FastQCFastQC Report
Fri 10 Feb 2017
ERR1631160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692802
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG145612.101754902555131No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT120051.732818323272739RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT112911.6297585745999579No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC77481.1183570486228387No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC43990.6349577512766996RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC34650.5001428979708489RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28650.4135380671533858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.39766051483685094No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG19410.2801666276944928No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA12290.17739556179110338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10900.1573321093183911No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT10340.1492489917754279No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9660.13943377761611542No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC7320.10565789359730485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC14900.033.7718121
GATTACG551.8535502E-830.2727281
TTATACA17450.029.1547282
TATACAC18450.028.7777793
CGTCTTC5600.028.41071537
GGTATCA4400.027.3295461
CCGTCTT18900.026.72222337
GCCGTCT19500.025.80512836
CGCCGAG601.3361259E-624.6666662
GTATCAA12800.024.281251
TAGGACC551.9012443E-523.5454544
TCGCCGA652.6802918E-622.769231
TGCCGTC23200.021.84913835
ATGCCGT25150.020.1550734
TATGCCG27050.018.7393733
GATACAC1205.162292E-918.53
TTATACT1700.018.54
TATAGGT701.21831006E-418.54
TTACGGG1002.8708382E-718.4999983
CGGCGAT650.001579077617.07692319
Produced by FastQC (version 0.11.5)