##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	352802
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49591272158321	31.0	31.0	34.0	28.0	34.0
2	31.50410145067205	31.0	31.0	34.0	30.0	34.0
3	31.006612774304	31.0	31.0	34.0	27.0	34.0
4	35.128746435677805	37.0	35.0	37.0	32.0	37.0
5	35.31087692246643	37.0	35.0	37.0	33.0	37.0
6	35.487922404068	37.0	35.0	37.0	33.0	37.0
7	35.48455224176734	37.0	35.0	37.0	33.0	37.0
8	35.515773720103624	37.0	35.0	37.0	33.0	37.0
9	37.272954801843525	39.0	37.0	39.0	34.0	39.0
10	37.127643834218624	39.0	37.0	39.0	33.0	39.0
11	37.17481193417271	39.0	37.0	39.0	33.0	39.0
12	37.10568250746878	39.0	37.0	39.0	33.0	39.0
13	37.19406636016802	39.0	37.0	39.0	33.0	39.0
14	38.291741543415284	40.0	38.0	41.0	33.0	41.0
15	38.304677978015995	40.0	38.0	41.0	33.0	41.0
16	38.3128865482622	40.0	38.0	41.0	34.0	41.0
17	38.30258615313972	40.0	38.0	41.0	33.0	41.0
18	38.37215491975669	40.0	38.0	41.0	34.0	41.0
19	38.38360893645728	40.0	38.0	41.0	34.0	41.0
20	38.36122244204965	40.0	38.0	41.0	34.0	41.0
21	38.32492729633052	40.0	38.0	41.0	34.0	41.0
22	38.24286426947693	40.0	38.0	41.0	34.0	41.0
23	38.23955929955046	40.0	38.0	41.0	34.0	41.0
24	38.21052885187726	40.0	38.0	41.0	34.0	41.0
25	38.16385111195515	40.0	37.0	41.0	33.0	41.0
26	38.010459124381384	40.0	37.0	41.0	33.0	41.0
27	37.95094132119432	40.0	37.0	41.0	33.0	41.0
28	37.92525836021338	40.0	37.0	41.0	33.0	41.0
29	37.89309300967682	40.0	37.0	41.0	33.0	41.0
30	37.78616617819627	40.0	37.0	41.0	33.0	41.0
31	37.70094273841985	40.0	37.0	41.0	33.0	41.0
32	37.60580438886401	40.0	37.0	41.0	32.0	41.0
33	37.48553012738023	39.0	37.0	41.0	32.0	41.0
34	37.4016502174024	39.0	36.0	41.0	32.0	41.0
35	37.39747790545405	39.0	36.0	41.0	32.0	41.0
36	37.33636714077585	39.0	36.0	41.0	32.0	41.0
37	37.28248706073095	39.0	36.0	41.0	31.0	41.0
38	37.25791520456233	39.0	36.0	41.0	31.0	41.0
39	37.11267509821373	39.0	36.0	41.0	31.0	41.0
40	36.95855182226858	39.0	36.0	41.0	31.0	41.0
41	36.93644026961299	39.0	36.0	41.0	31.0	41.0
42	36.84562162346018	39.0	36.0	41.0	30.0	41.0
43	36.204990334521916	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	8.0
11	11.0
12	15.0
13	7.0
14	10.0
15	11.0
16	10.0
17	5.0
18	8.0
19	25.0
20	26.0
21	70.0
22	131.0
23	302.0
24	502.0
25	850.0
26	1299.0
27	2141.0
28	3067.0
29	4415.0
30	6191.0
31	8009.0
32	10390.0
33	13408.0
34	17905.0
35	23763.0
36	32816.0
37	48477.0
38	82450.0
39	96470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.974002414952295	23.883368008117866	16.411755035402294	23.73087454152754
2	12.699474492774984	22.595960340360882	41.34216926207902	23.36239590478512
3	13.71959342634112	29.436057618720984	35.67043270729758	21.17391624764032
4	10.5861077885046	19.50527491340752	38.074330644384105	31.834286653703774
5	11.734060464509838	38.66361301806679	35.68205395661022	13.920272560813146
6	26.40801356001383	40.66020033900035	18.07104268116394	14.860743419821883
7	22.69970124885913	34.611481794320895	23.026230010033956	19.662586946786014
8	25.035288915595714	34.72741084234217	22.938362027426148	17.298938214635974
9	22.59057488336234	15.203995442202709	20.29722053729854	41.90820913713641
10	14.07049846656198	28.775630523636487	33.81358382322095	23.340287186580575
11	30.024206211982925	23.526227175582907	25.32808770925335	21.121478903180822
12	19.07160390247221	27.961292736435734	29.466386244975933	23.50071711611612
13	28.622853611940975	20.71586895765897	26.919348529770236	23.741928900629816
14	21.327260049546204	23.36324624010068	27.281307929093373	28.028185781259747
