##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631169.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358275
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.680781522573444	31.0	30.0	31.0	25.0	34.0
2	29.633253785499964	31.0	30.0	31.0	25.0	34.0
3	29.12453841323006	31.0	28.0	31.0	23.0	34.0
4	33.26972856046333	35.0	33.0	37.0	28.0	37.0
5	32.48267113250994	35.0	32.0	37.0	25.0	37.0
6	32.844984997557745	35.0	32.0	37.0	26.0	37.0
7	33.015616495708606	35.0	32.0	36.0	26.0	37.0
8	33.12394948014793	35.0	32.0	37.0	27.0	37.0
9	34.59268718163422	37.0	33.0	39.0	28.0	39.0
10	34.12559067755216	37.0	32.0	39.0	27.0	39.0
11	34.44061126229851	37.0	33.0	39.0	27.0	39.0
12	34.266778312748585	37.0	32.0	39.0	27.0	39.0
13	34.50183239131952	37.0	33.0	39.0	27.0	39.0
14	35.167965947945014	37.0	33.0	39.0	27.0	40.0
15	35.17860581955202	37.0	33.0	39.0	27.0	40.0
16	35.182052892331306	37.0	33.0	39.0	27.0	40.0
17	35.01139627381202	37.0	33.0	39.0	27.0	40.0
18	35.148517200474494	37.0	33.0	39.0	27.0	40.0
19	35.153552438769104	37.0	33.0	39.0	27.0	40.0
20	35.17942920940618	37.0	33.0	39.0	27.0	40.0
21	35.10053450561719	37.0	33.0	39.0	27.0	40.0
22	34.862354336752496	37.0	33.0	39.0	27.0	40.0
23	34.89181773777126	37.0	33.0	39.0	27.0	40.0
24	34.774832181983115	37.0	33.0	39.0	27.0	40.0
25	34.69504989184286	37.0	32.0	39.0	26.0	40.0
26	34.436829251273466	37.0	32.0	39.0	25.0	40.0
27	34.31437303747121	37.0	32.0	39.0	25.0	40.0
28	34.194163701067616	37.0	32.0	39.0	25.0	40.0
29	34.16470588235294	37.0	32.0	39.0	25.0	40.0
30	34.02308840974112	37.0	32.0	39.0	24.0	40.0
31	33.99273184006699	37.0	32.0	39.0	24.0	40.0
32	33.84927778940758	36.0	31.0	39.0	24.0	40.0
33	33.66112902100342	36.0	31.0	39.0	24.0	40.0
34	33.501999860442396	36.0	31.0	39.0	24.0	40.0
35	33.48103551740981	36.0	31.0	39.0	24.0	40.0
36	33.54939641336962	36.0	31.0	39.0	24.0	40.0
37	33.44085967483078	36.0	31.0	39.0	24.0	40.0
38	33.338075500662896	36.0	31.0	39.0	23.0	40.0
39	33.21011792617403	36.0	31.0	39.0	23.0	40.0
40	32.92600097690322	36.0	30.0	38.0	22.0	40.0
41	32.966955550903634	36.0	30.0	38.0	22.0	40.0
42	32.873491033424045	35.0	30.0	38.0	22.0	40.0
43	31.984006698764915	35.0	29.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	21.0
11	13.0
12	14.0
13	8.0
14	22.0
15	21.0
16	53.0
17	76.0
18	138.0
19	287.0
20	455.0
21	840.0
22	1403.0
23	2167.0
24	3455.0
25	4851.0
26	7001.0
27	9618.0
28	12775.0
29	16570.0
30	20372.0
31	24749.0
32	29093.0
33	33737.0
34	37097.0
35	39182.0
36	38524.0
37	34082.0
38	28818.0
39	12824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.033144930570096	26.24631916823669	16.95010815714186	21.770427744051357
2	15.801269974181844	23.83895052682995	37.531226013537086	22.82855348545112
3	17.508338566743422	30.091131114367453	32.83260065592073	19.56792966296839
4	13.382736724583072	19.286302421324404	34.277859186379175	33.05310166771335
5	12.506035866303817	39.79233828762822	32.93810620333543	14.763519642732536
6	27.284348614890796	38.87404926383365	17.369339194752634	16.472262926522923
7	23.00746633172842	35.89896029586212	21.134045077105576	19.959528295303887
8	25.656269625287837	33.334170678947736	23.21819831135301	17.791361384411417
9	22.759053799455724	14.972856046333124	18.458446723885285	43.80964343032587
10	14.277859186379176	27.480008373456144	33.230618937966646	25.011513502198028
11	31.12134533528714	22.58935175493685	23.137813132370386	23.151489777405622
