##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631178.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1098534
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.424932683012088	31.0	31.0	34.0	28.0	34.0
2	31.433557814323454	31.0	31.0	34.0	28.0	34.0
3	30.87697695292089	31.0	31.0	34.0	27.0	34.0
4	35.0234876662898	37.0	35.0	37.0	32.0	37.0
5	35.251390489506925	37.0	35.0	37.0	33.0	37.0
6	35.44728793100623	37.0	35.0	37.0	33.0	37.0
7	35.463588746456644	37.0	35.0	37.0	33.0	37.0
8	35.48046305348765	37.0	35.0	37.0	33.0	37.0
9	37.263917184174545	39.0	37.0	39.0	34.0	39.0
10	37.089100564934725	39.0	37.0	39.0	33.0	39.0
11	37.15038405729818	39.0	37.0	39.0	33.0	39.0
12	37.08095425357795	39.0	37.0	39.0	33.0	39.0
13	37.15850032861978	39.0	37.0	39.0	33.0	39.0
14	38.29125361618302	40.0	38.0	41.0	34.0	41.0
15	38.29544374593777	40.0	38.0	41.0	33.0	41.0
16	38.28239817793532	40.0	38.0	41.0	33.0	41.0
17	38.25757600584051	40.0	38.0	41.0	33.0	41.0
18	38.28399029979955	40.0	38.0	41.0	33.0	41.0
19	38.32179522891417	40.0	38.0	41.0	34.0	41.0
20	38.316984271765826	40.0	38.0	41.0	34.0	41.0
21	38.27300748087906	40.0	38.0	41.0	34.0	41.0
22	38.206892094373046	40.0	38.0	41.0	34.0	41.0
23	38.20460267957114	40.0	38.0	41.0	34.0	41.0
24	38.165262977750345	40.0	38.0	41.0	33.0	41.0
25	38.152445896076046	40.0	37.0	41.0	33.0	41.0
26	38.03699020694853	40.0	37.0	41.0	33.0	41.0
27	37.969585829842316	40.0	37.0	41.0	33.0	41.0
28	37.943128751590756	40.0	37.0	41.0	33.0	41.0
29	37.870754114119364	40.0	37.0	41.0	33.0	41.0
30	37.80764182082667	40.0	37.0	41.0	33.0	41.0
31	37.75996191287661	40.0	37.0	41.0	33.0	41.0
32	37.69056488010385	40.0	37.0	41.0	33.0	41.0
33	37.651125044832476	40.0	37.0	41.0	32.0	41.0
34	37.583358366695975	40.0	37.0	41.0	32.0	41.0
35	37.584401575190206	40.0	37.0	41.0	32.0	41.0
36	37.564827306209914	40.0	37.0	41.0	32.0	41.0
37	37.49032528806573	40.0	37.0	41.0	32.0	41.0
38	37.458287135400454	39.0	37.0	41.0	32.0	41.0
39	37.36931128212691	39.0	36.0	41.0	31.0	41.0
40	37.28519827333519	39.0	36.0	41.0	31.0	41.0
41	37.223025413869756	39.0	36.0	41.0	31.0	41.0
42	37.16634532932071	39.0	36.0	41.0	31.0	41.0
43	36.510577733597685	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	14.0
11	8.0
12	11.0
13	11.0
14	7.0
15	8.0
16	12.0
17	11.0
18	24.0
19	32.0
20	81.0
21	157.0
22	415.0
23	754.0
24	1464.0
25	2391.0
26	3851.0
27	6023.0
28	9143.0
29	13251.0
30	18100.0
31	24124.0
32	31885.0
33	41554.0
34	55058.0
35	74551.0
36	103274.0
37	152411.0
38	263983.0
39	295917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.94784685772129	21.88516695887428	15.0144647320884	25.15252145131603
2	14.020503689462501	21.43165345815423	41.1158871732691	23.431955679114164
3	14.528635435953735	28.096171807153898	33.84028168449952	23.534911072392845
4	11.971864320995072	19.157531765061435	37.97014930807786	30.900454605865633
5	12.766559796965774	37.99554679236146	35.76366320933171	13.474230201341058
6	28.11073667269288	39.81679219760153	17.796445080443572	14.276026049262017
7	25.064586075624423	32.55147314511886	23.06828919268771	19.31565158656901
8	24.89654393946842	35.218846207764166	21.92522033910648	17.959389513660934
9	24.28864286403516	15.071267707690431	19.605128289156276	41.03496113911813
10	14.869180198337057	28.47722510181751	33.5187622777265	23.134832422118933
11	31.579177339982195	23.080487267576608	25.368263522112194	19.972071870329003
12	20.81783540609576	26.014306339175665	29.34501799671198	23.82284025801659
13	28.748495722481053	20.596267389083998	26.835400633935773	23.819836254499176
14	21.794500670894116	21.91666348060233	26.56549546941651	29.723340379087038
15	23.818834919993375	28.5396719628159	22.771530057330953	24.869963059859774
