FastQCFastQC Report
Fri 10 Feb 2017
ERR1631167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495216
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG114832.31878614584343No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT87481.766501890084327Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC64801.3085199185809828No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT58851.1883703272915254No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC31350.6330570902394107Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC28750.5805547478272107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17310.34954444121353107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16120.32551452295563954No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG15800.31905269619721494No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA8570.173055797874059No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT8280.1671997673742367No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT7810.15770895932280057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6550.13226551646150367No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT5300.10702400568640753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC8650.031.6531771
CGGTCGT303.5965454E-430.83333410
CTCGCCA250.0054933629.613
CCGGTCG250.0054933629.69
CGTCTTC4450.029.51685337
TTATACA10050.027.7960192
GTATCAA8050.026.4285721
TATACAC11350.026.2422923
GGTATCA2500.025.9000021
CCGTCTT13400.025.12686337
GGTCGTG451.3217264E-424.66666811
GCCGTCT13600.024.62132536
TTCGTCC400.001929643923.12523
TCGTCCA400.001929643923.12524
CGCTTAA450.003822778820.5555571
CCGTACA1351.8189894E-1220.55555517
TGCCGTC16500.020.29393835
GTCCAAA555.1374146E-420.18181823
CGTTCGC951.6713057E-719.47368434
GCCCCGT1551.8189894E-1219.09677314
Produced by FastQC (version 0.11.5)