Basic Statistics
Measure | Value |
---|---|
Filename | ERR1631164.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 684859 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 44 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT | 11101 | 1.6209175903361128 | No Hit |
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG | 8405 | 1.2272599177348913 | No Hit |
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT | 6643 | 0.969980682155013 | RNA PCR Primer, Index 33 (95% over 22bp) |
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC | 4396 | 0.6418839498349295 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT | 2490 | 0.363578488418784 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2177 | 0.3178756503163425 | No Hit |
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC | 2067 | 0.30181395002474964 | RNA PCR Primer, Index 33 (95% over 23bp) |
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC | 1985 | 0.2898406825346531 | No Hit |
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG | 1156 | 0.16879386851892142 | No Hit |
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT | 962 | 0.14046686982283946 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 859 | 0.12542727773162066 | No Hit |
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA | 788 | 0.11506018027068346 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACTACCG | 20 | 0.001841417 | 37.0 | 5 |
CTTATAC | 1280 | 0.0 | 31.074219 | 1 |
CCGTCTT | 970 | 0.0 | 28.79897 | 37 |
CGTCTTC | 235 | 0.0 | 28.340424 | 37 |
TTATACA | 1405 | 0.0 | 28.309608 | 2 |
GCCGTCT | 1005 | 0.0 | 27.796019 | 36 |
TATACAC | 1475 | 0.0 | 27.718645 | 3 |
GATTACG | 35 | 8.865719E-4 | 26.428572 | 1 |
GGTATCA | 460 | 0.0 | 25.336958 | 1 |
ACGGGTA | 60 | 1.3360877E-6 | 24.666668 | 15 |
GTATCAA | 1270 | 0.0 | 24.472439 | 1 |
TGCCGTC | 1205 | 0.0 | 23.3361 | 35 |
ATGCCGT | 1245 | 0.0 | 22.289158 | 34 |
TATGCCG | 1320 | 0.0 | 21.162878 | 33 |
GAGTGCG | 45 | 0.0038242526 | 20.555555 | 7 |
CGGGTAA | 55 | 5.140177E-4 | 20.181818 | 16 |
TTAACGG | 55 | 5.140177E-4 | 20.181818 | 35 |
TTTGCCG | 55 | 5.140177E-4 | 20.181818 | 9 |
GCCGAGT | 70 | 1.2182842E-4 | 18.5 | 12 |
CCGAGTT | 60 | 9.231618E-4 | 18.5 | 13 |