FastQCFastQC Report
Fri 10 Feb 2017
ERR1631178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098534
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT183031.6661295872499167No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG140651.280342711286132No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT106970.9737522916905621Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC79890.7272419424432925No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC42340.3854227543253099Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC39070.35565581038001554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27080.24651034924726956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.23312887903332988No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG19130.17414117360045298No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA12960.11797541086575382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23450.032.9765471
TTATACA24950.030.9939862
TATACAC25650.030.5087723
CGTCTTC5550.027.66666437
GATTACG706.5920176E-926.428571
GGTATCA5250.025.723811
CCGTCTT17100.025.42397737
GCCGTCT17200.025.27616336
GTATCAA14600.022.808221
AAGTACG450.003825702720.55555535
TGCCGTC21900.019.85159735
TACACAT42200.018.9822275
ATGCCGT23200.018.81896634
ATACACA43150.018.7786794
CGCGCTT609.236461E-418.512
ATGTACG609.236461E-418.511
TATGCCG24200.017.96487633
CGGTGTA1258.576535E-917.7634
ACGGTAC1156.404116E-817.6956523
AATCGTC752.0671618E-417.26666628
Produced by FastQC (version 0.11.5)