FastQCFastQC Report
Fri 10 Feb 2017
ERR1631168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607940
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT129712.1335987103990526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128362.1113925716353585No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA63401.0428660723097676No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT59010.9706549988485706No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG43710.7189854261933744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43610.7173405270256933No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA42900.705661742935158No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT33910.5577853077606343No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT32920.5415008060005921TruSeq Adapter, Index 6 (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA30620.5036681251439287No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT28720.47241504095798925No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT27880.4585978879494687No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC26660.4385301181037603No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG26340.43326644076718096No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC25510.4196137776754285No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC24090.396256209494358No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG22470.36960884297792546No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG21020.3457578050465507No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG18570.30545777543836566No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG17680.29081817284600453No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT17360.28555449550942524No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG16970.2791393887554693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16460.27075040300029607No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG16310.2682830542487745No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG16300.2681185643320064No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC15920.26186794749481856No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG15890.2613744777445142No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14580.2398262986478929No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14360.23620752047899463No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG14180.23324670197716882No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA14130.23242425239332828No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG14010.23045037339211108No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13570.2232128170543146No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG13140.21613975063328617No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC13020.21416587163206896No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG12840.21120505313024313No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT12330.2028160673750699No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT12300.20232259762476562No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC12210.20084218837385265No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC12090.19886830937263544RNA PCR Primer, Index 6 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.19738790012172255No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11800.1940981017863605No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA11790.1939336118695924No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10610.17452380169095635No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA10440.17172747310589861No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG10040.1651478764351745No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA9990.164325426851334No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG9820.16152909826627626No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9820.16152909826627626No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9800.16120011843274007No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG9560.15725236043030563No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9550.1570878705135375No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG9350.15379807217817548No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC9090.14952133434220483No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9010.14820541500806No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8970.1475474553409876No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG8850.14557357633977036No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG8780.14442214692239363No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8780.14442214692239363No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC8770.14425765700562557No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG8670.14261275783794453No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA8660.14244826792117643No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8610.14162581833733592No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT8330.13702010066782908No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8100.1332368325821627No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG8090.1330723426653946No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8090.1330723426653946No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA8050.1324143829983222No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7970.13109846366417738No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC7880.12961805441326446No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG7870.12945356449649636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC7820.12863111491265586No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA7630.12550580649406193No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC7600.1250123367437576No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG7560.1243543770766852No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA7540.124025397243149No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA7260.11941967957364215No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7200.11843274007303352No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA7160.11777478040596112No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG6840.11251110306938183No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6790.11168865348554133No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6690.11004375431786032No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA6650.10938579465078789No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT6630.10905681481725171No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC6580.1082343652334112No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6510.10708293581603448No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG6430.10576701648188966No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA6370.10478007698128106No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT6340.10428660723097674No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6320.10395762739744054No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG6280.10329966773036812No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA6240.10264170806329573No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA6230.10247721814652762No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6140.10099680889561469No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA6110.10050333914531039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAT251.2329416E-437.03
ACGTTTA403.828245E-837.026
CGTGCGT200.001841254437.012
CATGAGT200.001841254437.01
GGCAATT251.2329416E-437.013
AGAAGTC251.2329416E-437.032
GCTGCGT200.001841254437.08
ATTCGTC251.2329416E-437.017
CGTCTCC251.2329416E-437.020
TGAGTAT200.001841254437.03
GCACCCC200.001841254437.029
AATTCGT251.2329416E-437.016
GTTTTAG251.2329416E-437.01
ACACTAG251.2329416E-437.027
CACGTTT403.828245E-837.025
AATAATT251.2329416E-437.04
TCACGTT403.828245E-837.024
TTACTTT750.036.9999963
TCAGAGT1050.035.2380943
TGATCAC451.0785516E-732.8888921
Produced by FastQC (version 0.11.5)