FastQCFastQC Report
Fri 10 Feb 2017
ERR1631172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704114
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG41880.5947900482024218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32920.46753792709703257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31110.44183186245409123No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA22150.3145797413487021No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG18480.2624574997798652No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG18030.25606648923327757No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC16520.2346210982880613No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT16500.23433705337487964No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT14190.20152986590239647No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG13230.18789571006967623No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT11670.16574020684150578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11440.16247369033991654No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC11430.1623316678833257No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC10730.1523900959219672No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG10540.1496916692467413No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC10520.1494076243335596No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT9950.14131234430788195No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT9510.13506335621788518No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC9410.13364313165197683No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC9390.13335908673879512No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA9210.13080268252016009No Hit
AACTAGTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCAC8870.12597391899607166No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8710.12370155969061829No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA8040.11418605509903226No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC7870.11177167333698804No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT7770.11035144877107968No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG7620.10822111192221714No Hit
GGTCTGATATGGACAATACTAGTTAAGAAAGCTAACAGGATGT7250.1029662810283562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG352.3870038E-531.7142851
GGTATCA4050.030.1481481
GTATCAA14750.024.9593221
TTTACCG400.00193044823.12500230
CGCCGTT400.00193044823.12500225
GCCGTCT1850.022.036
CCGTCTT1950.020.87179637
TTACCGG450.003824358620.55555531
TACCGGG450.003824358620.55555532
CTTATAC3750.020.2266651
ACGGGCT851.243905E-619.58823615
TATACAC4350.018.7126433
AGCCGGT500.0070322418.531
CCGCCTT500.0070322418.536
CGCTTGC500.0070322418.51
TTATACA4250.017.847062
TCTTCGC852.7206888E-517.41176613
AGGACCG650.001579111917.0769235
ATGCGTT650.001579111917.07692336
CCGTTAA650.001579111917.07692316
Produced by FastQC (version 0.11.5)