12	19.559277091619563	28.32489009838811	27.636033772939783	24.479799037052544
13	31.18247156513851	20.05638127136976	24.363687111855416	24.397460051636312
14	22.186867629614124	24.226083315888634	25.13683622915358	28.450212825343662
15	24.994208359500384	29.528713976693883	21.26802037540995	24.209057288395787
16	25.005652082897218	27.430604982206408	22.085827925476238	25.477915009420137
17	20.0002791152048	32.02205010117926	23.654734491661433	24.322936291954505
18	23.891982415742095	24.630521247644964	24.162724164398856	27.31477217221408
19	26.234596329635057	28.663735957016257	24.076477566115415	21.025190147233268
20	21.40729886260554	26.10313306817389	25.124555160142346	27.365012909078224
21	26.459842299909287	24.95708603726188	26.92596469192659	21.65710697090224
22	20.226641546298236	31.572395506245204	24.511897285604633	23.68906566185193
23	24.058893308212966	27.188332984439327	26.208359500383782	22.544414206963925
24	23.97822901402554	27.862117088828413	27.35105714883818	20.808596748307863
25	23.52215476938106	27.501500244225802	24.652013118414626	24.324331867978508
26	24.586979275696045	28.737422371083664	26.870141650966435	19.805456702253853
27	21.19963715023376	28.821156932523902	25.283650826878795	24.69555509036355
28	20.451608401367665	26.994627032307584	31.06328937268858	21.490475193636176
29	21.83601981717954	25.03830856185891	27.355522992114995	25.770148628846556
30	21.89993719907892	28.234177656827857	28.366757379108225	21.499127764984998
31	21.373246807619843	26.348196217988974	29.01877049752285	23.259786476868328
32	25.98562556695276	26.37024631916824	26.90475193636173	20.739376177517272
33	20.130346800641966	29.773777126508964	29.84969646221478	20.24617961063429
34	21.204940339124974	30.20054427464936	27.072221059242203	21.522294326983463
35	22.424115553694786	24.683832251761913	33.259646919265926	19.63240527527737
36	23.578256925546018	26.41825413439397	24.391598632335494	25.61189030772451
37	22.13216104947317	25.277231177168375	29.11729816481753	23.473309608540927
38	22.066010745935387	24.323494522364104	27.555648593957155	26.054846137743354
39	26.505338078291814	25.568906566185195	26.13551043193078	21.790244923592212
40	20.773986462912568	23.945293419859045	33.451399064964065	21.829321052264323
41	23.322587397948503	26.730025818156445	25.53178424394669	24.415602539948363
42	21.29230339822762	24.170260274928477	32.44211848440444	22.095317842439467
43	24.208219942781383	23.581048077594026	28.80999232433187	23.40073965529272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	6.0
2	8.0
3	23.5
4	39.0
5	39.0
6	67.0
7	95.0
8	102.5
9	110.0
10	159.0
11	208.0
12	208.0
13	378.5
14	549.0
15	1257.5
16	1966.0
17	2330.0
18	2694.0
19	2694.0
20	2697.0
21	2700.0
22	3313.0
23	3926.0
24	5045.0
25	6164.0
26	6164.0
27	7430.0
28	8696.0
29	10071.0
30	11446.0
31	13165.0
32	14884.0
33	14884.0
34	16431.5
35	17979.0
36	19223.0
37	20467.0
38	21207.5
39	21948.0
40	21948.0
41	22337.5
42	22727.0
43	23032.0
44	23337.0
45	23531.5
46	23726.0
47	23726.0
48	24484.0
49	25242.0
50	31179.5
51	37117.0
52	28892.0
53	20667.0
54	20667.0
55	27748.5
56	34830.0
57	28914.5
58	22999.0
59	17101.0
60	11203.0
61	11203.0
62	9616.0
63	8029.0
64	6720.5
65	5412.0
66	4522.0
67	3632.0
68	3632.0
69	2940.0
70	2248.0
71	1816.0
72	1384.0
73	1111.0
74	838.0
75	838.0
76	662.5
77	487.0
78	382.0
79	277.0
80	203.5
81	130.0
82	130.0
83	96.5
84	63.0
85	47.0
86	31.0
87	20.5