15	23.211886553931098	29.998129262305767	23.097941621646136	23.692042562116995
16	24.305134324635347	27.88561289335095	24.028775347078533	23.780477434935175
17	19.45312101405321	32.069829536113744	25.680976865210514	22.796072584622536
18	22.427593947880116	25.67247351205492	27.39100118480054	24.508931355264426
19	22.05344640903396	28.525065050651637	28.954767830114342	20.466720710200057
20	20.721254414657515	29.255503086717194	26.39043996349227	23.63280253513302
21	23.802869598244907	27.874275089143485	27.211863878322685	21.11099143428892
22	22.25412554350599	31.277033576907158	27.000980720063943	19.467860159522903
23	23.93155367599957	26.215270888487023	27.15432452196983	22.698850913543573
24	22.711039053066592	27.824388750630664	27.541227090549373	21.92334510575337
25	23.184108933622824	29.58429940873351	26.391857189018204	20.839734468625462
26	21.880828339975398	29.669049495184268	28.49275230866038	19.957369856179955
27	21.713879173020562	31.59817688108344	26.975187215491975	19.71275673040402
28	20.52992896865664	28.3351568301767	29.230843362565974	21.90407083860069
29	21.999308393943345	26.53981553392554	29.004370723521976	22.456505348609134
30	22.93581102147947	28.02450099489232	29.304822535019643	19.73486544860857
31	21.160310882591368	28.241336500359974	26.476323830363775	24.122028786684883
32	25.59565988854938	26.241631283269367	28.315599117918833	19.847109710262416
33	21.296647978186066	28.050861389674665	30.284975708754487	20.36751492338479
34	20.33690285202465	29.347055855692428	27.508347458347743	22.807693833935183
35	22.1092850947557	26.028764009274326	32.468069908900745	19.393880987069235
36	22.306846333070673	28.21752711152431	26.521108156983235	22.95451839842178
37	20.808555507054947	26.878815879728574	29.72999019279936	22.582638420417116
38	21.07074222935244	25.151501408722172	29.397225639310438	24.380530722614953
39	23.801168927613787	25.953651056399906	27.806531709003917	22.438648306982387
40	19.78758623817325	25.230015702858825	33.72288138956128	21.259516669406636
41	21.577258632320678	26.420485144642036	27.17133122828102	24.830924994756266
42	21.336897183122545	25.447418098536858	31.91705262441823	21.29863209392237
43	21.785590784632742	24.94657059767235	31.011162068242243	22.256676549452667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	9.5
2	13.0
3	62.5
4	112.0
5	112.0
6	160.5
7	209.0
8	204.5
9	200.0
10	288.5
11	377.0
12	377.0
13	604.0
14	831.0
15	2083.0
16	3335.0
17	3635.5
18	3936.0
19	3936.0
20	4226.5
21	4517.0
22	5138.5
23	5760.0
24	7078.5
25	8397.0
26	8397.0
27	9786.5
28	11176.0
29	12603.5
30	14031.0
31	15535.5
32	17040.0
33	17040.0
34	18193.0
35	19346.0
36	20507.5
37	21669.0
38	22166.5
39	22664.0
40	22664.0
41	22796.5
42	22929.0
43	22857.0
44	22785.0
45	22866.5
46	22948.0
47	22948.0
48	23391.0
49	23834.0
50	28884.5
51	33935.0
52	26666.0
53	19397.0
54	19397.0
55	24098.0
56	28799.0
57	23693.0
58	18587.0
59	13828.0
60	9069.0
61	9069.0
62	7729.5
63	6390.0
64	5304.5
65	4219.0
66	3475.5
67	2732.0
68	2732.0
69	2201.0
70	1670.0
71	1334.5
72	999.0
73	728.5
74	458.0
75	458.0
76	332.0
77	206.0
78	161.0
79	116.0
80	89.5
81	63.0
82	63.0
83	47.5
84	32.0
85	20.0
86	8.0
87	5.5
88	3.0
89	3.0
90	2.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	352802.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.86798953488059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17259178425431	50.99111833598394
2	8.949298967830043	10.715530739011333
3	2.3407082663372774	4.204004939798659
