FastQCFastQC Report
Fri 10 Feb 2017
ERR1631170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432623
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44380.3097814288895264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37310.2604313905333085No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT36930.2577789132242049No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG26950.18811648284300894No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT17860.1246664335278716RNA PCR Primer, Index 29 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17660.12327039283886967No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT16670.11635999142831016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.027.529761
ACCGTCC451.3234344E-424.6666688
CGTCCGG451.3234344E-424.66666833
GTATCAA26250.024.5257151
GATTACG1250.023.6800021
TAGCGGG502.702553E-422.228
TTACGGG1350.021.9259263
TATACAC7950.021.6415083
CTTATAC8000.020.81251
CCTACCG450.00382626120.55555724
CTACCGA500.00703570818.525
ACGTCCG609.238333E-418.532
TTATACA9200.018.4999982
CCGTCCC752.0677145E-417.2666669
GCCGTCT3550.017.19718436
CCGTCTT3600.016.95833437
TAGGACC1606.2937033E-1016.18754
AGAGACG1059.346235E-615.8571428
TTAACGG1557.212293E-915.51612935
TACACAT11650.015.2446355
Produced by FastQC (version 0.11.5)