##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631167.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495216
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.144316823365966	31.0	30.0	31.0	26.0	34.0
2	30.115789877548384	31.0	30.0	33.0	26.0	34.0
3	29.548903912636103	31.0	30.0	33.0	25.0	34.0
4	33.79733288100546	35.0	33.0	37.0	28.0	37.0
5	33.415846822396695	35.0	33.0	37.0	28.0	37.0
6	33.73223603437692	35.0	33.0	37.0	29.0	37.0
7	33.83289716002714	35.0	33.0	37.0	29.0	37.0
8	33.900403056444055	35.0	33.0	37.0	29.0	37.0
9	35.46916093179542	37.0	34.0	39.0	30.0	39.0
10	35.071605117766794	37.0	34.0	39.0	29.0	39.0
11	35.30696504151724	37.0	34.0	39.0	30.0	39.0
12	35.15994434751704	37.0	34.0	39.0	30.0	39.0
13	35.345130205809184	37.0	34.0	39.0	30.0	39.0
14	36.11428144486446	38.0	34.0	40.0	30.0	41.0
15	36.119549852993444	38.0	34.0	40.0	30.0	41.0
16	36.13327719621337	38.0	34.0	40.0	30.0	41.0
17	36.03676375561371	38.0	34.0	40.0	30.0	41.0
18	36.12601773771445	38.0	34.0	40.0	30.0	41.0
19	36.12754232496527	38.0	34.0	40.0	30.0	41.0
20	36.16647281186391	38.0	34.0	40.0	30.0	41.0
21	36.094379018448514	38.0	34.0	40.0	29.0	41.0
22	35.914619075312594	38.0	34.0	40.0	29.0	41.0
23	35.9370375755226	38.0	34.0	40.0	29.0	41.0
24	35.823549723756905	38.0	34.0	40.0	29.0	41.0
25	35.79326394946852	38.0	34.0	40.0	29.0	41.0
26	35.545576071855514	38.0	34.0	40.0	27.0	41.0
27	35.44740476882815	38.0	34.0	40.0	27.0	41.0
28	35.420293366934835	38.0	34.0	40.0	27.0	41.0
29	35.3309061096572	38.0	34.0	40.0	27.0	41.0
30	35.2270282058738	38.0	34.0	40.0	27.0	40.0
31	35.209985137798455	38.0	34.0	40.0	27.0	40.0
32	35.06357024005686	37.0	33.0	40.0	27.0	40.0
33	34.95895528415883	37.0	33.0	39.0	27.0	40.0
34	34.840722028367416	37.0	33.0	39.0	26.0	40.0
35	34.80209443959807	37.0	33.0	39.0	26.0	40.0
36	34.80654300345708	37.0	33.0	39.0	26.0	40.0
37	34.732516719976736	37.0	33.0	39.0	26.0	40.0
38	34.65440131175083	37.0	33.0	39.0	25.0	40.0
39	34.553915867015604	37.0	33.0	39.0	25.0	40.0
40	34.3833922975025	37.0	33.0	39.0	25.0	40.0
41	34.352001954702594	37.0	33.0	39.0	25.0	40.0
42	34.28609939905011	37.0	33.0	39.0	24.0	40.0
43	33.40852072630933	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	13.0
11	9.0
12	10.0
13	9.0
14	11.0
15	20.0
16	24.0
17	29.0
18	67.0
19	138.0
20	339.0
21	505.0
22	912.0
23	1500.0
24	2390.0
25	3719.0
26	5631.0
27	8158.0
28	11228.0
29	15142.0
30	19748.0
31	25430.0
32	32295.0
33	40252.0
34	50397.0
35	61756.0
36	69548.0
37	68843.0
38	55138.0
39	21942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.55919841039062	24.678524118768376	16.23675325514523	22.52552421569578
2	15.09220219055927	23.709653969177086	39.09647507350328	22.101668766760362
3	16.78883557881813	29.755298697941907	33.42662595715809	20.029239766081872
4	12.557954508739622	19.75360246841782	36.23085683822816	31.45758618461439
5	11.91984911634519	39.79495815967174	34.596620464605344	13.688572259377727
6	26.65766049562211	40.071403185680595	17.792236115149755	15.478700203547543
7	23.294077735775904	34.762002843203774	22.347218183580498	19.596701237439824
8	24.79483700042002	34.63498756098349	23.11960841329844	17.450567025298053
9	23.513375981389938	15.186706406901232	19.067639171593807	42.232278440115024
10	14.24368356434364	28.545321637426902	33.65804012794417	23.55295467028529
