##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631164.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684859
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.474532714033106	31.0	31.0	34.0	28.0	34.0
2	31.47514451879876	31.0	31.0	34.0	28.0	34.0
3	30.94865220432235	31.0	31.0	34.0	27.0	34.0
4	35.07915789965526	37.0	35.0	37.0	32.0	37.0
5	35.28937197291705	37.0	35.0	37.0	33.0	37.0
6	35.49031114433774	37.0	35.0	37.0	33.0	37.0
7	35.502084370651474	37.0	35.0	37.0	33.0	37.0
8	35.52227392791801	37.0	35.0	37.0	33.0	37.0
9	37.29750649403746	39.0	37.0	39.0	34.0	39.0
10	37.122236839991885	39.0	37.0	39.0	33.0	39.0
11	37.19183072720078	39.0	37.0	39.0	33.0	39.0
12	37.129556594861135	39.0	37.0	39.0	33.0	39.0
13	37.19386764282867	39.0	37.0	39.0	33.0	39.0
14	38.34551053574531	40.0	38.0	41.0	34.0	41.0
15	38.3512606244497	40.0	38.0	41.0	34.0	41.0
16	38.3407095475127	40.0	38.0	41.0	34.0	41.0
17	38.3288092877512	40.0	38.0	41.0	34.0	41.0
18	38.362797305722786	40.0	38.0	41.0	34.0	41.0
19	38.40069707779266	40.0	38.0	41.0	34.0	41.0
20	38.385575717045405	40.0	38.0	41.0	34.0	41.0
21	38.34538496245212	40.0	38.0	41.0	34.0	41.0
22	38.274186365368635	40.0	38.0	41.0	34.0	41.0
23	38.27101052917462	40.0	38.0	41.0	34.0	41.0
24	38.23098623220254	40.0	38.0	41.0	34.0	41.0
25	38.2013188116094	40.0	38.0	41.0	34.0	41.0
26	38.08996012317864	40.0	37.0	41.0	33.0	41.0
27	38.01982743893269	40.0	37.0	41.0	33.0	41.0
28	38.0125821519466	40.0	37.0	41.0	33.0	41.0
29	37.93196555787396	40.0	37.0	41.0	33.0	41.0
30	37.85352459411353	40.0	37.0	41.0	33.0	41.0
31	37.841431593948535	40.0	37.0	41.0	33.0	41.0
32	37.74327708331204	40.0	37.0	41.0	33.0	41.0
33	37.73043794416077	40.0	37.0	41.0	33.0	41.0
34	37.66618530237611	40.0	37.0	41.0	33.0	41.0
35	37.635891475471595	40.0	37.0	41.0	33.0	41.0
36	37.61217564491377	40.0	37.0	41.0	33.0	41.0
37	37.55566912313338	40.0	37.0	41.0	32.0	41.0
38	37.51717944861643	40.0	37.0	41.0	32.0	41.0
39	37.44957867239826	40.0	37.0	41.0	32.0	41.0
40	37.360186549348114	39.0	37.0	41.0	32.0	41.0
41	37.30901543237367	39.0	36.0	41.0	31.0	41.0
42	37.262877468208785	39.0	36.0	41.0	31.0	41.0
43	36.612550904638766	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	7.0
12	3.0
13	5.0
14	5.0
15	5.0
16	4.0
17	10.0
18	14.0
19	25.0
20	57.0
21	121.0
22	219.0
23	459.0
24	796.0
25	1409.0
26	2196.0
27	3568.0
28	5478.0
29	7837.0
30	11053.0
31	14474.0
32	19406.0
33	25390.0
34	33607.0
35	45216.0
36	63164.0
37	94438.0
38	164183.0
39	191703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.72826377400311	22.138717604645628	15.066458935342894	24.06655968600836
2	13.772908000040884	22.215229704216487	41.30266229983106	22.70919999591157
3	14.583001756565949	28.61596328587344	34.2788807623175	22.522154195243107
4	11.444545519588702	19.50927125145468	38.98904738055571	30.05713584840091
5	12.026709147430346	38.54997890076643	36.41786119478608	13.005450757017137
6	27.017678091402757	41.02917534850239	17.915950582528666	14.037195977566185
7	24.177531433477547	33.50748110194945	23.321588823392844	18.993398641180157
8	24.16482808870147	35.7778754458947	22.40972229320196	17.647574172201868
9	24.26776898602486	15.30738443971679	20.118155707963243	40.306690866295106
10	14.030917312906743	29.20425956291733	34.37510494860986	22.389718175566063
11	30.87657459418654	23.686189420012003	25.706313270322795	19.73092271547866
12	20.627457622663936	26.771642046026994	30.027494710590062	22.573405620719008
