##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631166.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800658
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4920065745924	31.0	31.0	34.0	28.0	34.0
2	31.504589974745773	31.0	31.0	34.0	28.0	34.0
3	30.97000217321253	31.0	31.0	34.0	27.0	34.0
4	35.09248143402052	37.0	35.0	37.0	32.0	37.0
5	35.30645668937299	37.0	35.0	37.0	33.0	37.0
6	35.5004883483335	37.0	35.0	37.0	33.0	37.0
7	35.51086106677258	37.0	35.0	37.0	33.0	37.0
8	35.532212255419914	37.0	35.0	37.0	33.0	37.0
9	37.31522947375783	39.0	37.0	39.0	34.0	39.0
10	37.13982998983336	39.0	37.0	39.0	33.0	39.0
11	37.208568452447864	39.0	37.0	39.0	33.0	39.0
12	37.14366558505629	39.0	37.0	39.0	33.0	39.0
13	37.21760352110389	39.0	37.0	39.0	34.0	39.0
14	38.3447951559842	40.0	38.0	41.0	34.0	41.0
15	38.35420741440166	40.0	38.0	41.0	34.0	41.0
16	38.35385895101279	40.0	38.0	41.0	34.0	41.0
17	38.332109340067795	40.0	38.0	41.0	34.0	41.0
18	38.3935188307617	40.0	38.0	41.0	34.0	41.0
19	38.4048257807953	40.0	38.0	41.0	34.0	41.0
20	38.352924719418276	40.0	38.0	41.0	34.0	41.0
21	38.337683505316875	40.0	38.0	41.0	34.0	41.0
22	38.27208371114758	40.0	38.0	41.0	34.0	41.0
23	38.27608292179682	40.0	38.0	41.0	34.0	41.0
24	38.23756460311394	40.0	38.0	41.0	34.0	41.0
25	38.17905272913029	40.0	38.0	41.0	33.0	41.0
26	38.09500310994207	40.0	37.0	41.0	33.0	41.0
27	37.9939737066263	40.0	37.0	41.0	33.0	41.0
28	37.98671717512346	40.0	37.0	41.0	33.0	41.0
29	37.975979756650155	40.0	37.0	41.0	33.0	41.0
30	37.818850245672934	40.0	37.0	41.0	33.0	41.0
31	37.823642553999335	40.0	37.0	41.0	33.0	41.0
32	37.7611227265574	40.0	37.0	41.0	33.0	41.0
33	37.69988809204429	40.0	37.0	41.0	33.0	41.0
34	37.67082324787862	40.0	37.0	41.0	33.0	41.0
35	37.64563896195379	40.0	37.0	41.0	33.0	41.0
36	37.59826167976839	40.0	37.0	41.0	33.0	41.0
37	37.57436883163598	40.0	37.0	41.0	32.0	41.0
38	37.53803121932211	40.0	37.0	41.0	32.0	41.0
39	37.45501325160056	40.0	37.0	41.0	32.0	41.0
40	37.36216087268222	39.0	37.0	41.0	32.0	41.0
41	37.32082487154315	39.0	36.0	41.0	31.0	41.0
42	37.27557084298165	39.0	36.0	41.0	31.0	41.0
43	36.62062203837344	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	13.0
11	14.0
12	15.0
13	9.0
14	10.0
15	7.0
16	7.0
17	5.0
18	18.0
19	37.0
20	74.0
21	149.0
22	252.0
23	533.0
24	997.0
25	1691.0
26	2673.0
27	4281.0
28	6344.0
29	8993.0
30	12773.0
31	17138.0
32	22631.0
33	29371.0
34	38744.0
35	52408.0
36	73840.0
37	109958.0
38	192466.0
39	225202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.69711911952419	22.49175053518481	15.523731730651539	24.287398614639457
2	13.451685988274646	22.06147943316622	41.56081622865193	22.926018349907203
3	14.346574942110117	28.935200797344184	34.71894366883239	21.999280591713315
4	11.35953178510675	19.555915259698896	38.18284461030802	30.90170834488633
5	12.171613847610342	38.52056683377922	35.891978847398015	13.415840471212428
6	27.043631612998308	40.52391907655953	17.931876031963707	14.500573278478452
7	23.86574542438844	33.7760941625513	22.8939197510048	19.46424066205546
8	24.47886613260593	35.36116544142443	22.43692063277954	17.7230477931901
9	23.77432062128899	15.28055674207964	19.64009602102271	41.30502661560867
10	14.197072907533553	28.95530925813518	33.784212485230896	23.063405349100368
11	30.678516919833438	23.436973089633774	25.64428257757994	20.240227412952848
12	20.282692485430733	26.93434650000375	29.203854829402815	23.579106185162704
