##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631170.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1432623
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46135654669791	31.0	31.0	34.0	28.0	34.0
2	31.445168058868244	31.0	31.0	34.0	28.0	34.0
3	30.869058363575064	31.0	31.0	34.0	27.0	34.0
4	35.03264850557334	37.0	35.0	37.0	32.0	37.0
5	35.26576286992461	37.0	35.0	37.0	33.0	37.0
6	35.469156226027366	37.0	35.0	37.0	33.0	37.0
7	35.49493481537013	37.0	35.0	37.0	33.0	37.0
8	35.51400193910052	37.0	35.0	37.0	33.0	37.0
9	37.26984628893994	39.0	37.0	39.0	34.0	39.0
10	37.1034710457671	39.0	37.0	39.0	33.0	39.0
11	37.17642534009296	39.0	37.0	39.0	33.0	39.0
12	37.11471894559839	39.0	37.0	39.0	33.0	39.0
13	37.1697627359047	39.0	37.0	39.0	33.0	39.0
14	38.33548812213681	40.0	38.0	41.0	34.0	41.0
15	38.347901715943415	40.0	38.0	41.0	34.0	41.0
16	38.34622507107592	40.0	38.0	41.0	34.0	41.0
17	38.33916529331164	40.0	38.0	41.0	34.0	41.0
18	38.35338187366809	40.0	38.0	41.0	34.0	41.0
19	38.39450225216264	40.0	38.0	41.0	34.0	41.0
20	38.37509170242276	40.0	38.0	41.0	34.0	41.0
21	38.33796818842082	40.0	38.0	41.0	34.0	41.0
22	38.29769520662449	40.0	38.0	41.0	34.0	41.0
23	38.2654082755896	40.0	38.0	41.0	34.0	41.0
24	38.251406685499255	40.0	38.0	41.0	34.0	41.0
25	38.2192740169605	40.0	38.0	41.0	34.0	41.0
26	38.11558309478488	40.0	37.0	41.0	33.0	41.0
27	38.073827517776834	40.0	37.0	41.0	33.0	41.0
28	38.02135802650104	40.0	37.0	41.0	33.0	41.0
29	37.96435559110806	40.0	37.0	41.0	33.0	41.0
30	37.87958590641083	40.0	37.0	41.0	33.0	41.0
31	37.85087074547875	40.0	37.0	41.0	33.0	41.0
32	37.78955454435675	40.0	37.0	41.0	33.0	41.0
33	37.777182831770816	40.0	37.0	41.0	33.0	41.0
34	37.718724325939206	40.0	37.0	41.0	33.0	41.0
35	37.66144128636773	40.0	37.0	41.0	33.0	41.0
36	37.63092593096719	40.0	37.0	41.0	33.0	41.0
37	37.581635224340246	40.0	37.0	41.0	32.0	41.0
38	37.53075163528716	40.0	37.0	41.0	32.0	41.0
39	37.47199228268707	40.0	37.0	41.0	32.0	41.0
40	37.42882670458313	39.0	37.0	41.0	32.0	41.0
41	37.35381883440375	39.0	36.0	41.0	32.0	41.0
42	37.29352942120851	39.0	36.0	41.0	31.0	41.0
43	36.70052553951737	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	5.0
11	8.0
12	6.0
13	1.0
14	3.0
15	3.0
16	8.0
17	10.0
18	23.0
19	70.0
20	114.0
21	245.0
22	474.0
23	864.0
24	1672.0
25	2932.0
26	4870.0
27	7398.0
28	11142.0
29	16144.0
30	22147.0
31	30135.0
32	39890.0
33	52538.0
34	69555.0
35	94276.0
36	133050.0
37	199981.0
38	353041.0
39	392014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.51435723145587	21.205578857801388	14.890588801101195	23.389475109641545
2	14.656891589762274	22.88354996394725	39.97639295194898	22.4831654943415
3	15.408170886548659	27.876838498334873	33.17446390292492	23.540526712191554
4	12.540703311338714	18.54032777639337	40.14573268752491	28.773236224743005
5	12.789198553981054	38.30903175503953	35.914263557125636	12.987506133853778
6	27.934145968618402	40.49013592550168	18.296020655818033	13.279697450061882
7	25.626630313767123	32.7585135796368	24.027465704515425	17.58739040208066
8	24.47685120230514	35.75448670026937	21.70138270850042	18.06727938892507
9	25.545380745667213	15.881079669948061	20.755774547804968	37.81776503657975
10	14.767527814365678	28.850576878913714	34.22079639933185	22.161098907388755
11	32.17706263266749	24.355674870499776	25.096414060084193	18.37084843674854