11	30.778892442893607	23.423516203030598	24.173693903266454	21.62389745080934
12	19.799642984071596	27.748497625278667	28.750282704920682	23.701576685729055
13	29.926335174953962	20.8244483215405	25.981389938935738	23.267826564569805
14	21.719209395496105	23.86776679267229	26.354358502148557	28.058665309683047
15	24.286170075280282	29.390003554004718	22.663443830570902	23.6603825401441
16	24.003061290426803	28.004951374753645	23.08063552066169	24.91135181415786
17	20.366668282123356	31.327541921101094	24.518997770669767	23.78679202610578
18	22.745630189654616	26.378590352492647	25.567833026396563	25.307946431456173
19	23.574157539336372	29.93360473005719	25.52764854124261	20.96458918936383
20	22.168104423120415	26.121328874672873	26.172619624567865	25.53794707763885
21	24.495371716584277	26.354156570062358	26.87453555620174	22.275936157151627
22	20.78688895350716	30.752439339601306	25.469492100416787	22.991179606474752
23	24.58765467997803	25.764312946269914	26.31518367742561	23.33284869632645
24	21.72769054311654	29.389397757746117	26.04580627443378	22.837105424703562
25	21.55705793027689	27.209338955122615	27.603712319472717	23.629890795127785
26	21.964758812316244	30.36230654906142	26.574868340279796	21.09806629834254
27	20.855747794901617	28.44637491518852	26.367686019837812	24.33019127007205
28	21.163896158443993	26.406658912474555	30.216511582824463	22.212933346256985
29	20.469249781913344	27.434493231236473	26.160099835223416	25.936157151626766
30	22.50250395786889	26.04196956479597	29.50126813350134	21.954258343833803
31	21.81654066104488	26.380407741268456	27.05062033536881	24.752431262317856
32	21.840368647216568	26.68815224063843	29.50793189234597	21.963547219799036
33	21.6650915963943	28.02978902135634	29.353453846402378	20.951665535846985
34	20.71116442118187	29.177974863493912	26.71379761558593	23.397063099738297
35	20.98962876805273	25.802478110561854	33.09909211334044	20.108801008044974
36	22.732504604051567	27.36119188394559	25.61023876449872	24.29606474750412
37	22.33409259797745	26.35254111337275	28.63558527995864	22.677781008691156
38	21.952037090885593	25.137919614875127	28.34298568705373	24.567057607185554
39	25.049675293205386	25.951503990178022	26.811330813220895	22.18748990339569
40	20.846458918936385	25.143977577461147	32.26935317114148	21.74021033246099
41	22.65536654712287	26.895536493166617	26.535491583470648	23.913605376239865
42	22.02978094407289	24.960219379018447	30.904494200510484	22.105505476398175
43	23.31063616684437	24.476592032567606	29.134559464960745	23.07821233562728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	6.5
2	6.0
3	34.0
4	62.0
5	62.0
6	112.5
7	163.0
8	161.5
9	160.0
10	246.0
11	332.0
12	332.0
13	565.5
14	799.0
15	1868.0
16	2937.0
17	3310.0
18	3683.0
19	3683.0
20	3839.0
21	3995.0
22	4904.5
23	5814.0
24	7355.5
25	8897.0
26	8897.0
27	10934.0
28	12971.0
29	15681.0
30	18391.0
31	20675.0
32	22959.0
33	22959.0
34	24954.0
35	26949.0
36	27992.0
37	29035.0
38	30884.5
39	32734.0
40	32734.0
41	33446.5
42	34159.0
43	33189.0
44	32219.0
45	32324.5
46	32430.0
47	32430.0
48	33437.0
49	34444.0
50	42063.5
51	49683.0
52	40928.5
53	32174.0
54	32174.0
55	36798.0
56	41422.0
57	34530.0
58	27638.0
59	20624.0
60	13610.0
61	13610.0
62	11943.0
63	10276.0
64	8520.0
65	6764.0
66	5514.0
67	4264.0
68	4264.0
69	3396.5
70	2529.0
