FastQCFastQC Report
Fri 10 Feb 2017
ERR1631179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631553
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT152152.409140642194717No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG139742.2126409026637512No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT119701.8953278663865105RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC79001.2508847238474048No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC42440.6719942744314412RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC36010.5701817583005702RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27050.4283092630388899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26300.41643377515426255No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA21540.3410640120464949No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG19620.31066276306184915No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA12120.19190788421557653No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT10200.16150663523093076No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT9800.15517304169246285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9480.15010616686168857No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7630.12081329674627467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18600.034.3145141
TTATACA19650.032.763362
CGTCTTC5600.032.04464337
TATACAC20850.030.8776973
GACGAAC250.005494490729.610
GCAACGC454.004498E-628.7777793
GATTACG604.31628E-827.7499981
GGTATCA4150.026.7469881
CCGTCTT18150.025.5840237
GCCGTCT18550.024.83288436
ACCTAAG451.3222899E-424.6666681
TTAAGGT451.3222899E-424.6666684
GTATCAA12050.024.2572611
AGCAACG551.9008998E-523.5454542
CCGTCCT400.001930229123.12532
CGTCCTA400.001930229123.12533
GCTTTAT4800.023.1249981
CTTCGTC603.7228652E-521.58333221
TCGCCCG705.095917E-621.14285910
TCACCCG705.095917E-621.1428595
Produced by FastQC (version 0.11.5)