13	28.249318472853535	21.568381228836884	27.332049370746386	22.85025092756319
14	21.484276325491816	22.33583847186063	27.587138374468324	28.592746828179234
15	23.268585212430587	29.098836402821604	23.759051133152955	23.873527251594854
16	23.637420257308438	28.31473339767748	24.40195719118826	23.645889153825824
17	20.575622135359247	30.4481652427726	25.95672977941445	23.019482842453705
18	23.27442583071844	26.881445669838612	26.840561341823644	23.003567157619305
19	23.427304014402964	28.315025428591873	26.79296030277765	21.464710254227512
20	22.8736134007146	27.20019741289813	26.710899615833334	23.21528957055394
21	24.191840948282785	26.986576799019947	26.273291290616026	22.548290962081246
22	21.04068136652946	31.02112989681088	26.840561341823644	21.097627394836017
23	23.71539251145126	26.357688224875485	27.111712045837173	22.815207217836083
24	23.443365714694558	28.74810727463609	26.545902149201517	21.262624861467835
25	21.750316488503472	28.85864097573369	26.531154588024684	22.859887947738148
26	23.557549802222063	29.22689195878276	26.644900629180608	20.570657609814575
27	21.332858296379253	30.91410056668599	26.37521007973904	21.377831057195714
28	22.880184096288435	26.73338599624156	27.86179344945456	22.524636458015447
29	21.974012168928205	29.714291554904005	26.575105240640774	21.73659103552702
30	22.332772147259508	28.418550387744045	27.79389626185828	21.454781203138165
31	24.84891050566613	26.854286794800096	26.487203935408605	21.80959876412517
32	21.547939064829404	28.44089075269508	27.5287321915898	22.482437990885714
33	22.53733980279152	27.373955806961725	28.889304221744915	21.199400168501835
34	23.01013785319314	27.805723513891177	27.23334292168169	21.95079571123399
35	21.10142379672312	26.751637928391098	29.895204706370215	22.251733568515565
36	22.42972641083785	28.131191967981728	26.56838852960974	22.870693091570672
37	21.936486196428753	27.52595789790307	28.24245574636531	22.295100159302862
38	21.81543938241302	26.0376223427012	28.992245119068304	23.154693155817473
39	23.368459785152858	26.307604923057156	27.35278356566826	22.97115172612173
40	20.763251997856493	26.210942690393207	31.367040514908908	21.658764796841393
41	21.995330425678862	26.465593647743553	27.216113097732524	24.322962828845064
42	22.589029274639014	25.902119998423036	29.342682216339423	22.16616851059853
43	22.360807115041197	25.26067409495969	29.874032465076755	22.504486324922357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	17.5
2	29.0
3	84.5
4	140.0
5	140.0
6	183.0
7	226.0
8	232.5
9	239.0
10	382.5
11	526.0
12	526.0
13	800.0
14	1074.0
15	2505.5
16	3937.0
17	4561.0
18	5185.0
19	5185.0
20	5565.5
21	5946.0
22	7274.0
23	8602.0
24	11226.0
25	13850.0
26	13850.0
27	16825.0
28	19800.0
29	23061.0
30	26322.0
31	30042.0
32	33762.0
33	33762.0
34	36652.5
35	39543.0
36	41797.5
37	44052.0
38	45909.0
39	47766.0
40	47766.0
41	47826.5
42	47887.0
43	47866.5
44	47846.0
45	47205.0
46	46564.0
47	46564.0
48	45809.5
49	45055.0
50	53726.0
51	62397.0
52	51833.0
53	41269.0
54	41269.0
55	44417.5
56	47566.0
57	40427.0
58	33288.0
59	26844.0
60	20400.0
61	20400.0
62	17906.0
63	15412.0
64	12771.5
65	10131.0
66	8343.0
67	6555.0
68	6555.0
69	5208.0
70	3861.0
71	3230.5
72	2600.0
73	2077.0
74	1554.0
75	1554.0
76	1170.0
77	786.0
78	594.5
79	403.0
80	282.5
81	162.0
82	162.0
83	113.5
84	65.0
85	51.0
86	37.0
87	23.5
88	10.0
89	10.0
90	8.0
