	0.0
25	0.0	0.0	0.0	0.002546810374686742	0.0
26	0.0	0.0	0.0	0.0028014914121554167	0.0
27	0.0	0.0	0.0	0.00394755608076445	0.0
28	0.0	0.0	0.0	0.00751309060532589	0.0
29	0.0	0.0	0.0	0.0157902243230578	0.0
30	0.0	0.0	0.0	0.03170778916484994	0.0
31	0.0	0.0	0.0	0.0716927120474318	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1625	0.0	31.763077	1
TTATACA	1750	0.0	29.6	2
GATTACG	50	2.7189344E-7	29.6	1
TATACAC	1815	0.0	29.355371	3
TTACGGG	70	2.0736479E-10	29.07143	3
GGTATCA	325	0.0	29.03077	1
CCGTCTT	895	0.0	24.391062	37
GCCGTCT	905	0.0	24.325966	36
CGTCTTC	320	0.0	24.281252	37
GTATCAA	1030	0.0	24.247572	1
CGAACTA	80	2.7230271E-8	23.125002	29
ATTACGG	65	2.6810485E-6	22.76923	2
GTCTATC	50	2.7010456E-4	22.2	1
GCGAACT	90	9.468749E-8	20.555557	28
ATCGTAC	45	0.0038247444	20.555557	25
CGAATTA	55	5.141099E-4	20.181818	15
TGCCGTC	1130	0.0	19.318584	35
TACACAT	2800	0.0	18.9625	5
ATACACA	2855	0.0	18.5324	4
TCGCCAT	180	0.0	18.5	13
>>END_MODULE