##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631160.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692802
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.601194857982513	31.0	30.0	33.0	27.0	34.0
2	30.59637529914752	31.0	30.0	34.0	27.0	34.0
3	29.989369257017156	31.0	30.0	34.0	25.0	34.0
4	34.25458644749871	35.0	35.0	37.0	30.0	37.0
5	34.22752532469595	35.0	35.0	37.0	30.0	37.0
6	34.486313549903144	35.0	35.0	37.0	30.0	37.0
7	34.52622394277153	35.0	35.0	37.0	30.0	37.0
8	34.583953279580605	35.0	35.0	37.0	31.0	37.0
9	36.24473226116553	37.0	35.0	39.0	32.0	39.0
10	35.94516182112638	37.0	35.0	39.0	31.0	39.0
11	36.063917251970985	37.0	35.0	39.0	32.0	39.0
12	35.95265169557825	37.0	35.0	39.0	31.0	39.0
13	36.0790933051579	37.0	35.0	39.0	32.0	39.0
14	36.97677258437476	38.0	36.0	40.0	32.0	41.0
15	36.96897959301503	38.0	36.0	40.0	31.0	41.0
16	36.956993484429894	38.0	36.0	40.0	31.0	41.0
17	36.88573647304713	38.0	36.0	40.0	31.0	41.0
18	36.94986735026747	38.0	36.0	40.0	31.0	41.0
19	36.97593396092967	39.0	36.0	40.0	31.0	41.0
20	36.96800095842679	39.0	36.0	40.0	31.0	41.0
21	36.92873144130646	39.0	36.0	40.0	31.0	41.0
22	36.7924500795321	38.0	36.0	40.0	31.0	41.0
23	36.77679914318954	38.0	36.0	40.0	31.0	41.0
24	36.708781152479354	38.0	35.0	40.0	31.0	41.0
25	36.663264540229385	38.0	35.0	40.0	30.0	41.0
26	36.50284208186466	38.0	35.0	40.0	30.0	41.0
27	36.391176699836315	38.0	35.0	40.0	30.0	41.0
28	36.38543335613927	38.0	35.0	40.0	30.0	41.0
29	36.30273007295013	38.0	35.0	40.0	30.0	41.0
30	36.18756008210138	38.0	35.0	40.0	30.0	41.0
31	36.190032650021216	38.0	35.0	40.0	30.0	41.0
32	36.01125862800627	38.0	35.0	40.0	30.0	41.0
33	35.96729801588332	38.0	35.0	40.0	30.0	41.0
34	35.84583185383414	38.0	34.0	40.0	29.0	41.0
35	35.79693909659614	38.0	34.0	40.0	29.0	41.0
36	35.795006365455066	38.0	34.0	40.0	29.0	41.0
37	35.699761259349714	38.0	34.0	40.0	29.0	41.0
38	35.644669039639034	38.0	34.0	40.0	28.0	41.0
39	35.54381338391055	38.0	34.0	40.0	28.0	41.0
40	35.38675552322309	38.0	34.0	40.0	27.0	41.0
41	35.32872739974769	38.0	34.0	40.0	27.0	41.0
42	35.260524652065094	38.0	34.0	40.0	27.0	41.0
43	34.43495688522839	37.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	12.0
10	13.0
11	26.0
12	12.0
13	9.0
14	13.0
15	13.0
16	23.0
17	26.0
18	52.0
19	93.0
20	210.0
21	409.0
22	785.0
23	1275.0
24	2178.0
25	3445.0
26	5450.0
27	7771.0
28	11212.0
29	15143.0
30	20096.0
31	26064.0
32	33506.0
33	42947.0
34	55605.0
35	73540.0
36	97873.0
37	124089.0
38	125763.0
39	45144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.16992733854694	23.231168501245666	15.905554545165861	23.693349615041527
2	15.000678404508069	22.190322776204457	39.68882884287285	23.120169976414616
3	16.11528257712882	29.147433177155957	33.12071847367646	21.616565772038765
4	12.36154052673058	19.404101027421977	36.45341670491714	31.780941740930306
5	12.403110845522964	38.87084621580192	34.608589467120474	14.117453471554645
6	27.7666634911562	39.70831492980679	17.153241474476115	15.3717801045609
7	24.147736294063815	33.66084393520804	22.33336508843797	19.858054682290177
8	25.39037127490971	34.226084797676684	22.264802930707475	18.11874099670613
9	23.895716236385	14.484080588681902	18.879420094052847	42.74078308088025
10	14.679663164944673	28.030375200995376	33.147421629845184	24.14254000421477
11	31.432068614120627	22.951579239089956	24.34808213602155	21.268270010767868
12	20.54555269759614	26.708641141336198	28.32699674654519	24.418809414522475
13	29.830312268151648	20.104012401811776	25.95864330645697	24.10703202357961
14	22.125802177245447	22.41621704325334	25.984913438471597	29.47306734102962
15	24.429779359759355	28.66547729365677	22.37103818984356	24.53370515674031
16	24.802901839197926	26.715858210570985	23.156255322588564	25.32498462764253
17	20.703750855222705	30.459785046809913	24.545829833054754	24.29063426491263