13	28.618461315567945	20.956013678749226	27.275316052546785	23.150208953136044
14	21.554396508871456	22.67822216227153	26.839674367832455	28.92770696102456
15	23.625068381256416	29.08058122194495	23.12785234144916	24.166498055349475
16	24.411671400273274	27.850218195534172	23.71324585528403	24.02486454890852
17	20.61254618076632	30.994382120705723	25.164552155851812	23.22851954267615
18	24.017745404404877	25.910688458742687	26.10815104576486	23.96341509108758
19	24.725288450249668	27.081600383684417	26.685176442376147	21.507934723689765
20	22.3713495649828	26.32259966177819	27.43018866981907	23.875862103419937
21	24.443520204631692	27.26807201077114	25.629045110396696	22.65936267420047
22	22.095201696604544	30.672396953505743	26.428762342972906	20.80363900691681
23	23.06852613725211	27.351753183006977	26.447871625587954	23.13184905415296
24	25.82251098471507	27.56595200447632	25.937166680405365	20.674370330403242
25	22.633134247081777	28.731992935810297	26.903496873821283	21.731375943286647
26	25.08461790177579	28.697521288740013	25.73783063430329	20.480030175180914
27	22.25619428020453	29.186743903139668	27.80025928673666	20.756802529919142
28	20.805012876908744	28.716505674083066	27.71420506633294	22.76427638267525
29	25.80340170210002	26.1554871118505	26.42526521935708	21.615845966692397
30	21.630958536603643	28.02982047266124	29.599279592535137	20.739941398199978
31	23.590346939642146	28.49618688628603	25.843368829138036	22.070097344933792
32	25.406228377159785	26.65407702164969	27.136555183361683	20.803139417828838
33	20.89368994002433	29.596531852551276	28.67541447159711	20.83436373582728
34	23.390386407180095	28.156341409190937	26.689422949623935	21.763849234005033
35	23.241883550779484	26.05956600695928	30.28621458850096	20.412335853760283
36	22.647247638817074	28.20667500980444	25.71909604350422	23.42698130787427
37	21.987040659057925	27.19875402481459	28.067664346075354	22.74654097005213
38	22.01789028524039	25.410849576223555	28.83178585613333	23.73947428240272
39	23.720614794331663	26.09890864763732	27.00803589048008	23.17244066755094
40	20.804263493276782	25.26247161709494	32.02703276555033	21.906232124077945
41	22.125676630971025	26.25103352492575	26.839174778744486	24.78411506535874
42	22.728181071069045	25.279707440630084	29.929507979686708	22.062603508614163
43	22.59529037366766	24.827829110556568	29.90777585435979	22.669104661415986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	11.5
2	18.0
3	77.0
4	136.0
5	136.0
6	199.5
7	263.0
8	253.0
9	243.0
10	404.0
11	565.0
12	565.0
13	852.0
14	1139.0
15	2755.0
16	4371.0
17	5084.0
18	5797.0
19	5797.0
20	6139.5
21	6482.0
22	7974.0
23	9466.0
24	12108.0
25	14750.0
26	14750.0
27	18001.0
28	21252.0
29	25345.0
30	29438.0
31	33247.0
32	37056.0
33	37056.0
34	40421.5
35	43787.0
36	46138.5
37	48490.0
38	50918.0
39	53346.0
40	53346.0
41	54528.5
42	55711.0
43	55240.5
44	54770.0
45	55330.0
46	55890.0
47	55890.0
48	55790.0
49	55690.0
50	68069.0
51	80448.0
52	65383.0
53	50318.0
54	50318.0
55	55300.5
56	60283.0
57	50841.5
58	41400.0
59	32210.0
60	23020.0
61	23020.0
62	20043.0
63	17066.0
64	14145.5
65	11225.0
66	9336.5
67	7448.0
68	7448.0
69	5990.0
70	4532.0
71	3762.5
72	2993.0
73	2291.5
74	1590.0
75	1590.0
76	1212.0
77	834.0
78	669.0
79	504.0
80	343.0
81	182.0
82	182.0
83	132.5
84	83.0
85	61.5
86	40.0
87	28.0
88	16.0
89	16.0
90	11.5
91	7.0
92	5.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	800658.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.596157197042736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.91682436656849	44.72343820199714
