FastQCFastQC Report
Fri 10 Feb 2017
ERR1631171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846607
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG161641.9092684090729228No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT145271.7159083258229617No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT114661.3543474126719954TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC91401.0796036413589778No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA44860.5298798616122947No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC39680.46869444736459775RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC39320.4644421791929431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31560.3727821763817214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30350.35848983058254896No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG21970.25950647703125534No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15680.18520990258762332No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA13340.1575701594718683No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA12980.15331789130021367No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT12740.15048304585244393No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC12340.14575830343949436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10640.12567814818445866No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT10630.12556002962413493No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9800.11575618911726455No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG9240.10914154973913515No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9140.10796036413589775No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9100.10748788989460281No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA8720.10299938460230072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC5700.033.75438737
CTTATAC15700.032.4044571
GTCTATA705.456968E-1231.7142851
TTATACA18150.028.234162
GGTATCA4350.027.6436791
TATACAC19250.027.3896123
TCTCGCG358.867458E-426.4285720
GTATCAA14800.026.01
TAGTCGG509.087395E-625.90000230
CCGTCTT18650.025.49329837
GCCGTCT19150.024.7310736
GCTTTAT7500.023.6799981
AGTCGGT551.9018917E-523.54545431
TCCGAAG400.00193076923.12500221
CCGTCCG400.00193076923.1250029
GTCGGTG502.7012874E-422.232
GATTACG603.7247926E-521.5833341
TGCCGTC22600.020.95575135
ATGCCGT23000.020.59130534
CGGTGTA450.003824988420.55555534
Produced by FastQC (version 0.11.5)