##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1386495
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.497556067638182	31.0	31.0	34.0	28.0	34.0
2	31.479533644189125	31.0	31.0	34.0	28.0	34.0
3	30.93987645105103	31.0	31.0	34.0	27.0	34.0
4	35.083381476312574	37.0	35.0	37.0	32.0	37.0
5	35.30006310877428	37.0	35.0	37.0	33.0	37.0
6	35.50256510120844	37.0	35.0	37.0	33.0	37.0
7	35.52030623983498	37.0	35.0	37.0	33.0	37.0
8	35.539328306268686	37.0	35.0	37.0	33.0	37.0
9	37.30598956361184	39.0	37.0	39.0	34.0	39.0
10	37.13728646695444	39.0	37.0	39.0	33.0	39.0
11	37.20779591704262	39.0	37.0	39.0	33.0	39.0
12	37.14500160476597	39.0	37.0	39.0	33.0	39.0
13	37.20330689977245	39.0	37.0	39.0	33.0	39.0
14	38.38735660784929	40.0	38.0	41.0	34.0	41.0
15	38.38864114187213	40.0	38.0	41.0	34.0	41.0
16	38.39011175662372	40.0	38.0	41.0	34.0	41.0
17	38.37827832051324	40.0	38.0	41.0	34.0	41.0
18	38.403451148399384	40.0	38.0	41.0	34.0	41.0
19	38.44354649674178	40.0	38.0	41.0	34.0	41.0
20	38.42995106365331	40.0	38.0	41.0	34.0	41.0
21	38.38259207570168	40.0	38.0	41.0	34.0	41.0
22	38.35173296694182	40.0	38.0	41.0	34.0	41.0
23	38.31073606468109	40.0	38.0	41.0	34.0	41.0
24	38.29328270206528	40.0	38.0	41.0	34.0	41.0
25	38.2672811658174	40.0	38.0	41.0	34.0	41.0
26	38.1689923151544	40.0	38.0	41.0	34.0	41.0
27	38.118843558757874	40.0	38.0	41.0	33.0	41.0
28	38.082887424765325	40.0	37.0	41.0	33.0	41.0
29	38.00982982268238	40.0	37.0	41.0	33.0	41.0
30	37.93073902177794	40.0	37.0	41.0	33.0	41.0
31	37.90010133466042	40.0	37.0	41.0	33.0	41.0
32	37.83663554502541	40.0	37.0	41.0	33.0	41.0
33	37.81905380113163	40.0	37.0	41.0	33.0	41.0
34	37.75861290520341	40.0	37.0	41.0	33.0	41.0
35	37.72041009884637	40.0	37.0	41.0	33.0	41.0
36	37.693413247072655	40.0	37.0	41.0	33.0	41.0
37	37.63817900533359	40.0	37.0	41.0	33.0	41.0
38	37.59350520557233	40.0	37.0	41.0	32.0	41.0
39	37.538099307967215	40.0	37.0	41.0	32.0	41.0
40	37.491039635916465	40.0	37.0	41.0	32.0	41.0
41	37.42175269294155	40.0	37.0	41.0	32.0	41.0
42	37.35958514094894	39.0	36.0	41.0	32.0	41.0
43	36.779368840132854	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	2.0
10	2.0
11	8.0
12	8.0
13	7.0
14	5.0
15	8.0
16	7.0
17	9.0
18	14.0
19	44.0
20	95.0
21	192.0
22	373.0
23	800.0
24	1545.0
25	2638.0
26	4438.0
27	7000.0
28	10546.0
29	15097.0
30	21385.0
31	28676.0
32	38129.0
33	49712.0
34	66435.0
35	90220.0
36	126062.0
37	189568.0
38	335560.0
39	397905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.745332655364784	21.15023855116679	14.546752783096945	23.557676010371477
2	14.597023429583231	22.397628552573217	40.01601159758961	22.98933642025395
3	15.489273311479668	27.813299002160125	32.88695595728798	23.81047172907223
4	12.326189420084457	18.60360116697139	39.94215630059971	29.12805311234444
5	12.855149135049171	38.130754167883765	35.986642577145965	13.027454119921094
6	28.477924550755684	40.34857680698452	18.003887500495853	13.169611141763946
7	25.685487506265797	32.64844085265363	23.878268583730918	17.787803057349645
8	24.658870028380917	35.83878773453925	21.23570586262482	18.26663637445501
9	25.41343459586944	15.517401793731675	20.66440917565516	38.40475443474373
10	14.581083956307092	28.839267361223804	34.24166693713284	22.33798174533626
11	32.766796851052476	23.84162943248984	25.11000760911507	18.281566107342613
12	22.052080966754296	25.999516767099774	31.067475901463766	20.880926364682168
13	28.13461281865423	22.215226163815956	26.51816270523875	23.131998312291067
14	21.7651704477838	21.300112874550575	28.119683085766628	28.815033591899
