##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631171.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	846607
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91037990472557	31.0	30.0	34.0	28.0	34.0
2	30.89372046297751	31.0	31.0	34.0	27.0	34.0
3	30.28734229695715	31.0	30.0	34.0	26.0	34.0
4	34.533103317123526	35.0	35.0	37.0	30.0	37.0
5	34.66393261572371	35.0	35.0	37.0	32.0	37.0
6	34.90203246606749	35.0	35.0	37.0	32.0	37.0
7	34.930149408166955	35.0	35.0	37.0	32.0	37.0
8	34.96631731133808	36.0	35.0	37.0	32.0	37.0
9	36.65959412100302	38.0	35.0	39.0	32.0	39.0
10	36.427029306396	38.0	35.0	39.0	32.0	39.0
11	36.51048951874955	38.0	35.0	39.0	32.0	39.0
12	36.410046219792655	38.0	35.0	39.0	32.0	39.0
13	36.532276487201266	38.0	35.0	39.0	32.0	39.0
14	37.492416197834416	39.0	36.0	40.0	32.0	41.0
15	37.50406032551113	39.0	36.0	40.0	32.0	41.0
16	37.49348280843414	39.0	36.0	40.0	32.0	41.0
17	37.45017818184825	39.0	36.0	40.0	32.0	41.0
18	37.508684667147804	39.0	36.0	40.0	32.0	41.0
19	37.498023285892984	39.0	36.0	40.0	32.0	41.0
20	37.53581295689736	39.0	37.0	40.0	32.0	41.0
21	37.474282636453516	39.0	36.0	40.0	32.0	41.0
22	37.37031940439897	39.0	36.0	40.0	32.0	41.0
23	37.40964934142997	39.0	36.0	40.0	32.0	41.0
24	37.313895349317924	39.0	36.0	40.0	32.0	41.0
25	37.295757063194614	39.0	36.0	40.0	32.0	41.0
26	37.13158643857185	39.0	36.0	40.0	31.0	41.0
27	37.036552969677786	39.0	36.0	40.0	31.0	41.0
28	37.019601775085725	39.0	36.0	40.0	31.0	41.0
29	36.95180408383111	39.0	36.0	40.0	31.0	41.0
30	36.86969987255007	39.0	36.0	40.0	31.0	41.0
31	36.821175586783475	39.0	36.0	40.0	31.0	41.0
32	36.71826597228702	38.0	35.0	40.0	30.0	41.0
33	36.67537712303347	38.0	35.0	40.0	30.0	41.0
34	36.57902308863498	38.0	35.0	40.0	30.0	41.0
35	36.53365493080024	38.0	35.0	40.0	30.0	41.0
36	36.523157734344274	38.0	35.0	40.0	30.0	41.0
37	36.435079086282066	38.0	35.0	40.0	30.0	41.0
38	36.380471694658794	38.0	35.0	40.0	30.0	41.0
39	36.2985623790023	38.0	35.0	40.0	30.0	41.0
40	36.16164288743183	38.0	35.0	40.0	30.0	41.0
41	36.12975087614442	38.0	35.0	40.0	30.0	41.0
42	36.06072947660485	38.0	35.0	40.0	29.0	41.0
43	35.273270832865784	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	16.0
11	10.0
12	12.0
13	10.0
14	11.0
15	8.0
16	15.0
17	23.0
18	35.0
19	63.0
20	171.0
21	296.0
22	579.0
23	1113.0
24	1899.0
25	3143.0
26	4717.0
27	7213.0
28	10570.0
29	14781.0
30	19962.0
31	25901.0
32	33625.0
33	43223.0
34	56584.0
35	75276.0
36	104589.0
37	147609.0
38	199805.0
39	95337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.37683836774324	22.75447757932547	15.502234212568524	23.366449840362765
2	14.643394160454616	22.34814973181181	39.31576280375664	23.692693303976935
3	16.028688635931427	28.49775633794665	33.660600491136975	21.812954534984947
4	12.112585886958176	19.113472957346207	37.36090063039876	31.41304052529686
5	12.813501423919243	38.128789391063385	35.07814133358217	13.979567851435199
6	27.817157193361265	39.72846905352779	17.256649189057025	15.197724564053924
7	24.54598178375563	33.11040423714899	22.191406402262206	20.15220757683317
8	25.544083618491225	33.306953521527696	22.948428255377053	18.200534604604023
9	24.075279320865526	14.739070194316845	19.00326834056416	42.182382144253474
10	14.946486386245331	28.639026136093843	32.76726981940853	23.647217658252295
11	32.165928228800375	22.883226810078348	24.404475748487787	20.54636921263349
12	21.497577978920564	26.30984624507003	27.868656885662414	24.323918890346995
13	30.203270230461122	20.12031556554576	25.76012246532334	23.916291738669774
14	21.77303046159552	22.398940712751017	26.516908081317542	29.311120744335923
15	24.869036046241057	28.46491937817665	22.172625551170732	24.493419024411565
16	24.564880753407426	26.731529505425776	23.394562057719813	25.309027683446985
17	21.91252848133786	29.9121079792631	23.92893042462441	24.246433114774625
18	24.041497412612937	25.210989278378282	25.84386852459287	24.90364478441591
