FastQCFastQC Report
Fri 10 Feb 2017
ERR1631175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245135
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG155096.326717930936016No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT135835.541028412915332RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT120394.9111713953535805No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC87173.5559997552369103No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC48821.9915556734044506RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC43391.770045077202358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39611.6158443306749342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38721.5795378056989007No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG21230.8660533991474085No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19520.7962959185754789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14540.5931425541028412No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA14070.5739694454076325No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12650.5160421808391294No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12170.4964611336610439No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT12160.49605319517816715No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11920.4862626715891244No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT10480.4275195300548677No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9470.38631774328431273No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9370.38223835845554494No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8350.3406286332021131No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7960.3247190323699186No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7830.31941583209252045No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7770.31696820119525976No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6750.275358475941828No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5840.23823607400004077No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5540.22599791951373732No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5480.22355028861647663No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5460.22273441165072305No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5060.20641687233565179No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4930.20111367205825362No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4870.19866604116099293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.19213902543496442No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4590.18724376364044304No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4460.18194056336304484No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4450.18153262488016808No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT4360.17786117853427702No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4260.17378179370550922No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4220.1721500397740021No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4220.1721500397740021No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4160.1697024088767414No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4130.16847859342811106No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4080.16643890101372713No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3860.15746425439043793No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3800.15501662349317724No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3740.15256899259591655No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3740.15256899259591655No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3720.15175311563016297No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3710.15134517714728618No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3580.14604197686988804No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3570.14563403838701122No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.14563403838701122No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3530.14400228445550411No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3390.13829114569522916No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3380.13788320721235237No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3150.1285006221061864No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3140.12809268362330958No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3080.1256450527260489No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3080.1256450527260489No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3050.12442123727741856No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2980.1215656678972811No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2950.12034185244865073No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2900.11830216003426684No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2900.11830216003426684No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2880.11748628306851328No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2810.11463071368837578No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2810.11463071368837578No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2810.11463071368837578No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA2770.11299895975686866No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2760.11259102127399187No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2760.11259102127399187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2730.11136720582536153No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2730.11136720582536153No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2720.11095926734248475No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2630.10728782099659372No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2600.10606400554796337No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA2570.10484019009933303No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2560.10443225161645624No Hit
CTGATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT2550.10402431313357946No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2550.10402431313357946No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2490.10157668223631877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTT200.00183911237.03
GATACAC403.812238E-837.03
TGATACA403.812238E-837.02
ATTACGG355.607235E-737.02
TCGCCGA308.285313E-637.01
TGCTTGA452.6029738E-937.025
CTTATAA200.00183911237.01
CAGACAC403.812238E-837.018
GATTACG355.607235E-737.01
CTTATAC14300.035.706291
CTGCTTG554.965841E-1033.63636424
GCTTGAA507.2814146E-933.30000326
TTACGGG401.5932765E-632.3753
TTATACA15850.032.331232
CGCCGAG352.3807052E-531.7142852
TTGAAAA601.2605597E-930.83333228
CTTGAAA601.2605597E-930.83333227
CGTCTTC5750.030.56521637
TATACAC17150.029.9883383
GTTTGTG250.00548801429.61
Produced by FastQC (version 0.11.5)