FastQCFastQC Report
Fri 10 Feb 2017
ERR1631174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402846
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT183924.565516351161486No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG149943.7220178430467223No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT112082.7822046141701793TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC82762.0543830644961103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62711.5566742626214485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60501.5018145891978574No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC43231.073114788281378RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC39420.9785377042343724TruSeq Adapter, Index 11 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA37720.9363379554469947No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA24880.617605735194094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21920.5441285255407774No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG19630.4872829815860155No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19530.4848006434220521No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA16720.4150469410146806No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16460.4085928617883757No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC15780.3917129622734246No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT15760.3912164946406319No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14980.37185425696171737No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA14890.3696201526141503No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14680.3644072424698272No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13600.33759799029902243No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT11960.2968876444100227No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11210.2782701081802972No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10940.271567795137596No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT10290.2554325970718339No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10080.25021968692751073No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9820.24376560770120592No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT9470.235077424127334No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9390.2330915535961633No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG9260.22986451398301086No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9240.2293680463502182No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9230.22911981253382185No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9020.22390690238949873No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8920.2214245642255353No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8830.21919045987796826No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG8010.19883528693346839No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7770.1928776753399562No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7700.19114003862518184No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7690.1908918048087855No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7560.18766476519563308No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7270.1804659845201392No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6810.16904722896590757No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6780.16830252751671856No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6720.1668131246183405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.16234491592320638No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6090.15117439418537107No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5910.14670618549023695No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5860.14546501640825527No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5660.14050034008032847No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG5530.13727330046717606No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5400.1340462608540236No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5390.1337980270376273No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5290.13131568887366388No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5270.1308192212408712No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5260.13057098742447487No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5250.13032275360807852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.12858511689330412No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5120.1270957139949261No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5000.12411690819817002No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4990.12386867438177367No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4940.12262750529979198No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4740.11766282897186517No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4740.11766282897186517No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4720.1171663613390725No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4690.11642165988988348No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4640.11518049080790178No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4550.11294638646033472No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4500.11170521737835303No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4480.11120874974556033No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4450.11046404829637131No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4350.10798171013240791No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4300.10674054105042621No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4240.10525113815204817No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4210.10450643670285917No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4170.1035135014372738No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4080.10127939708970674No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4040.10028646182412136No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4040.10028646182412136No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4040.10028646182412136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACTC200.001840516937.031
GTTGTAT251.2322026E-436.9999961
CTTATAC19750.035.3139271
ATTACGG750.034.5333332
GATTACG750.034.5333331
CGTCTTC5300.034.20754637
TTATACA20900.033.370812
TTATCAA401.5966525E-632.3752
TATACAC21900.032.015983
GGTATCA7450.031.7852341
ACTCAGA658.731149E-1131.3076921
GTTTGTG303.5953644E-430.8333341
TTACGGG850.030.4705873
AGCGTGC250.00549215929.5999988
AACCCAC250.00549215929.5999985
TCAGAGT702.055458E-1029.0714283
AGAGTTG453.999763E-628.77777728
GAGTTGT453.999763E-628.77777729
GTTGGTT453.999763E-628.77777734
AGTTGTT509.07196E-625.89999830
Produced by FastQC (version 0.11.5)