FastQCFastQC Report
Fri 10 Feb 2017
ERR1631176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279391
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG102153.656166447738116No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT77162.7617210289522567No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT74242.657207998826018TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC58322.087397231836387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53191.9037835864433714No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49291.7641942653843539No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC26880.9620925512990754No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC26480.947775697857125RNA PCR Primer, Index 12 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25910.9273741817023454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18760.6714604264274798No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18530.6632282356983582No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16470.589496440472313No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15420.5519147001871928No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG14860.5318711053684622No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13250.47424577026461123No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11840.4237788618817356No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11440.4094620084397851No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11310.4048090310711512No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10410.37259611082676247No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10040.35935302139295827No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA8660.3099598770182289No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8630.3088861130100826No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT8240.29492718090418085No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8050.28812667551925436No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7980.28562122616691304No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7520.26915684470866996No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7480.2677251593644749No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7180.25698751928301194No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT6710.24016521648872008No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6690.23944937381662257No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6510.23300678976774483No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6420.22978549774330595No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6410.22942757640725722No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6370.22799589106306217No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6300.2254904417107208No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6250.223700835030477No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6140.2197637003339406No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6120.2190478576618431No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6110.2186899363257943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.21725825098159926No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5900.21117358826877028No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5440.19470920681052717No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG5290.18934038676979575No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5200.18611909474535687No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5200.18611909474535687No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5140.18397156672906428No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4780.1710863986313088No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4740.16965471328711376No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4630.16571757859057737No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4440.15891707320565088No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.1546220171730657No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4260.15247448915677314No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4190.1499690398044318No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4100.14674774777999292No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4050.14495814109974908No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4030.14424229842765157No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4010.14352645575555406No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3980.14245269174740777No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3970.142094770411359No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3930.14066308506716393No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3870.13851555705087135No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3790.13565218636248125No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3770.13493634369038374No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3770.13493634369038374No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3760.13457842235433498No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3690.13207297300199364No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3550.12706207429731092No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3520.12598831028916463No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3480.1245566249449696No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3450.1234828609368233No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3440.12312493960077454No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3410.12205117559262826No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3390.12133533292053073No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3350.11990364757633568No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3270.11704027688794556No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3240.11596651287979927No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3230.11560859154375051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3230.11560859154375051No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3220.11525067020770176No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3140.11238729951931165No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3070.1098818501669703No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3060.10952392883092155No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2930.10487095146228761No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT2880.10308134478204381No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2870.10272342344599503No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2860.10236550210994627No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG2800.1002179740936537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGAC308.289462E-637.016
ATTACTT355.610982E-737.02
ATTACGG650.037.02
TTACTTT355.610982E-737.03
ACAGACA308.289462E-637.017
GATTACG650.037.01
ACTCAGA251.231235E-436.9999961
CTTATAC8300.036.3313261
TTACGGG700.034.3571433
TTATACA8900.033.8820232
AGCAACG507.2905095E-933.32
TAGCAAC507.2905095E-933.31
CGTCTTC3550.032.3098637
TTTACAC352.3818886E-531.7142852
GGCAAAT352.3818886E-531.71428527
CGGGGAC352.3818886E-531.71428516
TCCAGGC352.3818886E-531.71428523
TGATACA303.592568E-430.8333342
CCTAGTT303.592568E-430.8333342
CAGCACC303.592568E-430.8333344
Produced by FastQC (version 0.11.5)