15	23.15825156239294	29.19246012427019	24.481876963133654	23.16741135020321
16	22.928175002434195	28.524733230195565	25.247692923522987	23.299398843847253
17	21.84385807377596	28.319755931323225	26.7687946945355	23.067591300365308
18	23.145124937342	27.21567694077512	27.706410769602485	21.932787352280393
19	24.123130627950335	26.826349896681922	27.318958957659422	21.731560517708324
20	23.608884272932826	26.690684063051073	27.37658628411931	22.323845379896788
21	23.271703107476046	26.90309016621048	27.51030476128655	22.31490196502692
22	22.32059978579079	27.775794359157445	27.41445154868932	22.489154306362447
23	22.70357989029892	26.9487448566349	27.79180595674705	22.555869296319138
24	22.905383719378722	27.43601671841586	27.052675992340397	22.60592356986502
25	22.673864673150643	27.587477776695913	27.544924431750566	22.19373311840288
26	23.112813244908924	27.12335781953776	27.307707564758616	22.4561213707947
27	22.476676800132708	27.668257007778607	27.210988860399787	22.6440773316889
28	22.335241021424526	27.09421959689721	27.70871874763342	22.86182063404484
29	23.129329712692797	27.196780370646845	27.101215655303477	22.572674261356877
30	22.614434238854088	27.226784085048987	27.602046888016186	22.55673478808074
31	23.380971442378083	26.720543528826283	27.485493997454014	22.41299103134162
32	22.176856029051674	27.683980108114348	27.586756533561246	22.552407329272732
33	22.247465731935563	27.472006750835742	27.813875996667857	22.466651520560838
34	22.894853569612582	26.754369831842162	27.58848751708445	22.762289081460807
35	22.31490196502692	27.536197389821094	28.051597733854074	22.097302911297913
36	23.22777940057483	26.880082510214603	26.918596893605816	22.973541195604742
37	22.69442010248865	27.031976314375456	27.59541145117725	22.678192131958642
38	22.650712768527832	26.79360545836804	27.602479633896987	22.95320213920714
39	22.769573637120942	26.675826454476937	27.69501512807475	22.85958478032737
40	22.43635930890483	26.922347357906084	28.33721001518217	22.304083318006917
41	22.673431927269842	26.387473449237103	27.30936642396835	23.629728199524703
42	22.572457888416476	26.775718628628304	27.779472699144247	22.872350783810976
43	22.81977215929376	26.136336589746083	28.069268190653414	22.974623060306744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	18.5
2	33.0
3	119.5
4	206.0
5	206.0
6	276.0
7	346.0
8	350.0
9	354.0
10	613.0
11	872.0
12	872.0
13	1271.5
14	1671.0
15	3966.0
16	6261.0
17	7427.0
18	8593.0
19	8593.0
20	8864.5
21	9136.0
22	11256.0
23	13376.0
24	17902.5
25	22429.0
26	22429.0
27	28549.5
28	34670.0
29	41330.0
30	47990.0
31	54721.5
32	61453.0
33	61453.0
34	68306.0
35	75159.0
36	81258.0
37	87357.0
38	93190.0
39	99023.0
40	99023.0
41	103133.0
42	107243.0
43	109022.5
44	110802.0
45	109943.0
46	109084.0
47	109084.0
48	106587.0
49	104090.0
50	106010.0
51	107930.0
52	98985.0
53	90040.0
54	90040.0
55	86696.5
56	83353.0
57	73449.0
58	63545.0
59	55660.5
60	47776.0
61	47776.0
62	41696.0
63	35616.0
64	29589.0
65	23562.0
66	19067.0
67	14572.0
68	14572.0
69	11897.5
70	9223.0
71	7128.0
72	5033.0
73	3879.5
74	2726.0
75	2726.0
76	2084.5
77	1443.0
78	1102.0
79	761.0
80	587.5
81	414.0
82	414.0
83	313.5
84	213.0
85	151.0
86	89.0
87	58.5
88	28.0
89	28.0
90	21.5
91	15.0
92	9.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1386495.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.16330092887269
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8518834065607	46.44449124313045
2	12.450019296929755	14.482684378751944
