FastQCFastQC Report
Fri 10 Feb 2017
ERR1631166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800658
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT154981.9356579213596816No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG122811.533863397355675No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT109281.3648773883480836TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC68380.8540475458935026No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC40060.5003384716071032RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC31430.39255212587646665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27300.34096955254303335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.3124929745284504No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG16660.20807885514164598No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA11830.14775347276864778No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9950.12427278563381619No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9780.12214953200992183No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT9430.11777812749013937No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT8570.10703696209867385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19850.033.178841
GATTACG658.731149E-1131.3076921
TCGCCGA303.5985117E-430.8333341
TTATACA22100.030.0520362
TATACAC22500.029.7644443
TGTGCGC250.005495360629.59999810
CGTCTTC4950.028.40404137
GGTATCA4650.027.4516121
TTAGTAC556.25776E-726.909093
CCGTCTT16800.026.75892837
GCCGTCT17200.026.13663136
GTATCAA13450.025.3085521
CGGATCG400.001930678423.12537
CGAAGCG400.001930678423.12529
TTACGGG1005.329639E-1022.1999993
TGCCGTC20450.021.98288535
ATGCCGT21650.020.76443534
CGAACTA656.898789E-519.92307729
GTACTAA851.2443415E-619.5882361
TATGCCG23300.019.37339233
Produced by FastQC (version 0.11.5)