16	24.506569664662177	26.941086939503013	23.967942730948703	24.58440066488611
17	21.287188198089453	29.982777046500154	25.267401828254748	23.462632927155646
18	23.85388162769655	25.82468999594004	25.833428915263433	24.487999461099974
19	23.977682984777896	26.910682782690387	25.779903034407674	23.331731198124047
20	23.959021750806077	26.068469432898755	25.608128651457307	24.364380164837865
21	25.144055623221494	25.806028761968225	25.4908814838685	23.559034130941782
22	22.458567509062078	30.118230296012687	25.765702290507164	21.65749990441807
23	24.518039496274124	25.03791416560616	25.84171268253873	24.60233365558098
24	23.297230672878584	27.182954737859728	25.664203383782386	23.855611205479303
25	23.27520131375087	26.95465047053619	26.379611372975255	23.390536842737685
26	24.226196003036772	26.00747905845427	26.67209207907994	23.09423285942902
27	23.28485053717045	28.835156672437996	25.734842981646448	22.14514980874511
28	23.689298647106053	25.297532893838515	27.24649396377354	23.766674495281894
29	22.039099381539398	26.863255939279075	26.226771315225566	24.870873363955965
30	25.02243899597099	25.55569513551697	26.888107241104965	22.53375862740707
31	24.407346518177864	25.288702944105506	26.439236291275463	23.864714246441167
32	22.545046398199784	27.633009083014272	26.57833075717274	23.243613761613204
33	23.531360886417716	25.640171355643066	28.79483020097694	22.03363755696228
34	22.03363755696228	27.09957088264906	26.404280613981907	24.462510946406756
35	22.249743749396924	25.226256083107124	29.36094831839524	23.16305184910071
36	23.256995231827144	25.8195922930014	27.002259374766734	23.92115310040472
37	22.6481838522977	26.752562961182814	27.583306479362495	23.015946707156992
38	22.686052502699052	25.37481771160474	27.933591495575012	24.005538290121198
39	23.757571454320033	25.737846985163866	26.72407044297218	23.780511117543927
40	21.89936770277479	24.76227408528093	30.735598534046282	22.602759677898
41	22.8552780341801	25.85099778431983	26.31944027221734	24.974283909282736
42	23.097874075813767	25.18856949352501	28.94621377217273	22.767342658488495
43	23.302146314997987	24.499287231892687	29.115075182015303	23.083491271094022
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	19.5
2	29.0
3	86.0
4	143.0
5	143.0
6	185.0
7	227.0
8	246.5
9	266.0
10	408.5
11	551.0
12	551.0
13	864.5
14	1178.0
15	2771.5
16	4365.0
17	4947.0
18	5529.0
19	5529.0
20	5730.5
21	5932.0
22	7439.0
23	8946.0
24	12130.5
25	15315.0
26	15315.0
27	19280.0
28	23245.0
29	28448.5
30	33652.0
31	39449.5
32	45247.0
33	45247.0
34	51257.0
35	57267.0
36	62045.5
37	66824.0
38	70869.5
39	74915.0
40	74915.0
41	77777.5
42	80640.0
43	81206.5
44	81773.0
45	81427.5
46	81082.0
47	81082.0
48	79414.0
49	77746.0
50	74966.0
51	72186.0
52	71287.0
53	70388.0
54	70388.0
55	82162.0
56	93936.0
57	71538.0
58	49140.0
59	52662.0
60	56184.0
61	56184.0
62	47761.0
63	39338.0
64	28743.0
65	18148.0
66	14876.0
67	11604.0
68	11604.0
69	9533.5
70	7463.0
71	6255.5
72	5048.0
73	4939.5
74	4831.0
75	4831.0
76	4044.5
77	3258.0
78	2363.0
79	1468.0
80	937.0
81	406.0
82	406.0
83	278.5
84	151.0
85	110.5
86	70.0
87	44.5
88	19.0
89	19.0
90	14.5
91	10.0
92	6.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1098534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.88262026650881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57413481863765	35.70804979224259
2	14.149978514868922	13.55076096013454
3	4.70785641275356	6.762735026433811
4	2.0661088969095047	3.9572283095989285
5	1.10187930486024	2.638043416707378
6	0.6769364939470554	1.944809585052521
7	0.4552498797268163	1.5258989982133064
8	0.30167648639341843	1.1556048513048534
9	0.24293957586510811	1.0469325112970111
>10	1.4884286997881693	13.606953105946468