19	25.017865432248964	28.002957688750506	25.735790041896657	21.243386837103877
20	23.753288125423012	25.166576699696552	26.090972552790138	24.989162622090298
21	25.34304582882022	25.08979963548612	25.660430400410107	23.90672413528355
22	21.236772197725745	30.039676024412742	27.570053165163998	21.153498612697508
23	23.738877661063515	25.031685303806846	25.443092249414427	25.786344785715215
24	23.245968908832552	29.22560290666153	26.04089028321287	21.487537901293045
25	23.660210699887905	27.361455787632277	25.980767936008085	22.99756557647173
26	25.303121755430798	27.85212028721709	24.94345073924501	21.901307218107103
27	22.346141716286304	27.669981467197886	28.763523098675066	21.220353717840744
28	21.91252848133786	25.175553710281157	26.668572312773225	26.243345495607763
29	22.169908824283286	28.625088145975642	27.347990271755375	21.8570127579857
30	23.64072113743449	24.558856706830916	28.39983605143827	23.400586104296327
31	23.316249452225176	26.672942699505203	25.268394898695618	24.742412949574007
32	24.026968829693114	26.103020645943158	28.276402155899966	21.593608368463762
33	22.78223544100155	25.594047769508165	28.066977948445977	23.556738841044307
34	22.641438117095653	29.124139063343442	25.85449919502201	22.379923624538893
35	23.750689517095893	24.76249310482904	29.83214171392393	21.654675664151135
36	24.796393131641956	25.39773472225011	25.65263457542874	24.15323757067919
37	22.962956838296872	25.882020819577445	28.473660151640605	22.68136219048508
38	22.739122166483387	24.260725460573795	28.14458184257867	24.855570530364147
39	25.374229128745686	25.13232231720267	26.204012015019956	23.289436539031687
40	21.81248206074365	24.267812574193222	31.42603356693247	22.493671798130656
41	22.868343871477556	25.643066972042515	25.758705042599463	25.72988411388047
42	22.89562925891234	24.41557889315822	29.52444286428059	23.164348983648846
43	23.847901092242328	24.668706967931993	28.56413896884859	22.919252970977087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	21.0
2	31.0
3	119.0
4	207.0
5	207.0
6	243.5
7	280.0
8	298.0
9	316.0
10	425.5
11	535.0
12	535.0
13	928.5
14	1322.0
15	3084.5
16	4847.0
17	5520.0
18	6193.0
19	6193.0
20	6232.5
21	6272.0
22	7548.0
23	8824.0
24	11255.0
25	13686.0
26	13686.0
27	16650.5
28	19615.0
29	23000.0
30	26385.0
31	30005.0
32	33625.0
33	33625.0
34	36987.5
35	40350.0
36	42826.0
37	45302.0
38	47013.0
39	48724.0
40	48724.0
41	49818.0
42	50912.0
43	51867.5
44	52823.0
45	54468.0
46	56113.0
47	56113.0
48	59866.0
49	63619.0
50	75397.5
51	87176.0
52	70699.0
53	54222.0
54	54222.0
55	62408.5
56	70595.0
57	59550.0
58	48505.0
59	40030.5
60	31556.0
61	31556.0
62	28731.0
63	25906.0
64	22427.5
65	18949.0
66	15830.5
67	12712.0
68	12712.0
69	10504.0
70	8296.0
71	6922.0
72	5548.0
73	3638.5
74	1729.0
75	1729.0
76	1203.5
77	678.0
78	537.5
79	397.0
80	296.0
81	195.0
82	195.0
83	134.0
84	73.0
85	57.0
86	41.0
87	31.5
88	22.0
89	22.0
90	16.5
91	11.0
92	7.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	846607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.586495303150784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.68945479435139	42.14072676065756
2	11.381992271860186	11.743141817456278
3	2.9857572232772585	4.620742529248225
4	1.1777403314610642	2.430219843089897
5	0.6047089690720645	1.5597408196404603
6	0.37293237639014293	1.154296456982578
7	0.2507721586917547	0.9055519750559229
8	0.17149824544027806	0.7077594746322803
9	0.14374820785613404	0.667391962446612
>10	1.006032702084897	10.250762705283488
>50	0.1258765418074721	4.517483125713247
>100	0.08033158152348158	7.843068613914671
>500	0.00526389280573042	1.9070778298065156
>1k	0.0029752437597606717	3.49573871495952
>5k	2.2886490459697476E-4	1.079099323000777
>10k+	6.865947137909244E-4	4.977198048112009