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	684859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.50014377376809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.70149945528392	51.84953038672185
2	9.004332174467415	10.895267822840887
3	2.0995501263191625	3.810692535076268
4	0.8479802984018974	2.0521171988255023
5	0.46936092922530764	1.419820184996025
6	0.3233254504377548	1.1736741742321497
7	0.2118514128404707	0.8971928654867065
8	0.16088435432410744	0.7786821254046677
9	0.1275227583076409	0.6943630690835778
>10	0.9034570897732834	10.80399597667451
>50	0.0968563589040768	4.01872689653366
>100	0.04975654250363068	5.057413128239652
>500	0.0014492196845566116	0.6419490846950853
>1k	0.0014492196845566116	2.0854212127665743
>5k	4.830732281855372E-4	2.198964221828282
>10k+	2.415366140927686E-4	1.6221891165946145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	11101	1.6209175903361128	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	8405	1.2272599177348913	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	6643	0.969980682155013	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	4396	0.6418839498349295	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2490	0.363578488418784	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2177	0.3178756503163425	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	2067	0.30181395002474964	RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	1985	0.2898406825346531	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	1156	0.16879386851892142	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	962	0.14046686982283946	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	859	0.12542727773162066	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	788	0.11506018027068346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.460154571962988E-4	0.0
11	0.0	0.0	0.0	1.460154571962988E-4	0.0
12	0.0	0.0	0.0	1.460154571962988E-4	0.0
13	0.0	0.0	0.0	1.460154571962988E-4	0.0
14	0.0	0.0	0.0	1.460154571962988E-4	0.0
15	0.0	0.0	0.0	1.460154571962988E-4	0.0
16	0.0	0.0	0.0	1.460154571962988E-4	0.0
17	0.0	0.0	0.0	2.920309143925976E-4	0.0
18	0.0	0.0	0.0	4.380463715888964E-4	0.0
19	0.0	0.0	0.0	4.380463715888964E-4	0.0
20	0.0	0.0	0.0	8.760927431777928E-4	0.0
21	0.0	0.0	0.0	0.0013141391147666892	0.0
22	0.0	0.0	0.0	0.0018982009435518845	0.0
23	0.0	0.0	0.0	0.0023362473151407807	0.0
24	0.0	0.0	0.0	0.002920309143925976	0.0
25	0.0	0.0	0.0	0.002920309143925976	0.0
26	0.0	0.0	0.0	0.0033583555155148725	0.0
27	0.0	0.0	0.0	0.003942417344300068	0.0
28	0.0	0.0	0.0	0.010075066546544617	0.0
29	0.0	0.0	0.0	0.021610287665052224	0.0
30	0.0	0.0	0.0	0.039862219814589574	0.0
31	0.0	0.0	0.0	0.07972443962917915	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCG	20	0.001841417	37.0	5
CTTATAC	1280	0.0	31.074219	1
CCGTCTT	970	0.0	28.79897	37
CGTCTTC	235	0.0	28.340424	37
TTATACA	1405	0.0	28.309608	2
GCCGTCT	1005	0.0	27.796019	36
TATACAC	1475	0.0	27.718645	3
GATTACG	35	8.865719E-4	26.428572	1
GGTATCA	460	0.0	25.336958	1
ACGGGTA	60	1.3360877E-6	24.666668	15
GTATCAA	1270	0.0	24.472439	1
TGCCGTC	1205	0.0	23.3361	35
ATGCCGT	1245	0.0	22.289158	34
TATGCCG	1320	0.0	21.162878	33
GAGTGCG	45	0.0038242526	20.555555	7
CGGGTAA	55	5.140177E-4	20.181818	16
TTAACGG	55	5.140177E-4	20.181818	35
TTTGCCG	55	5.140177E-4	20.181818	9
GCCGAGT	70	1.2182842E-4	18.5	12
CCGAGTT	60	9.231618E-4	18.5	13
>>END_MODULE