88	10.0
89	10.0
90	6.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	358275.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.99465953122137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.01318849109971	62.10429293263078
2	6.404542483136474	8.837584561544883
3	1.5546855024673092	3.217949907625735
4	0.6288314969406951	1.7354406013572607
5	0.3474832636268499	1.1987244733366074
6	0.2227477711014744	0.9221043977090794
7	0.1415824898242815	0.683790497670624
8	0.10398690756669075	0.5739633026614732
9	0.08339815139541411	0.5178624354953866
>10	0.4576973203388528	5.649363159773841
>50	0.026965001996697296	1.2201161692667633
>100	0.010061567909140012	1.1637475797374461
>500	0.0012073881490968015	0.6855864410335849
>1k	0.002414776298193603	3.8744380768495787
>5k	8.04925432731201E-4	4.465058716816543
>10k+	4.024627163656005E-4	3.1499767464904767
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	11344	3.166282883260066	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	9719	2.7127206754587956	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	6361	1.7754518177377714	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	3560	0.9936501290907822	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	3358	0.9372688577210244	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	3014	0.8412532272695555	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1635	0.4563533598492778	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1242	0.34666108436257065	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1144	0.31930779429209405	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	854	0.23836438489986742	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	818	0.22831623752703928	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	797	0.22245481822622284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	463	0.12923033982276186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.791152048007815E-4	0.0
13	0.0	0.0	0.0	2.791152048007815E-4	0.0
14	0.0	0.0	0.0	2.791152048007815E-4	0.0
15	0.0	0.0	0.0	5.58230409601563E-4	0.0
16	0.0	0.0	0.0	5.58230409601563E-4	0.0
17	0.0	0.0	0.0	5.58230409601563E-4	0.0
18	0.0	0.0	0.0	5.58230409601563E-4	0.0
19	0.0	0.0	0.0	5.58230409601563E-4	0.0
20	0.0	0.0	0.0	8.373456144023446E-4	0.0
21	0.0	0.0	0.0	8.373456144023446E-4	0.0
22	0.0	0.0	0.0	8.373456144023446E-4	0.0
23	0.0	0.0	0.0	0.001116460819203126	0.0
24	0.0	0.0	0.0	0.001116460819203126	0.0
25	0.0	0.0	0.0	0.0013955760240039075	0.0
26	0.0	0.0	0.0	0.0013955760240039075	0.0
27	0.0	0.0	0.0	0.0019538064336054707	0.0
28	0.0	0.0	0.0	0.004186728072011723	0.0
29	0.0	0.0	0.0	0.009489916963226572	0.0
30	0.0	0.0	0.0	0.016188681878445327	0.0
31	0.0	0.0	0.0	0.03516851580489847	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	520	0.0	33.08654	1
GCAACGC	45	1.07644155E-7	32.88889	3
CGTCTTC	475	0.0	31.936842	37
GGTATCA	240	0.0	26.208334	1
TTATACA	675	0.0	26.037039	2
CCGTCTT	1500	0.0	25.159998	37
GCCGTCT	1535	0.0	24.586319	36
TATACAC	785	0.0	23.802547	3
GTATCAA	605	0.0	22.933886	1
GTATAGA	50	2.6970747E-4	22.199999	1
ATACACA	1985	0.0	21.622166	1
TACACAT	1995	0.0	21.513784	2
ATCTCGC	55	5.1336014E-4	20.181818	37
AGCAACG	75	9.236608E-6	19.733334	2
TGCCGTC	1920	0.0	19.65625	35
TACGGGT	85	1.240418E-6	19.588236	4
TAATACT	95	1.668086E-7	19.473684	4
ATGCCGT	2020	0.0	18.683167	34
ATAATAC	115	6.368464E-8	17.695652	3
TATGCCG	2175	0.0	17.43678	33
>>END_MODULE