3	3.3982533711164433	5.929609003704053
4	1.3727983862571247	3.193859426181758
5	0.7097741493125077	2.0641403718999003
6	0.43835574796612004	1.5297730369712708
7	0.27321299435104107	1.1123678725682595
8	0.21556217324403418	1.003024604101964
9	0.15545134656728296	0.8137407105172224
>10	0.969948178238245	10.891129224885198
>50	0.10443076537463461	4.215482539057809
>100	0.05694580469289796	5.729284080531324
>500	0.0023675262368720025	0.883256936330554
>1k	8.722465083212639E-4	1.3118036061034732
>5k	1.2460664404589485E-4	0.39535296526488656
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	5455	0.3934381299608004	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4262	0.30739382399503784	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3628	0.26166700925715564	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	3341	0.2409673312922153	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	2226	0.16054872177685459	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	1695	0.12225071132604157	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1496	0.10789797294617001	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1452	0.1047245031536356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.212431346669119E-5	0.0
4	0.0	0.0	0.0	1.4424862693338238E-4	0.0
5	0.0	0.0	0.0	2.1637294040007356E-4	0.0
6	0.0	0.0	0.0	2.1637294040007356E-4	0.0
7	0.0	0.0	0.0	2.8849725386676477E-4	0.0
8	0.0	0.0	0.0	2.8849725386676477E-4	1.4424862693338238E-4
9	0.0	0.0	0.0	2.8849725386676477E-4	1.4424862693338238E-4
10	0.0	0.0	0.0	2.8849725386676477E-4	1.4424862693338238E-4
11	0.0	0.0	0.0	2.8849725386676477E-4	1.4424862693338238E-4
12	0.0	0.0	0.0	3.606215673334559E-4	1.4424862693338238E-4
13	0.0	0.0	0.0	3.606215673334559E-4	1.4424862693338238E-4
14	0.0	0.0	0.0	4.327458808001471E-4	1.4424862693338238E-4
15	0.0	0.0	0.0	5.048701942668383E-4	1.4424862693338238E-4
16	0.0	0.0	0.0	7.212431346669118E-4	1.4424862693338238E-4
17	0.0	0.0	0.0	7.212431346669118E-4	1.4424862693338238E-4
18	0.0	0.0	0.0	7.212431346669118E-4	2.1637294040007356E-4
19	0.0	0.0	0.0	7.93367448133603E-4	2.1637294040007356E-4
20	0.0	0.0	0.0	0.0010818647020003679	2.1637294040007356E-4
21	0.0	0.0	0.0	0.001586734896267206	2.8849725386676477E-4
22	0.0	0.0	0.0	0.0017309835232005885	2.8849725386676477E-4
23	0.0	0.0	0.0	0.002307978030934118	2.8849725386676477E-4
24	0.0	0.0	0.0	0.0030292211656010298	2.8849725386676477E-4
25	0.0	0.0	0.0	0.003173469792534412	2.8849725386676477E-4
26	0.0	0.0	0.0	0.0033177184194677946	2.8849725386676477E-4
27	0.0	0.0	0.0	0.004111085867601397	3.606215673334559E-4
28	0.0	0.0	0.0	0.010458025452670222	3.606215673334559E-4
29	0.0	0.0	0.0	0.02603687716147552	3.606215673334559E-4
30	0.0	0.0	0.0	0.048251165709216406	3.606215673334559E-4
31	0.0	0.0	0.0	0.09390585613363193	3.606215673334559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	770	0.0	27.14935	1
GATTACG	120	0.0	24.666666	1
GTATCAA	2400	0.0	24.28125	1
TATACAC	940	0.0	23.420214	3
GCCGTCT	470	0.0	20.861702	36
CCGTCTT	485	0.0	20.216496	37
TTATACA	1085	0.0	19.26728	2
CTTATAC	1105	0.0	19.085974	1
CGTAGGC	100	2.875986E-7	18.499998	18
AACGCGC	100	2.875986E-7	18.499998	32
TAATACG	145	1.546141E-10	17.862068	4
GAACGCG	105	4.7990216E-7	17.619047	31
TTACGGG	170	5.456968E-12	17.411764	3
ATACGCC	75	2.0676535E-4	17.266666	6
GGTACGC	65	0.0015801566	17.076923	30
ATGCCGT	600	0.0	16.958334	34
TGCCGTC	605	0.0	16.818182	35
TTAACGG	170	8.54925E-11	16.32353	35
TACACAT	1415	0.0	16.081272	5
GTACTAG	70	0.0025928547	15.857143	1
>>END_MODULE