18	23.719908429825548	24.60327770416367	25.304343809631035	26.372470056379743
19	23.590001183599355	26.891088651591655	25.903649238887876	23.615260925921113
20	22.06229196797931	25.531248466372787	27.087825958931987	25.318633606715917
21	24.79727252519479	24.647157485111187	25.859480775170972	24.696089214523052
22	20.951729354130038	29.343160094803423	27.834503941963217	21.870606609103323
23	23.194072765378852	24.34158677371024	26.33378656528128	26.13055389562963
24	22.406834853248114	27.593020805367193	27.811986685950675	22.188157655434022
25	21.143414713006024	28.088111754873683	26.976683092716247	23.791790439404043
26	22.630419658141864	25.305065516554514	26.30333053311047	25.76118429219315
27	20.918963859804098	28.406961873666646	29.206468803496527	21.467605463032726
28	20.623785728101247	24.342741504787803	28.05549060193244	26.977982165178503
29	21.05247964064769	24.78904506626713	29.820208371222947	24.338266921862235
30	20.87926997901276	25.55636386730985	29.620872918958085	23.9434932347193
31	21.903083420659872	24.21629845179431	27.96715367449863	25.91346445304719
32	22.29294950072315	27.60095958152546	28.935395683037868	21.170695234713527
33	20.869310423468754	25.158992035242395	28.640217551335013	25.33147998995384
34	21.478864091039	28.43626317475989	28.065161474707057	22.019711259494056
35	21.70475835808788	26.428185830872312	31.15175764504144	20.715298165998366
36	23.520428636175993	25.2783623603858	24.845482547683176	26.355726455755036
37	22.234924264075453	26.136471892402156	28.37636150011114	23.25224234341125
38	22.567775497183902	24.530818329046394	28.146136991521388	24.75526918224832
39	24.894269935710348	25.35630670812151	26.555783614943373	23.193639741224768
40	21.469626242418467	23.90004647792587	32.484894674091585	22.14543260556407
41	22.65553505907893	26.04568116142852	26.1829498182742	25.11583396121836
42	22.436424837110746	24.45460607792703	30.431927159563628	22.6770419253986
43	23.568494317279683	23.835525878966862	29.433229118853582	23.162750684899873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	19.0
2	28.0
3	96.0
4	164.0
5	164.0
6	225.5
7	287.0
8	316.5
9	346.0
10	501.0
11	656.0
12	656.0
13	994.0
14	1332.0
15	2966.0
16	4600.0
17	5039.5
18	5479.0
19	5479.0
20	5259.0
21	5039.0
22	5614.5
23	6190.0
24	7882.5
25	9575.0
26	9575.0
27	12071.5
28	14568.0
29	17551.5
30	20535.0
31	23640.5
32	26746.0
33	26746.0
34	30256.0
35	33766.0
36	36480.0
37	39194.0
38	41555.5
39	43917.0
40	43917.0
41	45295.5
42	46674.0
43	47831.0
44	48988.0
45	48890.5
46	48793.0
47	48793.0
48	49258.5
49	49724.0
50	60509.0
51	71294.0
52	56859.5
53	42425.0
54	42425.0
55	50850.5
56	59276.0
57	49885.5
58	40495.0
59	31244.5
60	21994.0
61	21994.0
62	19841.5
63	17689.0
64	14314.5
65	10940.0
66	8909.5
67	6879.0
68	6879.0
69	5718.0
70	4557.0
71	3819.0
72	3081.0
73	3305.0
74	3529.0
75	3529.0
76	2976.0
77	2423.0
78	1763.0
79	1103.0
80	709.0
81	315.0
82	315.0
83	222.5
84	130.0
85	85.5
86	41.0
87	26.5
88	12.0
89	12.0
90	9.5
91	7.0
92	4.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	692802.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.404871960089366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.06583060839856	49.68258944862168
2	9.185323667006836	10.729353053670252
3	2.3196910528005756	4.064437767773475
4	0.9393267750232239	2.1944504009566024
5	0.5100444279515471	1.489453975423357
6	0.34091354673259233	1.1946607227826196
7	0.2252942835189993	0.9210798647587057
8	0.17727870551332203	0.8283152077404768
9	0.1352968682352644	0.711179663929353
>10	0.9641296362844883	10.899166281822033
>50	0.09182451026402962	3.6902045131086614
>100	0.0415808476344373	4.042769821629102
>500	4.950100908823075E-4	0.2454543982941643
>1k	0.00198004036352923	2.714453881724274
>5k	2.4750504544115374E-4	1.1200121778463987
