FastQCFastQC Report
Fri 10 Feb 2017
ERR1631164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684859
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT111011.6209175903361128No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG84051.2272599177348913No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT66430.969980682155013RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC43960.6418839498349295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24900.363578488418784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21770.3178756503163425No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC20670.30181395002474964RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC19850.2898406825346531No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG11560.16879386851892142No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT9620.14046686982283946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8590.12542727773162066No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA7880.11506018027068346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG200.00184141737.05
CTTATAC12800.031.0742191
CCGTCTT9700.028.7989737
CGTCTTC2350.028.34042437
TTATACA14050.028.3096082
GCCGTCT10050.027.79601936
TATACAC14750.027.7186453
GATTACG358.865719E-426.4285721
GGTATCA4600.025.3369581
ACGGGTA601.3360877E-624.66666815
GTATCAA12700.024.4724391
TGCCGTC12050.023.336135
ATGCCGT12450.022.28915834
TATGCCG13200.021.16287833
GAGTGCG450.003824252620.5555557
CGGGTAA555.140177E-420.18181816
TTAACGG555.140177E-420.18181835
TTTGCCG555.140177E-420.1818189
GCCGAGT701.2182842E-418.512
CCGAGTT609.231618E-418.513