FastQCFastQC Report
Fri 10 Feb 2017
ERR1631169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358275
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG113443.166282883260066No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT97192.7127206754587956TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC63611.7754518177377714No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT35600.9936501290907822No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC33580.9372688577210244RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC30140.8412532272695555No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG16350.4563533598492778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12420.34666108436257065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.31930779429209405No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA8540.23836438489986742No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT8180.22831623752703928No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT7970.22245481822622284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4630.12923033982276186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC5200.033.086541
GCAACGC451.07644155E-732.888893
CGTCTTC4750.031.93684237
GGTATCA2400.026.2083341
TTATACA6750.026.0370392
CCGTCTT15000.025.15999837
GCCGTCT15350.024.58631936
TATACAC7850.023.8025473
GTATCAA6050.022.9338861
GTATAGA502.6970747E-422.1999991
ATACACA19850.021.6221661
TACACAT19950.021.5137842
ATCTCGC555.1336014E-420.18181837
AGCAACG759.236608E-619.7333342
TGCCGTC19200.019.6562535
TACGGGT851.240418E-619.5882364
TAATACT951.668086E-719.4736844
ATGCCGT20200.018.68316734
ATAATAC1156.368464E-817.6956523
TATGCCG21750.017.4367833
Produced by FastQC (version 0.11.5)