FastQCFastQC Report
Fri 10 Feb 2017
ERR1631163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027363
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT64980.6324930915362925No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG51650.5027434314842952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43470.42312210971195185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.37406447380331975No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT37980.369684327740049TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC29830.29035501570525707No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14970.1457128590381394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14390.14006733744547933No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC13790.1342271426944517No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC13140.12790026504750512Illumina Paired End PCR Primer 2 (95% over 23bp)
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT10980.10687556394380565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG200.00184184537.036
GGTATCA7250.029.8551731
GATTACG652.9904186E-928.4615361
CTTATAC9700.027.2731951
GACGTAT358.8687515E-426.42857228
CGTCTTC2300.024.93478437
TATACAC11150.024.5560533
TTATACA10700.024.5514032
GTATCAA20900.024.2535881
GCCGTCT6450.023.80620236
CTAGTAC802.725028E-823.1253
ATGCGCT400.001931048223.12531
CCGTCTT6650.023.09022537
TTACGGG855.1793904E-821.7647063
GCAACGC705.101052E-621.1428593
TAACACG450.003825535520.5555554
CGTACTG450.003825535520.55555514
CGTGCGA656.901221E-519.92307532
TGCCGTC8300.018.7228935
ATGCCGT8250.018.61212234
Produced by FastQC (version 0.11.5)