##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367381
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.455268508714386	31.0	30.0	33.0	27.0	34.0
2	30.450401626649175	31.0	30.0	33.0	27.0	34.0
3	29.865801443188406	31.0	30.0	34.0	25.0	34.0
4	34.127170430697284	35.0	35.0	37.0	30.0	37.0
5	33.972404669811446	35.0	33.0	37.0	30.0	37.0
6	34.24949575508804	35.0	35.0	37.0	30.0	37.0
7	34.3020488266949	35.0	35.0	37.0	30.0	37.0
8	34.36653773602881	35.0	35.0	37.0	30.0	37.0
9	36.000702268217466	37.0	35.0	39.0	32.0	39.0
10	35.67173043788329	37.0	35.0	39.0	30.0	39.0
11	35.82134623184106	37.0	35.0	39.0	30.0	39.0
12	35.69005201684355	37.0	35.0	39.0	30.0	39.0
13	35.828129380670205	37.0	35.0	39.0	30.0	39.0
14	36.650109831482844	38.0	36.0	40.0	31.0	41.0
15	36.66094327142667	38.0	36.0	40.0	31.0	41.0
16	36.66162920782512	38.0	36.0	40.0	31.0	41.0
17	36.56741638789159	38.0	36.0	40.0	31.0	41.0
18	36.713703212740995	38.0	36.0	40.0	31.0	41.0
19	36.71079614895708	38.0	36.0	40.0	31.0	41.0
20	36.747891153870235	38.0	36.0	40.0	31.0	41.0
21	36.702148450790865	38.0	36.0	40.0	31.0	41.0
22	36.495197084225914	38.0	35.0	40.0	30.0	41.0
23	36.52763207678132	38.0	35.0	40.0	30.0	41.0
24	36.368478500521256	38.0	35.0	40.0	30.0	41.0
25	36.379415375318814	38.0	35.0	40.0	30.0	41.0
26	36.21454294043514	38.0	35.0	40.0	30.0	41.0
27	36.098815670924736	38.0	35.0	40.0	30.0	41.0
28	36.121326361461264	38.0	35.0	40.0	30.0	41.0
29	36.02662630892724	38.0	34.0	40.0	29.0	41.0
30	35.942038374330735	38.0	34.0	40.0	29.0	41.0
31	35.89495646209249	38.0	34.0	40.0	29.0	41.0
32	35.73081623709446	38.0	34.0	40.0	29.0	41.0
33	35.64472577514896	38.0	34.0	40.0	29.0	41.0
34	35.53498956124568	38.0	34.0	40.0	28.0	41.0
35	35.54227355252449	38.0	34.0	40.0	28.0	41.0
36	35.54785903462618	38.0	34.0	40.0	28.0	41.0
37	35.48321497301167	38.0	34.0	40.0	28.0	41.0
38	35.421649459280694	38.0	34.0	40.0	27.0	41.0
39	35.300589850863275	38.0	34.0	40.0	27.0	41.0
40	35.106780154662324	38.0	33.0	40.0	27.0	40.0
41	35.106042500837006	38.0	33.0	40.0	27.0	40.0
42	35.02485975050425	38.0	33.0	40.0	26.0	40.0
43	34.12291599184498	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	9.0
10	14.0
11	7.0
12	11.0
13	13.0
14	9.0
15	13.0
16	15.0
17	17.0
18	33.0
19	55.0
20	106.0
21	243.0
22	420.0
23	746.0
24	1197.0
25	1901.0
26	3020.0
27	4500.0
28	6380.0
29	8838.0
30	11726.0
31	15196.0
32	19678.0
33	25283.0
34	32533.0
35	42658.0
36	54126.0
37	63400.0
38	56163.0
39	19067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.77615881060806	25.66735895432807	16.596938872723413	22.959543362340458
2	13.87170267379097	23.27148110544639	40.58103168100691	22.27578453975573
3	15.554152228884998	30.530974655738863	34.59024827086866	19.324624844507472
4	11.470108688255516	20.12488397603578	35.82166742428161	32.5833399114271
5	11.264055571736154	40.309379091460904	34.773164643789414	13.65340069301352
6	25.82958835650183	40.0690291550189	17.97534439723339	16.126038091245874
7	21.784741181498227	35.658349234173784	22.32069704203538	20.236212542292606
8	24.287320247917012	34.76935388602024	23.835201058301873	17.10812480776088
9	22.145946578620016	15.06800841633073	19.15722369964696	43.62882130540229
10	13.617742888173312	28.580411071884505	34.240475147054426	23.561370892887766
11	29.717922265985447	23.178389737084935	24.676017540373618	22.427670456556
12	18.656109052999476	28.251597115800763	28.444584777111498	24.647709054088264
13	29.914992882048878	20.57264801391471	26.18861617775552	23.32374292628089
