FastQCFastQC Report
Fri 10 Feb 2017
ERR1631165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386495
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT54550.3934381299608004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42620.30739382399503784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36280.26166700925715564No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG33410.2409673312922153No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT22260.16054872177685459RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC16950.12225071132604157No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14960.10789797294617001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14520.1047245031536356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7700.027.149351
GATTACG1200.024.6666661
GTATCAA24000.024.281251
TATACAC9400.023.4202143
GCCGTCT4700.020.86170236
CCGTCTT4850.020.21649637
TTATACA10850.019.267282
CTTATAC11050.019.0859741
CGTAGGC1002.875986E-718.49999818
AACGCGC1002.875986E-718.49999832
TAATACG1451.546141E-1017.8620684
GAACGCG1054.7990216E-717.61904731
TTACGGG1705.456968E-1217.4117643
ATACGCC752.0676535E-417.2666666
GGTACGC650.001580156617.07692330
ATGCCGT6000.016.95833434
TGCCGTC6050.016.81818235
TTAACGG1708.54925E-1116.3235335
TACACAT14150.016.0812725
GTACTAG700.002592854715.8571431
Produced by FastQC (version 0.11.5)