##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631163.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1027363
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31794701580649	31.0	31.0	34.0	28.0	34.0
2	31.304681013429526	31.0	31.0	34.0	28.0	34.0
3	30.71395894148417	31.0	31.0	34.0	26.0	34.0
4	34.91110444896302	37.0	35.0	37.0	32.0	37.0
5	35.142592248309505	37.0	35.0	37.0	32.0	37.0
6	35.358664853610655	37.0	35.0	37.0	33.0	37.0
7	35.37358265773636	37.0	35.0	37.0	33.0	37.0
8	35.396002192019765	37.0	35.0	37.0	33.0	37.0
9	37.13857614105238	39.0	37.0	39.0	33.0	39.0
10	36.95003810727075	39.0	37.0	39.0	33.0	39.0
11	37.02985118210409	39.0	37.0	39.0	33.0	39.0
12	36.96197546534185	39.0	37.0	39.0	33.0	39.0
13	37.03395975911143	39.0	37.0	39.0	33.0	39.0
14	38.14343323635366	40.0	37.0	41.0	33.0	41.0
15	38.14638058797134	40.0	37.0	41.0	33.0	41.0
16	38.14692567281477	40.0	37.0	41.0	33.0	41.0
17	38.148590128318816	40.0	37.0	41.0	33.0	41.0
18	38.17171729953288	40.0	38.0	41.0	33.0	41.0
19	38.20478058875003	40.0	38.0	41.0	33.0	41.0
20	38.1972954058108	40.0	38.0	41.0	33.0	41.0
21	38.15128732492799	40.0	37.0	41.0	33.0	41.0
22	38.10109377114029	40.0	37.0	41.0	33.0	41.0
23	38.070048269209614	40.0	37.0	41.0	33.0	41.0
24	38.04562846822399	40.0	37.0	41.0	33.0	41.0
25	38.00682426756658	40.0	37.0	41.0	33.0	41.0
26	37.89954086335599	40.0	37.0	41.0	33.0	41.0
27	37.834698154401124	40.0	37.0	41.0	33.0	41.0
28	37.795908554230586	40.0	37.0	41.0	33.0	41.0
29	37.728715166888435	40.0	37.0	41.0	33.0	41.0
30	37.632202055164534	39.0	37.0	41.0	32.0	41.0
31	37.60986817707081	39.0	37.0	41.0	32.0	41.0
32	37.514909530516476	39.0	37.0	41.0	32.0	41.0
33	37.507010667115715	39.0	37.0	41.0	32.0	41.0
34	37.42820113241376	39.0	36.0	41.0	32.0	41.0
35	37.37125144666491	39.0	36.0	41.0	32.0	41.0
36	37.34908693421897	39.0	36.0	41.0	32.0	41.0
37	37.28406317922682	39.0	36.0	41.0	31.0	41.0
38	37.23580857009645	39.0	36.0	41.0	31.0	41.0
39	37.1734323700581	39.0	36.0	40.0	31.0	41.0
40	37.1083667603369	39.0	36.0	40.0	31.0	41.0
41	37.037969052808016	39.0	36.0	40.0	31.0	41.0
42	36.973966358531506	39.0	36.0	40.0	31.0	41.0
43	36.315530148545356	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	4.0
12	10.0
13	4.0
14	5.0
15	4.0
16	9.0
17	11.0
18	13.0
19	65.0
20	81.0
21	221.0
22	439.0
23	798.0
24	1328.0
25	2396.0
26	3914.0
27	6137.0
28	9191.0
29	13134.0
30	17736.0
31	24064.0
32	31322.0
33	40963.0
34	53864.0
35	73158.0
36	102674.0
37	152301.0
38	258452.0
39	235054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.89057421768158	21.89819956529484	15.079869530049262	23.13135668697432
2	14.415644713699052	23.226551861416073	40.200883232119516	22.156920192765362
3	15.393487988179446	28.53295281219978	33.50938275955042	22.564176440070355
4	12.00773241785036	18.858086187647405	40.34523337904908	28.78894801545315
5	12.3648603268757	38.68691007949479	35.98406794871919	12.964161644910318
6	27.081761753148598	41.46119725939128	18.025079742992496	13.431961244467633
7	24.849444646147468	33.186906672714514	23.950151991068395	18.013496690069626
8	24.586149199455303	35.48258989276429	22.088492577599155	17.84276833018125
9	25.24589653316306	15.330121875130795	20.782041011794274	38.64194057991187
10	14.546367739542887	28.988098656463197	34.336159663137565	22.129373940856347
11	31.697365001464917	24.365973857341565	25.35238275079013	18.58427839040339
12	21.354477433974164	26.764833851326163	30.911664134293332	20.96902458040634
