FastQCFastQC Report
Fri 10 Feb 2017
ERR1631162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655528
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT120271.8347042384154453No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG108331.6525609890042834No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT92181.4061947010654008TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC57530.8776131606887884No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC32130.4901392465310407RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC27130.41386485398030287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23870.3641339500372219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22460.3426245713379139No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA18280.27885917916549713No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG14730.22470436045447334No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA10060.1534640778120843No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT8270.12615784527892018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8200.12509000378320986No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7650.11669982060262872No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7620.11624217424732429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC4300.033.12790737
GATTACG750.032.0666661
CTTATAC14750.031.2305091
TTATACA16150.028.9814242
TATACAC16550.028.6163143
GGTATCA4850.028.6082481
CGCCGAG358.865313E-426.428572
CCGTCTT15150.026.009937
GCCGTCT15250.025.71803336
TTACGGG951.0913936E-1125.315793
TCGCCGA400.001930307423.1251
GCACGGA400.001930307423.12521
GCTTTAT4200.022.9047641
ATTAGAC652.6799262E-622.769233
CTGCGAC502.7003867E-422.19999918
ATTGAGC4000.021.73756
TGCCGTC18300.021.53278735
GTATCAA11500.020.9130421
TATTGAG4250.020.8941155
CGAACTA909.462747E-820.55555524
Produced by FastQC (version 0.11.5)