>10k+	7.425151363234612E-4	5.472418819918833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	14561	2.101754902555131	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	12005	1.732818323272739	RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	11291	1.6297585745999579	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	7748	1.1183570486228387	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	4399	0.6349577512766996	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	3465	0.5001428979708489	RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2865	0.4135380671533858	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2755	0.39766051483685094	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1941	0.2801666276944928	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1229	0.17739556179110338	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.1573321093183911	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1034	0.1492489917754279	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	966	0.13943377761611542	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	732	0.10565789359730485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.4434138469577166E-4	0.0	0.0	0.0	0.0
6	1.4434138469577166E-4	0.0	0.0	0.0	1.4434138469577166E-4
7	1.4434138469577166E-4	0.0	0.0	0.0	1.4434138469577166E-4
8	1.4434138469577166E-4	0.0	0.0	1.4434138469577166E-4	2.886827693915433E-4
9	1.4434138469577166E-4	0.0	0.0	2.886827693915433E-4	2.886827693915433E-4
10	4.33024154087315E-4	0.0	0.0	5.773655387830867E-4	2.886827693915433E-4
11	4.33024154087315E-4	0.0	0.0	5.773655387830867E-4	2.886827693915433E-4
12	4.33024154087315E-4	0.0	0.0	5.773655387830867E-4	2.886827693915433E-4
13	4.33024154087315E-4	0.0	0.0	5.773655387830867E-4	4.33024154087315E-4
14	4.33024154087315E-4	0.0	0.0	5.773655387830867E-4	4.33024154087315E-4
15	4.33024154087315E-4	0.0	0.0	7.217069234788583E-4	4.33024154087315E-4
16	4.33024154087315E-4	0.0	0.0	7.217069234788583E-4	5.773655387830867E-4
17	4.33024154087315E-4	0.0	0.0	7.217069234788583E-4	5.773655387830867E-4
18	4.33024154087315E-4	0.0	0.0	7.217069234788583E-4	5.773655387830867E-4
19	4.33024154087315E-4	0.0	0.0	8.6604830817463E-4	5.773655387830867E-4
20	4.33024154087315E-4	0.0	0.0	0.0010103896928704017	5.773655387830867E-4
21	5.773655387830867E-4	0.0	0.0	0.001299072462261945	0.0010103896928704017
22	5.773655387830867E-4	0.0	0.0	0.0018764380010450317	0.0010103896928704017
23	5.773655387830867E-4	0.0	0.0	0.00259814492452389	0.0010103896928704017
24	5.773655387830867E-4	0.0	0.0	0.0031755104633069764	0.0010103896928704017
25	5.773655387830867E-4	0.0	0.0	0.00346419323269852	0.0010103896928704017
26	5.773655387830867E-4	0.0	0.0	0.0036085346173942916	0.0010103896928704017
27	5.773655387830867E-4	0.0	0.0	0.004618924310264693	0.0010103896928704017
28	5.773655387830867E-4	0.0	0.0	0.010681262467487103	0.0010103896928704017
29	5.773655387830867E-4	0.0	0.0	0.02511540093706427	0.0010103896928704017
30	5.773655387830867E-4	0.0	0.0	0.037961784174987946	0.0010103896928704017
31	5.773655387830867E-4	0.0	0.0	0.07260371650197314	0.0010103896928704017
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1490	0.0	33.771812	1
GATTACG	55	1.8535502E-8	30.272728	1
TTATACA	1745	0.0	29.154728	2
TATACAC	1845	0.0	28.777779	3
CGTCTTC	560	0.0	28.410715	37
GGTATCA	440	0.0	27.329546	1
CCGTCTT	1890	0.0	26.722223	37
GCCGTCT	1950	0.0	25.805128	36
CGCCGAG	60	1.3361259E-6	24.666666	2
GTATCAA	1280	0.0	24.28125	1
TAGGACC	55	1.9012443E-5	23.545454	4
TCGCCGA	65	2.6802918E-6	22.76923	1
TGCCGTC	2320	0.0	21.849138	35
ATGCCGT	2515	0.0	20.15507	34
TATGCCG	2705	0.0	18.73937	33
GATACAC	120	5.162292E-9	18.5	3
TTATACT	170	0.0	18.5	4
TATAGGT	70	1.21831006E-4	18.5	4
TTACGGG	100	2.8708382E-7	18.499998	3
CGGCGAT	65	0.0015790776	17.076923	19
>>END_MODULE