4	0.9827285427259117	2.3533592844617335
5	0.5375316935512343	1.609047090209597
6	0.36141046310650676	1.298215069383402
7	0.254668284581668	1.0672534737340904
8	0.1896257289996826	0.9082008927437681
9	0.13862030768169897	0.7469027216648894
>10	0.9744312923640603	10.77513680079965
>50	0.07236949178292926	2.946178864159377
>100	0.019393131784966624	1.9740304557914188
>500	0.0028380192856048716	1.1075072604504717
>1k	0.0023650160713373928	3.5003572606566813
>5k	0.0014190096428024358	5.8031568111509895
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7521	2.131790636107505	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	6527	1.8500462015521455	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	6445	1.826803702926854	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	3806	1.0787920703397373	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2476	0.7018100804417209	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2373	0.6726152346075136	RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.5728425575818731	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1685	0.4776050022392163	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	907	0.25708471040413605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	684	0.19387645194755132	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	649	0.18395587326602456	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	626	0.17743663584673558	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	544	0.15419413722144434	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	501	0.14200599769842573	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	354	0.1003395672360134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.834451051864785E-4	0.0
12	0.0	0.0	0.0	2.834451051864785E-4	0.0
13	0.0	0.0	0.0	2.834451051864785E-4	0.0
14	0.0	0.0	0.0	2.834451051864785E-4	0.0
15	2.834451051864785E-4	0.0	0.0	2.834451051864785E-4	0.0
16	2.834451051864785E-4	0.0	0.0	2.834451051864785E-4	0.0
17	2.834451051864785E-4	0.0	0.0	2.834451051864785E-4	0.0
18	2.834451051864785E-4	0.0	0.0	2.834451051864785E-4	0.0
19	2.834451051864785E-4	0.0	0.0	2.834451051864785E-4	0.0
20	2.834451051864785E-4	0.0	0.0	8.503353155594356E-4	0.0
21	2.834451051864785E-4	0.0	0.0	0.001133780420745914	0.0
22	2.834451051864785E-4	0.0	0.0	0.0017006706311188712	0.0
23	2.834451051864785E-4	0.0	0.0	0.0017006706311188712	0.0
24	2.834451051864785E-4	0.0	0.0	0.002267560841491828	0.0
25	2.834451051864785E-4	0.0	0.0	0.0025510059466783068	0.0
26	2.834451051864785E-4	0.0	0.0	0.0031178961570512637	0.0
27	2.834451051864785E-4	0.0	0.0	0.0039682314726107	0.0
28	2.834451051864785E-4	0.0	0.0	0.008503353155594357	0.0
29	2.834451051864785E-4	0.0	0.0	0.018423931837121106	0.0
30	2.834451051864785E-4	0.0	0.0	0.03486374793793686	0.0
31	2.834451051864785E-4	0.0	0.0	0.06887716056031429	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACG	20	0.0018402078	37.0	7
GGACCGT	20	0.0018402078	37.0	6
CTTATAC	875	0.0	32.771427	1
GATTACG	30	3.5944674E-4	30.833334	1
CGTCTTC	335	0.0	30.373137	37
TATACAC	970	0.0	29.752577	3
TTATACA	1005	0.0	29.268658	2
GGTATCA	185	0.0	28.0	1
CCGTCTT	1195	0.0	26.782427	37
GCCGTCT	1230	0.0	26.020325	36
TCGCCGA	45	1.3206738E-4	24.666668	1
GCCGAGC	45	1.3206738E-4	24.666668	3
GTATCAA	575	0.0	24.130436	1
GGTATAT	40	0.0019285497	23.125	1
AAAGCGA	40	0.0019285497	23.125	20
CGCCGAG	50	2.6969615E-4	22.2	2
TCACGGT	70	5.0854032E-6	21.142857	1
ACGGTAC	70	5.0854032E-6	21.142857	3
ATTGAGC	80	6.932023E-7	20.8125	6
TGCCGTC	1540	0.0	20.782469	35
>>END_MODULE