14	21.06886311485896	24.515149123117418	26.170378979860143	28.24560878216348
15	24.184157591165576	29.61122104844834	22.40970545564414	23.79491590474194
16	24.250845852126265	27.83704110991042	22.759478579458385	25.15263445850493
17	19.2854284788816	32.448058010621125	24.374695479624695	23.89181803087258
18	22.245026280618756	25.243003857031255	26.302394516863963	26.20957534548602
19	22.48782598991238	28.45737803533661	28.20559582558706	20.849200149163945
20	20.854644088834206	26.13036602328373	25.55249182728557	27.46249806059649
21	24.20702213778067	25.50131879438512	26.54247225632246	23.749186811511755
22	19.711688955063	31.97906260802818	27.861266641443077	20.447981795465743
23	24.64471488726962	25.92621828564896	26.07048268691086	23.358584140170557
24	22.15084612432325	30.78493444135652	27.660113070626952	19.404106363693277
25	22.859374872407663	26.80269257256091	25.499141218517018	24.83879133651441
26	21.135551375819652	26.77356749532502	28.809873128985984	23.281007999869345
27	20.235123754358554	29.258181560831943	27.941292554595908	22.565402130213595
28	20.336925426192426	25.436535912309022	28.437235458556646	25.789303202941905
29	19.54102144640033	27.18458494042969	31.292581815608315	21.981811797561658
30	23.601656046447694	25.283289010591186	28.619335240526866	22.495719702434258
31	20.50078801026727	28.11822059387938	25.896004420479013	25.48498697537434
32	24.56115041333112	26.69653574899083	29.51513551326824	19.227178324409806
33	21.151883194830432	25.594410162746577	30.17548539527085	23.07822124715214
34	19.949316921669872	32.03132442886268	26.54628301409164	21.47307563537581
35	22.02318573905564	25.245725826866387	33.33814214670873	19.39294628736924
36	22.023730133022664	25.878311616550665	25.38372969750749	26.714228552919177
37	21.312751612086636	26.56696998483863	29.615304003201036	22.5049743998737
38	21.041371219524148	25.105544380357177	29.192037693838273	24.661046706280402
39	25.10010044068692	25.984468440120743	26.991052885151927	21.92437823404041
40	19.58511735772944	24.553528897792752	34.69096115476849	21.170392589709323
41	21.791546106086052	27.393360026784187	26.63202506389824	24.18306880323152
42	21.32418388539418	24.545907382254388	32.495148088768886	21.63476064358255
43	22.553153265955505	24.440294952651335	30.347513888851086	22.659037892542074
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	11.5
2	16.0
3	51.5
4	87.0
5	87.0
6	120.0
7	153.0
8	162.5
9	172.0
10	267.0
11	362.0
12	362.0
13	539.0
14	716.0
15	1739.5
16	2763.0
17	3096.5
18	3430.0
19	3430.0
20	3548.5
21	3667.0
22	4411.5
23	5156.0
24	6455.0
25	7754.0
26	7754.0
27	9489.0
28	11224.0
29	13431.5
30	15639.0
31	16812.5
32	17986.0
33	17986.0
34	19164.5
35	20343.0
36	21191.0
37	22039.0
38	22936.0
39	23833.0
40	23833.0
41	24309.0
42	24785.0
43	23542.0
44	22299.0
45	22165.5
46	22032.0
47	22032.0
48	21660.5
49	21289.0
50	22237.0
51	23185.0
52	31309.5
53	39434.0
54	39434.0
55	28398.0
56	17362.0
57	22946.0
58	28530.0
59	22435.0
60	16340.0
61	16340.0
62	11425.5
63	6511.0
64	5304.5
65	4098.0
66	3347.0
67	2596.0
68	2596.0
69	2026.5
70	1457.0
71	1227.0
72	997.0
73	781.5
74	566.0
75	566.0
76	424.0
77	282.0
78	218.0
79	154.0
80	107.0
81	60.0
82	60.0
83	47.5
84	35.0
85	23.0
86	11.0
87	9.0
88	7.0
89	7.0
90	5.5
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	367381.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.35856215990596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7372493268914	52.079569679094504
2	7.468120337299368	8.865937705184848