12	21.84524470150207	26.160197065103656	30.972768132299983	21.021790101094286
13	27.74302799829404	22.279134147643866	27.265791488758733	22.71204636530336
14	21.959231423759075	21.658035645106914	28.278828414732978	28.103904516401034
15	23.21224774417275	29.179344461173667	24.636907267299215	22.971500527354372
16	22.594220531151603	28.869981844490837	25.28683401006406	23.2489636142935
17	22.052347337715506	28.41242950867046	26.755748023031877	22.779475130582156
18	22.851371226065755	27.72187798185566	27.7152467885829	21.711504003495683
19	24.37221795266445	26.706467786710114	27.915508825420225	21.005805435205215
20	24.18521830237264	26.550739447851946	27.50325800995796	21.760784239817454
21	22.952165363811694	27.122767120170483	27.23766126887534	22.68740624714248
22	22.322551013071827	27.819880038223594	27.470102043594164	22.38746690511042
23	22.479186778377844	27.052965085720388	27.945663304302666	22.522184831599102
24	22.43423426819198	27.408397045140276	27.494881765823948	22.662486920843794
25	22.81828506173641	27.30153013039718	27.62841305772698	22.25177175013943
26	22.844111814482947	27.446718362053378	27.405465359693377	22.303704463770302
27	22.786874146233867	27.65109871892326	26.966619969105622	22.595407165737253
28	22.200118244646358	27.392831191457905	27.783582980309546	22.62346758358619
29	22.89087917756451	27.444345092882077	27.363095524782167	22.301680204771248
30	22.804115248743038	27.54981596693617	27.54841992624717	22.09764885807362
31	23.206803185485644	26.94218925704809	27.17169834632	22.67930921114627
32	22.036292869791982	27.43101290430211	27.841169658730873	22.691524567175033
33	22.50731699826123	27.127234450375294	28.06439656490228	22.3010519864612
34	22.600153704079858	26.700953425988555	27.59127837539953	23.107614494532058
35	22.263358887858146	27.557005576484535	28.221730350552797	21.957905185104526
36	22.97778271045488	27.330148964521722	27.121440881515934	22.57062744350747
37	23.06510505555195	26.856053546536668	27.489157998999037	22.589683398912346
38	23.139793232413552	26.722522254633635	27.47442976973007	22.663254743222748
39	22.80467366501864	26.783738638846366	27.669107643811387	22.742480052323604
40	22.225944997392894	27.14287010609211	28.123728294184865	22.50745660233013
41	22.611950247901923	26.309433814757966	27.77464831989993	23.303967617440176
42	22.632541848064704	26.805167863422547	27.555120921554376	23.00716936695837
43	22.785966719786018	26.117757428158	28.190528841153608	22.90574701090238
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	20.0
2	26.0
3	133.0
4	240.0
5	240.0
6	297.5
7	355.0
8	382.0
9	409.0
10	605.5
11	802.0
12	802.0
13	1303.0
14	1804.0
15	4215.5
16	6627.0
17	7881.5
18	9136.0
19	9136.0
20	9585.0
21	10034.0
22	12816.5
23	15599.0
24	20397.5
25	25196.0
26	25196.0
27	31227.0
28	37258.0
29	45322.0
30	53386.0
31	60643.5
32	67901.0
33	67901.0
34	74679.0
35	81457.0
36	86676.0
37	91895.0
38	97163.0
39	102431.0
40	102431.0
41	104831.0
42	107231.0
43	106416.5
44	105602.0
45	106451.0
46	107300.0
47	107300.0
48	105839.0
49	104378.0
50	106723.0
51	109068.0
52	104563.5
53	100059.0
54	100059.0
55	93384.5
56	86710.0
57	76313.0
58	65916.0
59	55808.0
60	45700.0
61	45700.0
62	40536.0
63	35372.0
64	29210.0
65	23048.0
66	19253.0
67	15458.0
68	15458.0
69	12241.0
70	9024.0
71	7751.0
72	6478.0
73	4968.0