2	11.074525068726667	12.092530230695832
3	2.9185285788504753	4.780213352249469
4	1.1534594603857784	2.518978160785522
5	0.600521253354411	1.6393076374151276
6	0.39698809491565107	1.3004414661221644
7	0.2765746940772738	1.0569940832196791
8	0.191461940738114	0.8362468971031163
9	0.16280216924147678	0.7999535541534498
>10	1.1201353475858356	12.16060971972033
>50	0.12797121367687447	4.753098262829223
>100	0.05654497921753224	5.129524462015604
>500	0.001373562248009687	0.6126782707681664
>1k	0.001373562248009687	1.903527144797873
>5k	2.289270413349478E-4	0.8546499419650595
>10k+	6.867811240048435E-4	4.837808614162264
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	15498	1.9356579213596816	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	12281	1.533863397355675	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	10928	1.3648773883480836	TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	6838	0.8540475458935026	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	4006	0.5003384716071032	RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	3143	0.39255212587646665	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2730	0.34096955254303335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2502	0.3124929745284504	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	1666	0.20807885514164598	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1183	0.14775347276864778	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	995	0.12427278563381619	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	978	0.12214953200992183	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	943	0.11777812749013937	No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	857	0.10703696209867385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2489727199378511E-4	0.0
11	0.0	0.0	0.0	2.4979454398757023E-4	0.0
12	0.0	0.0	0.0	3.746918159813553E-4	0.0
13	0.0	0.0	0.0	3.746918159813553E-4	0.0
14	0.0	0.0	0.0	3.746918159813553E-4	0.0
15	0.0	0.0	0.0	4.995890879751405E-4	0.0
16	0.0	0.0	0.0	7.493836319627106E-4	0.0
17	0.0	0.0	0.0	8.742809039564958E-4	0.0
18	0.0	0.0	0.0	9.99178175950281E-4	0.0
19	0.0	0.0	0.0	9.99178175950281E-4	0.0
20	0.0	0.0	0.0	9.99178175950281E-4	0.0
21	0.0	0.0	0.0	0.0014987672639254213	0.0
22	0.0	0.0	0.0	0.0017485618079129915	0.0
23	0.0	0.0	0.0	0.002123253623894347	0.0
24	0.0	0.0	0.0	0.0028726372558570577	0.0
25	0.0	0.0	0.0	0.0031224317998446278	0.0
26	0.0	0.0	0.0	0.003372226343832198	0.0
27	0.0	0.0	0.0	0.004371404519782479	0.0
28	0.0	0.0	0.0	0.008742809039564958	0.0
29	0.0	0.0	0.0	0.02048315260698076	0.0
30	0.0	0.0	0.0	0.0352210307022474	0.0
31	0.0	0.0	0.0	0.0746885686522835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1985	0.0	33.17884	1
GATTACG	65	8.731149E-11	31.307692	1
TCGCCGA	30	3.5985117E-4	30.833334	1
TTATACA	2210	0.0	30.052036	2
TATACAC	2250	0.0	29.764444	3
TGTGCGC	25	0.0054953606	29.599998	10
CGTCTTC	495	0.0	28.404041	37
GGTATCA	465	0.0	27.451612	1
TTAGTAC	55	6.25776E-7	26.90909	3
CCGTCTT	1680	0.0	26.758928	37
GCCGTCT	1720	0.0	26.136631	36
GTATCAA	1345	0.0	25.308552	1
CGGATCG	40	0.0019306784	23.125	37
CGAAGCG	40	0.0019306784	23.125	29
TTACGGG	100	5.329639E-10	22.199999	3
TGCCGTC	2045	0.0	21.982885	35
ATGCCGT	2165	0.0	20.764435	34
CGAACTA	65	6.898789E-5	19.923077	29
GTACTAA	85	1.2443415E-6	19.588236	1
TATGCCG	2330	0.0	19.373392	33
>>END_MODULE