FastQCFastQC Report
Fri 10 Feb 2017
ERR1631159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661788
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG107931.6308848150767317No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT83081.2553869214914746RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT79911.207486385368124No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC62210.9400291331967338No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC31500.4759832453897623No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC29970.4528640591851167RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG15270.2307385446698943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13890.2098859453480571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.19598421246683229No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA8920.13478636663100568No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT8460.1278355001903933No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT7040.1063784776998072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC11650.030.8068681
TATACAC13600.027.4779423
CGTCTTC4450.027.0224737
TTATACA13550.026.3505542
GGTATCA2850.024.0175461
CCGTCTT12200.023.95901737
GCCGTCT12500.023.53236
GTATCAA7950.023.270441
TCGCCGA603.7232112E-521.5833341
GTTAACG705.0965264E-621.14285713
GGTCGGT500.007031804418.511
TCGAGCA500.007031804418.59
TGCCGTC15900.018.535
TCTATAC801.6149812E-518.53
CCAATAC852.720289E-517.4117643
TACACAT25050.017.059882
ATGCCGT17250.016.94492734
GCAGACG1005.87021E-616.65000232
ATACACA25850.016.6034831
TAACGCA904.4413722E-516.44444515
Produced by FastQC (version 0.11.5)