13	28.108662663537622	22.314994797359844	27.247233937761045	22.329108601341492
14	21.582245029264243	22.08021896836853	28.672338793590967	27.665197208776256
15	23.108677264024497	29.35515489656528	24.680857691001133	22.85531014840908
16	22.41213670338527	28.887160623849606	25.29203407169618	23.40866860106895
17	21.31456943650881	29.08845266960169	26.906750583776134	22.690227310113368
18	22.83516147651804	27.485124537286236	27.796893600411927	21.882820385783795
19	24.007385899628467	27.09772495213474	28.00509654328606	20.88979260495073
20	23.52410978398093	26.50708658964748	27.8571449429267	22.111658683444897
21	23.235020143805063	27.036792253565682	27.84653525579566	21.88165234683359
22	21.97344074100391	28.46880800651766	27.889947370111635	21.667803882366798
23	22.189041263896016	27.194866858160164	28.410503395586566	22.205588482357257
24	22.50733187782702	27.855976903976494	27.609326012324757	22.02736520587173
25	22.109614615282037	27.78774396196865	27.744429184231862	22.358212238517446
26	23.102253049798367	27.92596190440964	27.769055338765362	21.20272970702663
27	22.085767153382008	28.20745929140917	27.41007803473553	22.29669552047329
28	21.563945752377688	27.576815594877367	28.806468599706236	22.052770053038703
29	22.87068932792012	27.798061639362132	27.56445384932103	21.766795183396713
30	21.83921359830946	27.673373481427692	28.17154209369035	22.3158708265725
31	23.384431792852187	26.776416904249032	28.194708199536095	21.644443103362686
32	22.160034963299243	27.22095306138142	28.29671693452071	22.322295040798625
33	22.11993229267552	27.508387979711163	28.88015239014837	21.491527337464948
34	22.5416916902789	27.217448944530805	27.99799097300565	22.24286839218465
35	21.72153367407625	27.37104606648283	29.041925784751836	21.865494474689083
36	23.16552182626783	27.242561781960223	27.388079967839996	22.20383642393195
37	22.380015632254615	27.166152567300944	28.259923707589234	22.193908092855203
38	22.33008196713333	26.62924399652314	28.466179918879696	22.574494117463836
39	22.631144006548805	26.750330701027774	28.100291717727817	22.518233574695603
40	21.421639673610983	27.019174332733414	29.675197568921597	21.883988424734003
41	22.226515846881775	26.452286095567	27.96577256529581	23.355425492255417
42	22.209481945524608	26.77992102109965	28.614228855818247	22.396368177557495
43	22.376122169087264	26.040941711936288	29.084851216171888	22.498084902804557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19.0
1	28.5
2	38.0
3	132.0
4	226.0
5	226.0
6	292.0
7	358.0
8	387.0
9	416.0
10	676.0
11	936.0
12	936.0
13	1332.0
14	1728.0
15	4216.0
16	6704.0
17	7758.5
18	8813.0
19	8813.0
20	9142.5
21	9472.0
22	11794.5
23	14117.0
24	17742.0
25	21367.0
26	21367.0
27	25961.5
28	30556.0
29	35443.5
30	40331.0
31	45905.0
32	51479.0
33	51479.0
34	55768.5
35	60058.0
36	63571.5
37	67085.0
38	69741.5
39	72398.0
40	72398.0
41	73056.0
42	73714.0
43	73362.0
44	73010.0
45	73009.0
46	73008.0
47	73008.0
48	71649.5
49	70291.0
50	68951.5
51	67612.0
52	71981.5
53	76351.0
54	76351.0
55	66779.5
56	57208.0
57	54144.0
58	51080.0
59	43107.5
60	35135.0
61	35135.0
62	29607.5
63	24080.0
64	19817.5
65	15555.0
66	12838.5
67	10122.0
68	10122.0
69	8106.0
70	6090.0
71	4955.0
72	3820.0
73	2976.0
74	2132.0
75	2132.0
76	1588.0
77	1044.0
78	824.0
79	604.0
80	412.0
81	220.0
82	220.0
83	158.5
84	97.0
85	77.5
86	58.0
87	37.0
88	16.0
89	16.0
90	14.5
91	13.0
92	7.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1027363.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.32000280704408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58321692983769	44.57863786759263