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	16164	1.9092684090729228	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	14527	1.7159083258229617	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	11466	1.3543474126719954	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	9140	1.0796036413589778	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4486	0.5298798616122947	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	3968	0.46869444736459775	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	3932	0.4644421791929431	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3156	0.3727821763817214	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3035	0.35848983058254896	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	2197	0.25950647703125534	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1568	0.18520990258762332	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1334	0.1575701594718683	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1298	0.15331789130021367	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	1274	0.15048304585244393	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1234	0.14575830343949436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1064	0.12567814818445866	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	1063	0.12556002962413493	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	980	0.11575618911726455	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	924	0.10914154973913515	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	914	0.10796036413589775	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	910	0.10748788989460281	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	872	0.10299938460230072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1811856032373935E-4	0.0
4	0.0	0.0	0.0	1.1811856032373935E-4	0.0
5	1.1811856032373935E-4	0.0	0.0	1.1811856032373935E-4	0.0
6	1.1811856032373935E-4	0.0	0.0	1.1811856032373935E-4	0.0
7	1.1811856032373935E-4	0.0	0.0	2.362371206474787E-4	0.0
8	1.1811856032373935E-4	0.0	0.0	2.362371206474787E-4	0.0
9	1.1811856032373935E-4	0.0	0.0	3.5435568097121804E-4	0.0
10	2.362371206474787E-4	0.0	0.0	4.724742412949574E-4	0.0
11	2.362371206474787E-4	0.0	0.0	5.905928016186968E-4	0.0
12	2.362371206474787E-4	0.0	0.0	7.087113619424361E-4	0.0
13	2.362371206474787E-4	0.0	0.0	7.087113619424361E-4	0.0
14	2.362371206474787E-4	0.0	0.0	7.087113619424361E-4	0.0
15	2.362371206474787E-4	0.0	0.0	7.087113619424361E-4	0.0
16	2.362371206474787E-4	0.0	0.0	8.268299222661755E-4	0.0
17	2.362371206474787E-4	0.0	0.0	9.449484825899148E-4	0.0
18	2.362371206474787E-4	0.0	0.0	0.0010630670429136542	0.0
19	2.362371206474787E-4	0.0	0.0	0.0011811856032373936	0.0
20	2.362371206474787E-4	0.0	0.0	0.0014174227238848722	0.0
21	2.362371206474787E-4	0.0	0.0	0.0020080155255035688	0.0
22	2.362371206474787E-4	0.0	0.0	0.0021261340858273084	0.0
23	2.362371206474787E-4	0.0	0.0	0.002362371206474787	0.0
24	2.362371206474787E-4	0.0	0.0	0.0031892011287409624	0.0
25	2.362371206474787E-4	0.0	0.0	0.0035435568097121803	0.0
26	2.362371206474787E-4	0.0	0.0	0.00366167537003592	0.0
27	3.5435568097121804E-4	0.0	0.0	0.004488505292302095	0.0
28	5.905928016186968E-4	0.0	0.0	0.009449484825899149	0.0
29	5.905928016186968E-4	0.0	0.0	0.02173381509956804	0.0
30	5.905928016186968E-4	0.0	0.0	0.04240456315622243	0.0
31	5.905928016186968E-4	0.0	0.0	0.08398229639017868	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	570	0.0	33.754387	37
CTTATAC	1570	0.0	32.404457	1
GTCTATA	70	5.456968E-12	31.714285	1
TTATACA	1815	0.0	28.23416	2
GGTATCA	435	0.0	27.643679	1
TATACAC	1925	0.0	27.389612	3
TCTCGCG	35	8.867458E-4	26.42857	20
GTATCAA	1480	0.0	26.0	1
TAGTCGG	50	9.087395E-6	25.900002	30
CCGTCTT	1865	0.0	25.493298	37
GCCGTCT	1915	0.0	24.73107	36
GCTTTAT	750	0.0	23.679998	1
AGTCGGT	55	1.9018917E-5	23.545454	31
TCCGAAG	40	0.001930769	23.125002	21
CCGTCCG	40	0.001930769	23.125002	9
GTCGGTG	50	2.7012874E-4	22.2	32
GATTACG	60	3.7247926E-5	21.583334	1
TGCCGTC	2260	0.0	20.955751	35
ATGCCGT	2300	0.0	20.591305	34
CGGTGTA	45	0.0038249884	20.555555	34
>>END_MODULE