FastQCFastQC Report
Fri 10 Feb 2017
ERR1631161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477287
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT101302.1224127202291285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96462.0210062289565816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95011.990626185083608No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG81081.698768246359109No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA54541.1427086847117143No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT50311.0540827636202117TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC46810.9807516232371718No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA36120.7567773687529725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33880.7098454389078269No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT29310.6140959213219719No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA24360.5103847370659582No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT23570.4938328510937864No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC23400.4902710528466101No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT23340.4890139475829009No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG22310.4674336405558919No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC21290.4460628510728346No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC19390.40625451772204146No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG18660.39095973701357883No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG16770.35136092120673723No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG16410.34381828962448174No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC16010.33543758786642003RNA PCR Primer, Index 7 (95% over 22bp)
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT15560.32600929838860054No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG14820.3105050001361864No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG14440.3025433334660278No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG13940.29206745626845065No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG13510.2830582018785343No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13480.2824296492466796No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12880.2698585966095871No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG12830.2688110088898294No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12730.26671583345031397No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG12650.2650396930987016No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12350.25875416678015534No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11960.25058298256604516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.24555456151120814No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG11170.2340310965938733No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG11080.23214543869830942No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10840.2271170176434724No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10490.2197839036051684No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG10490.2197839036051684No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA10010.20972706149549433No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9580.200717807105578No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT9420.1973655264023533No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8970.18793723692453387No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA8950.1875182018366308No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.18688964920477616No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC8480.17767087727090827No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG8230.1724329386721197No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8130.17033776323260427No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7830.16405223691405801No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7700.16132850884268796No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA7640.16007140357897867No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7540.15797622813946327No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG7520.15755719305156016No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA7510.15734767550760864No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA7390.15483346498019013No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7300.15294780708462624No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7290.1527382895406747No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7140.14959552638140156No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG6970.14603372813422533No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6960.14582421059027378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC6840.14331000006285527No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC6810.14268144743100064No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG6770.14184337725519447No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6590.1380720614640667No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6580.13786254392011515No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT6570.1376530263761636No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA6570.1376530263761636No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6500.1361864035685028No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT6480.13576736848059973No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT6460.13534833339269664No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6420.13451026321689047No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6410.13430074567293893No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6360.1332531579531812No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC6230.13052942988181115No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6050.12675811409068338No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG6010.1259200439148772No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC5980.12529149128302258No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5870.12298679829955562No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA5750.12047258777213711No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5620.11774885970076705No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5580.11691078952496088No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA5570.11670127198100932No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG5480.11481561408544544No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG5380.11272043864593002No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA5330.11167285092617231No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA5290.11083478075036614No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA5250.10999671057455997No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA5240.10978719303060841No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG5210.1091586403987538No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA5120.10727298250318991No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT5120.10727298250318991No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5060.10601587723948064No Hit
CCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATG5040.10559684215157757No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT4930.1032921491681106No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4820.10098745618464361No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4800.10056842109674054No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG4780.10014938600883744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTGT251.2325442E-437.037
CCAACGC200.001840857637.03
GATTAGG251.2325442E-437.01
TTATTAC200.001840857637.03
TCAGAGT900.034.9444433
GATTACG900.034.9444431
TTACGGG850.034.823533
CTTATAC12200.033.5122951
CGTCTTC1900.033.10526337
ATTACGG900.032.888892
CTCAGAG900.032.888892
TTATACA12550.032.430282
TATACAC13100.031.3511473
GTTTGTG303.596353E-430.8333321
TAGCAAC850.030.4705871
TACCGCT250.00549316329.635
TTACTTT950.029.2105273
ATTACTT900.028.7777772
AGCAACG851.8189894E-1228.2941172
ACTCAGA1050.028.1904751
Produced by FastQC (version 0.11.5)