3	1.7992198792342324	3.2039731512259104
4	0.7676329833788367	1.8226236063954695
5	0.4376660264410039	1.2989613017888688
6	0.2646473442501393	0.9425451500475555
7	0.19879126709091066	0.82599746491236
8	0.1629722637914578	0.7739039400484671
9	0.12973835712678522	0.6930974102430925
>10	0.9143963837728611	10.678864596113485
>50	0.0838335134954674	3.3569204134646653
>100	0.029776931022976547	3.0461095562274267
>500	0.0018324265244908644	0.8285570271223862
>1k	0.0022905331556135806	2.7665701325707768
>5k	0.0013743198933681483	5.7618296185415945
>10k+	4.581066311227161E-4	3.0545392470186292
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	11233	3.0575887158018515	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	8499	2.3134021628772308	RNA PCR Primer, Index 26 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	6596	1.7954113032519374	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	6094	1.658768417528397	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	2910	0.7920932220229135	RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	2800	0.7621515538364804	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	1494	0.40666229336846493	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1486	0.4044847175003607	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1484	0.4039403235333346	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	879	0.23926114850795224	No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	817	0.22238493553014443	RNA PCR Primer, Index 26 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT	810	0.22047955664555324	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	541	0.14725856808054855	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	416	0.11323394514141995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.721969835130287E-4	0.0
8	0.0	0.0	0.0	2.721969835130287E-4	0.0
9	0.0	0.0	0.0	2.721969835130287E-4	0.0
10	0.0	0.0	0.0	5.443939670260574E-4	0.0
11	0.0	0.0	0.0	5.443939670260574E-4	0.0
12	0.0	0.0	0.0	5.443939670260574E-4	0.0
13	0.0	0.0	0.0	5.443939670260574E-4	0.0
14	0.0	0.0	0.0	5.443939670260574E-4	0.0
15	0.0	0.0	0.0	0.0010887879340521149	0.0
16	0.0	0.0	0.0	0.0010887879340521149	0.0
17	0.0	0.0	0.0	0.0010887879340521149	0.0
18	0.0	0.0	0.0	0.0010887879340521149	0.0
19	0.0	0.0	0.0	0.0010887879340521149	0.0
20	0.0	0.0	0.0	0.0010887879340521149	0.0
21	0.0	0.0	0.0	0.0013609849175651436	0.0
22	0.0	0.0	0.0	0.001905378884591201	0.0
23	0.0	0.0	0.0	0.0024497728516172583	0.0
24	0.0	0.0	0.0	0.002994166818643316	0.0
25	0.0	0.0	0.0	0.002994166818643316	0.0
26	0.0	0.0	0.0	0.0032663638021563444	0.0
27	0.0	0.0	0.0	0.0035385607856693734	0.0
28	0.0	0.0	0.0	0.005988333637286632	0.0
29	0.0	0.0	0.0	0.013337652192138406	0.0
30	0.0	0.0	0.0	0.025586516450224698	0.0
31	0.0	0.0	0.0	0.056889169554223	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	790	0.0	33.018986	1
CGTCTTC	390	0.0	32.256413	37
GCAACGC	30	3.5947544E-4	30.833332	3
TTTGGCG	25	0.0054915375	29.599998	22
CGCCGAG	25	0.0054915375	29.599998	2
GGTATCA	245	0.0	28.693876	1
TTATACA	925	0.0	28.4	2
TCCTATA	35	8.857864E-4	26.42857	2
TATACAC	1030	0.0	26.402912	3
CCGTCTT	1240	0.0	25.512096	37
GCCGTCT	1245	0.0	25.261044	36
GTATCAA	595	0.0	22.386555	1
AGGACTG	70	5.0863455E-6	21.142857	5
TGCCGTC	1525	0.0	20.622952	35
CCTTATA	45	0.0038209292	20.555555	2
AGCAACG	45	0.0038209292	20.555555	2
TTAGCGT	55	5.133944E-4	20.181818	25
TTATGGG	55	5.133944E-4	20.181818	3
TACTAAG	55	5.133944E-4	20.181818	11
TCGCCAT	65	6.885804E-5	19.923077	13
>>END_MODULE