>50	0.15490142505075588	5.122616686255946
>100	0.07686532033329424	6.309984173142494
>500	7.610427164040848E-4	0.3155766299708248
>1k	0.0015220854328081696	1.703694733569398
>5k	1.902606791010212E-4	0.7278122681399882
>10k+	5.707820373030636E-4	3.9232989519900605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	18303	1.6661295872499167	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	14065	1.280342711286132	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	10697	0.9737522916905621	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	7989	0.7272419424432925	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	4234	0.3854227543253099	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	3907	0.35565581038001554	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2708	0.24651034924726956	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2561	0.23312887903332988	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	1913	0.17414117360045298	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1296	0.11797541086575382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.10304096186372E-5	0.0
5	0.0	0.0	0.0	9.10304096186372E-5	0.0
6	0.0	0.0	0.0	9.10304096186372E-5	9.10304096186372E-5
7	9.10304096186372E-5	0.0	0.0	9.10304096186372E-5	9.10304096186372E-5
8	9.10304096186372E-5	0.0	0.0	9.10304096186372E-5	9.10304096186372E-5
9	9.10304096186372E-5	0.0	0.0	1.820608192372744E-4	9.10304096186372E-5
10	1.820608192372744E-4	0.0	0.0	1.820608192372744E-4	9.10304096186372E-5
11	1.820608192372744E-4	0.0	0.0	1.820608192372744E-4	9.10304096186372E-5
12	1.820608192372744E-4	0.0	0.0	1.820608192372744E-4	9.10304096186372E-5
13	1.820608192372744E-4	0.0	0.0	1.820608192372744E-4	9.10304096186372E-5
14	1.820608192372744E-4	0.0	0.0	1.820608192372744E-4	9.10304096186372E-5
15	1.820608192372744E-4	0.0	0.0	2.730912288559116E-4	9.10304096186372E-5
16	2.730912288559116E-4	0.0	0.0	3.641216384745488E-4	9.10304096186372E-5
17	2.730912288559116E-4	0.0	0.0	3.641216384745488E-4	9.10304096186372E-5
18	3.641216384745488E-4	0.0	0.0	3.641216384745488E-4	1.820608192372744E-4
19	4.55152048093186E-4	0.0	0.0	3.641216384745488E-4	1.820608192372744E-4
20	4.55152048093186E-4	0.0	0.0	4.55152048093186E-4	1.820608192372744E-4
21	5.461824577118232E-4	0.0	0.0	7.282432769490976E-4	1.820608192372744E-4
22	5.461824577118232E-4	0.0	0.0	9.10304096186372E-4	1.820608192372744E-4
23	5.461824577118232E-4	0.0	0.0	9.10304096186372E-4	1.820608192372744E-4
24	5.461824577118232E-4	0.0	0.0	0.001274425734660921	1.820608192372744E-4
25	5.461824577118232E-4	0.0	0.0	0.001365456144279558	1.820608192372744E-4
26	5.461824577118232E-4	0.0	0.0	0.0015475169635168324	1.820608192372744E-4
27	5.461824577118232E-4	0.0	0.0	0.002548851469321842	1.820608192372744E-4
28	5.461824577118232E-4	0.0	0.0	0.008829949733007809	1.820608192372744E-4
29	7.282432769490976E-4	0.0	0.0	0.020026690116100185	1.820608192372744E-4
30	7.282432769490976E-4	0.0	0.0	0.03613907261859897	1.820608192372744E-4
31	7.282432769490976E-4	0.0	0.0	0.08129015578944303	1.820608192372744E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2345	0.0	32.976547	1
TTATACA	2495	0.0	30.993986	2
TATACAC	2565	0.0	30.508772	3
CGTCTTC	555	0.0	27.666664	37
GATTACG	70	6.5920176E-9	26.42857	1
GGTATCA	525	0.0	25.72381	1
CCGTCTT	1710	0.0	25.423977	37
GCCGTCT	1720	0.0	25.276163	36
GTATCAA	1460	0.0	22.80822	1
AAGTACG	45	0.0038257027	20.555555	35
TGCCGTC	2190	0.0	19.851597	35
TACACAT	4220	0.0	18.982227	5
ATGCCGT	2320	0.0	18.818966	34
ATACACA	4315	0.0	18.778679	4
CGCGCTT	60	9.236461E-4	18.5	12
ATGTACG	60	9.236461E-4	18.5	11
TATGCCG	2420	0.0	17.964876	33
CGGTGTA	125	8.576535E-9	17.76	34
ACGGTAC	115	6.404116E-8	17.695652	3
AATCGTC	75	2.0671618E-4	17.266666	28
>>END_MODULE