FastQCFastQC Report
Fri 10 Feb 2017
ERR1631180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352802
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT75212.131790636107505RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG65271.8500462015521455No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT64451.826803702926854No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC38061.0787920703397373No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC24760.7018100804417209No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC23730.6726152346075136RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.5728425575818731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16850.4776050022392163No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG9070.25708471040413605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6840.19387645194755132No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA6490.18395587326602456No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT6260.17743663584673558No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5440.15419413722144434No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5010.14200599769842573No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3540.1003395672360134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG200.001840207837.07
GGACCGT200.001840207837.06
CTTATAC8750.032.7714271
GATTACG303.5944674E-430.8333341
CGTCTTC3350.030.37313737
TATACAC9700.029.7525773
TTATACA10050.029.2686582
GGTATCA1850.028.01
CCGTCTT11950.026.78242737
GCCGTCT12300.026.02032536
TCGCCGA451.3206738E-424.6666681
GCCGAGC451.3206738E-424.6666683
GTATCAA5750.024.1304361
GGTATAT400.001928549723.1251
AAAGCGA400.001928549723.12520
CGCCGAG502.6969615E-422.22
TCACGGT705.0854032E-621.1428571
ACGGTAC705.0854032E-621.1428573
ATTGAGC806.932023E-720.81256
TGCCGTC15400.020.78246935
Produced by FastQC (version 0.11.5)