2	11.901231420358219	13.167523111629956
3	3.250187645669638	5.3940116904559305
4	1.3174288594104304	2.915206728026636
5	0.6437902011802162	1.7807237868218515
6	0.38624585262468736	1.2820272990844106
7	0.2751496375014326	1.065489510325549
8	0.21107291131949338	0.9341243237348272
9	0.14578502460074796	0.7258345173124531
>10	1.0774860369623596	11.735332068135184
>50	0.13182681211890998	5.08216570810128
>100	0.07252035206588632	7.2874672931534965
>500	0.0021173824436469396	0.7950443285036382
>1k	0.0015880368327352045	2.117970759959723
>5k	3.5289707394115655E-4	1.1384410071624227
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	6498	0.6324930915362925	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	5165	0.5027434314842952	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4347	0.42312210971195185	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3843	0.37406447380331975	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	3798	0.369684327740049	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	2983	0.29035501570525707	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1497	0.1457128590381394	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1439	0.14006733744547933	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	1379	0.1342271426944517	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	1314	0.12790026504750512	Illumina Paired End PCR Primer 2 (95% over 23bp)
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	1098	0.10687556394380565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.946731583675877E-4	0.0
5	0.0	0.0	0.0	1.946731583675877E-4	0.0
6	0.0	0.0	0.0	1.946731583675877E-4	0.0
7	0.0	0.0	0.0	1.946731583675877E-4	0.0
8	0.0	0.0	0.0	1.946731583675877E-4	0.0
9	0.0	0.0	0.0	2.9200973755138154E-4	0.0
10	0.0	0.0	0.0	3.893463167351754E-4	0.0
11	0.0	0.0	0.0	3.893463167351754E-4	0.0
12	0.0	0.0	0.0	4.866828959189692E-4	0.0
13	0.0	0.0	0.0	4.866828959189692E-4	0.0
14	0.0	0.0	0.0	4.866828959189692E-4	0.0
15	0.0	0.0	0.0	4.866828959189692E-4	0.0
16	0.0	0.0	0.0	4.866828959189692E-4	0.0
17	0.0	0.0	0.0	4.866828959189692E-4	0.0
18	0.0	0.0	0.0	5.840194751027631E-4	0.0
19	0.0	0.0	0.0	5.840194751027631E-4	0.0
20	0.0	0.0	0.0	5.840194751027631E-4	0.0
21	0.0	0.0	0.0	0.0011680389502055262	0.0
22	0.0	0.0	0.0	0.0016547218461244954	0.0
23	0.0	0.0	0.0	0.0020440681628596706	0.0
24	0.0	0.0	0.0	0.002725424217146228	0.0
25	0.0	0.0	0.0	0.0028227607963300216	0.0
26	0.0	0.0	0.0	0.0030174339546976095	0.0
27	0.0	0.0	0.0	0.00379612658816796	0.0
28	0.0	0.0	0.0	0.010707023710217324	0.0
29	0.0	0.0	0.0	0.025210174008602607	0.0
30	0.0	0.0	0.0	0.043412114315972056	0.0
31	0.0	0.0	0.0	0.09392979891236107	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAGG	20	0.001841845	37.0	36
GGTATCA	725	0.0	29.855173	1
GATTACG	65	2.9904186E-9	28.461536	1
CTTATAC	970	0.0	27.273195	1
GACGTAT	35	8.8687515E-4	26.428572	28
CGTCTTC	230	0.0	24.934784	37
TATACAC	1115	0.0	24.556053	3
TTATACA	1070	0.0	24.551403	2
GTATCAA	2090	0.0	24.253588	1
GCCGTCT	645	0.0	23.806202	36
CTAGTAC	80	2.725028E-8	23.125	3
ATGCGCT	40	0.0019310482	23.125	31
CCGTCTT	665	0.0	23.090225	37
TTACGGG	85	5.1793904E-8	21.764706	3
GCAACGC	70	5.101052E-6	21.142859	3
TAACACG	45	0.0038255355	20.555555	4
CGTACTG	45	0.0038255355	20.555555	14
CGTGCGA	65	6.901221E-5	19.923075	32
TGCCGTC	830	0.0	18.72289	35
ATGCCGT	825	0.0	18.612122	34
>>END_MODULE