71	2112.5
72	1696.0
73	1350.5
74	1005.0
75	1005.0
76	765.0
77	525.0
78	397.5
79	270.0
80	195.0
81	120.0
82	120.0
83	81.5
84	43.0
85	32.5
86	22.0
87	19.0
88	16.0
89	16.0
90	13.5
91	11.0
92	6.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	495216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.56066746516582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.14646984288225	52.776483809178934
2	7.93941449601267	9.616324823222808
3	1.8614418875704135	3.3819048947664716
4	0.8136213388177157	1.9709380537081072
5	0.4341443620269592	1.3146036170295619
6	0.2691338555809609	0.9779355558873921
7	0.2216913018847046	0.9398041249351499
8	0.14252952136867067	0.6905346358061847
9	0.13770608605657236	0.7505615237041429
>10	0.905556561039446	10.86927037991125
>50	0.08953625042801078	3.6789196328694556
>100	0.03407722953301795	3.486910894630544
>500	0.0016704524280891154	0.738697439729379
>1k	0.0016704524280891154	2.2120457706275816
>5k	0.001002271456853469	4.271738987950254
>10k+	3.34090485617823E-4	2.323325856042853
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	11483	2.31878614584343	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	8748	1.766501890084327	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	6480	1.3085199185809828	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	5885	1.1883703272915254	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	3135	0.6330570902394107	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	2875	0.5805547478272107	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1731	0.34954444121353107	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1612	0.32551452295563954	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	1580	0.31905269619721494	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	857	0.173055797874059	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	828	0.1671997673742367	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	781	0.15770895932280057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.13226551646150367	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	530	0.10702400568640753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	2.0193208620076895E-4	0.0
22	0.0	0.0	0.0	6.057962586023069E-4	0.0
23	0.0	0.0	0.0	0.0010096604310038447	0.0
24	0.0	0.0	0.0	0.0014135246034053827	0.0
25	0.0	0.0	0.0	0.0014135246034053827	0.0
26	0.0	0.0	0.0	0.0014135246034053827	0.0
27	0.0	0.0	0.0	0.0018173887758069205	0.0
28	0.0	0.0	0.0	0.005654098413621531	0.0
29	0.0	0.0	0.0	0.012519789344447675	0.0
30	0.0	0.0	0.0	0.019991276533876126	0.0
31	0.0	0.0	0.0	0.04604051565377532	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	865	0.0	31.653177	1
CGGTCGT	30	3.5965454E-4	30.833334	10
CTCGCCA	25	0.00549336	29.6	13
CCGGTCG	25	0.00549336	29.6	9
CGTCTTC	445	0.0	29.516853	37
TTATACA	1005	0.0	27.796019	2
GTATCAA	805	0.0	26.428572	1
TATACAC	1135	0.0	26.242292	3
GGTATCA	250	0.0	25.900002	1
CCGTCTT	1340	0.0	25.126863	37
GGTCGTG	45	1.3217264E-4	24.666668	11
GCCGTCT	1360	0.0	24.621325	36
TTCGTCC	40	0.0019296439	23.125	23
TCGTCCA	40	0.0019296439	23.125	24
CGCTTAA	45	0.0038227788	20.555557	1
CCGTACA	135	1.8189894E-12	20.555555	17
TGCCGTC	1650	0.0	20.293938	35
GTCCAAA	55	5.1374146E-4	20.181818	23
CGTTCGC	95	1.6713057E-7	19.473684	34
GCCCCGT	155	1.8189894E-12	19.096773	14
>>END_MODULE