74	3458.0
75	3458.0
76	2544.5
77	1631.0
78	1274.0
79	917.0
80	641.0
81	365.0
82	365.0
83	272.5
84	180.0
85	135.5
86	91.0
87	70.5
88	50.0
89	50.0
90	33.5
91	17.0
92	8.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1432623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.66924920620918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24540421884045	38.367212321799485
2	13.286046027960738	13.19815862255895
3	4.016867384070673	5.985443613830995
4	1.649080212044999	3.2763430405236535
5	0.8666307544886582	2.1522449457231128
6	0.5299307598906294	1.5792757785026086
7	0.34994257272425244	1.2166969396752012
8	0.263955450490994	1.0488375239819512
9	0.20193051703039835	0.9026763455449371
>10	1.2961816290235755	12.86583895053919
>50	0.17487722286045787	6.063187664193954
>100	0.11291191236284617	10.118789197514905
>500	0.004681003658553834	1.4812487056920238
>1k	0.001560334552851278	1.744046349919157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4438	0.3097814288895264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3731	0.2604313905333085	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	3693	0.2577789132242049	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	2695	0.18811648284300894	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	1786	0.1246664335278716	RNA PCR Primer, Index 29 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1766	0.12327039283886967	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	1667	0.11635999142831016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.980203445009608E-5	0.0
5	0.0	0.0	0.0	6.980203445009608E-5	0.0
6	0.0	0.0	0.0	6.980203445009608E-5	0.0
7	0.0	0.0	0.0	6.980203445009608E-5	0.0
8	0.0	0.0	0.0	6.980203445009608E-5	0.0
9	0.0	0.0	0.0	6.980203445009608E-5	0.0
10	0.0	0.0	0.0	1.3960406890019215E-4	0.0
11	0.0	0.0	0.0	1.3960406890019215E-4	0.0
12	0.0	0.0	0.0	1.3960406890019215E-4	0.0
13	0.0	0.0	0.0	2.0940610335028824E-4	0.0
14	0.0	0.0	0.0	2.0940610335028824E-4	0.0
15	0.0	0.0	0.0	2.792081378003843E-4	0.0
16	0.0	0.0	0.0	4.188122067005765E-4	0.0
17	0.0	0.0	0.0	4.886142411506726E-4	0.0
18	0.0	0.0	0.0	4.886142411506726E-4	0.0
19	0.0	0.0	0.0	4.886142411506726E-4	0.0
20	0.0	0.0	0.0	6.282183100508647E-4	0.0
21	0.0	0.0	0.0	7.678223789510569E-4	0.0
22	0.0	0.0	0.0	8.37624413401153E-4	0.0
23	0.0	0.0	0.0	0.0012564366201017294	0.0
24	0.0	0.0	0.0	0.0016054467923522098	0.0
25	0.0	0.0	0.0	0.0018148528957024981	0.0
26	0.0	0.0	0.0	0.0020242589990527864	0.0
27	0.0	0.0	0.0	0.002792081378003843	0.0
28	0.0	0.0	0.0	0.009911888891913644	0.0
29	0.0	0.0	0.0	0.022197046955130553	0.0
30	0.0	0.0	0.0	0.04118320032555669	0.0
31	0.0	0.0	0.0	0.08962581223392337	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	840	0.0	27.52976	1
ACCGTCC	45	1.3234344E-4	24.666668	8
CGTCCGG	45	1.3234344E-4	24.666668	33
GTATCAA	2625	0.0	24.525715	1
GATTACG	125	0.0	23.680002	1
TAGCGGG	50	2.702553E-4	22.2	28
TTACGGG	135	0.0	21.925926	3
TATACAC	795	0.0	21.641508	3
CTTATAC	800	0.0	20.8125	1
CCTACCG	45	0.003826261	20.555557	24
CTACCGA	50	0.007035708	18.5	25
ACGTCCG	60	9.238333E-4	18.5	32
TTATACA	920	0.0	18.499998	2
CCGTCCC	75	2.0677145E-4	17.266666	9
GCCGTCT	355	0.0	17.197184	36
CCGTCTT	360	0.0	16.958334	37
TAGGACC	160	6.2937033E-10	16.1875	4
AGAGACG	105	9.346235E-6	15.857142	8
TTAACGG	155	7.212293E-9	15.516129	35
TACACAT	1165	0.0	15.244635	5
>>END_MODULE