FastQCFastQC Report
Fri 10 Feb 2017
ERR1631173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218260
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG155587.1281957298634655No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT125525.750939246769907TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT105014.811234307706405No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC85103.899019518006048No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC45552.086960505818748RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC39911.8285531018051866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29501.351599010354623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27681.2682122239530835No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG19420.8897645010537891No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA14220.6515165399065336No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13510.618986529826812No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT12600.5772931366260423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10850.49711353431686983No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9950.45587831027215253No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9220.42243196188032617No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8910.4082287180427014No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8770.4018143498579676No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7360.3372124988545771No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6160.28223220012828737No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6040.2767341702556584No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5800.2657381105104004No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5550.2542838816090901No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5470.2506185283606708No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4600.21075781178411068No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4090.18739118482543757No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT3850.1763951250801796No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3820.17502061761202237No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3770.1727297718317603No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3760.17227160267570787No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3730.17089709520755064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.1704389260514982No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3570.163566388710712No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3530.16173371208650233No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3510.1608173737743975No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3430.15715202052597818No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3400.15577751305782095No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT3340.15302849812150646TruSeq Adapter, Index 11 (95% over 23bp)
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3260.14936314487308716No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3240.14844680656098233No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3180.14569779162466784No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3140.14386511500045818No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3100.14203243837624852No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3100.14203243837624852No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3010.1379089159717768No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2980.13653440850361953No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2930.13424356272335747No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2900.1328690552552002No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2840.13012004031888572No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.12828736369467605No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA2720.12462201044625676No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2720.12462201044625676No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2670.12233116466599468No Hit
CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT2590.11866581141757536No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2590.11866581141757536No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2590.11866581141757536No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2560.11729130394941811No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2510.11500045816915606No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2490.11408411985705123No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2470.1131677815449464No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2450.11225144323284157No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2430.11133510492073674No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2350.10766975167231742No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2350.10766975167231742No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2330.10675341336021259No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2240.10262989095574088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG251.2303509E-437.02
ATTACGG200.001838671437.02
GTATACA200.001838671437.02
TTACGGG200.001838671437.03
GATTACG200.001838671437.01
CTTATAC12100.035.7768631
CGTCTTC6000.034.22537
GATACAC554.947651E-1033.6363643
GTATTAA401.592216E-632.3752
TTTACAC401.592216E-632.3752
TGATACA601.2587407E-930.8333342
TAGCAAC303.5900142E-430.8333341
TTATACA14150.030.593642
AACTTTA502.7037277E-729.67
ATTACTT250.005486711429.62
CTATACA250.005486711429.61
GGTCCCT250.005486711429.616
AACTCTC250.005486711429.625
GCACTAG250.005486711429.630
CTGTGCA250.005486711429.69
Produced by FastQC (version 0.11.5)