FastQCFastQC Report
Fri 10 Feb 2017
ERR1631177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367381
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG112333.0575887158018515No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT84992.3134021628772308RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT65961.7954113032519374No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC60941.658768417528397No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC29100.7920932220229135RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC28000.7621515538364804No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG14940.40666229336846493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14860.4044847175003607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.4039403235333346No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA8790.23926114850795224No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8170.22238493553014443RNA PCR Primer, Index 26 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT8100.22047955664555324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5410.14725856808054855No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4160.11323394514141995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7900.033.0189861
CGTCTTC3900.032.25641337
GCAACGC303.5947544E-430.8333323
TTTGGCG250.005491537529.59999822
CGCCGAG250.005491537529.5999982
GGTATCA2450.028.6938761
TTATACA9250.028.42
TCCTATA358.857864E-426.428572
TATACAC10300.026.4029123
CCGTCTT12400.025.51209637
GCCGTCT12450.025.26104436
GTATCAA5950.022.3865551
AGGACTG705.0863455E-621.1428575
TGCCGTC15250.020.62295235
CCTTATA450.003820929220.5555552
AGCAACG450.003820929220.5555552
TTAGCGT555.133944E-420.18181825
TTATGGG555.133944E-420.1818183
TACTAAG555.133944E-420.18181811
TCGCCAT656.885804E-519.92307713
Produced by FastQC (version 0.11.5)