##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661788
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.773407798267723	31.0	30.0	33.0	27.0	34.0
2	30.774031865189457	31.0	31.0	34.0	27.0	34.0
3	30.180896299116938	31.0	30.0	34.0	26.0	34.0
4	34.426453788826635	35.0	35.0	37.0	30.0	37.0
5	34.45661299388928	35.0	35.0	37.0	30.0	37.0
6	34.69516370801526	35.0	35.0	37.0	32.0	37.0
7	34.74017359033407	35.0	35.0	37.0	32.0	37.0
8	34.78210695872394	35.0	35.0	37.0	32.0	37.0
9	36.456289627494	38.0	35.0	39.0	32.0	39.0
10	36.18177271271163	37.0	35.0	39.0	32.0	39.0
11	36.293964230236874	38.0	35.0	39.0	32.0	39.0
12	36.19856509939739	38.0	35.0	39.0	32.0	39.0
13	36.31868815995455	38.0	35.0	39.0	32.0	39.0
14	37.27231077021644	39.0	36.0	40.0	32.0	41.0
15	37.28442341051817	39.0	36.0	40.0	32.0	41.0
16	37.28460322641087	39.0	36.0	40.0	32.0	41.0
17	37.21025917665476	39.0	36.0	40.0	32.0	41.0
18	37.28303021511421	39.0	36.0	40.0	32.0	41.0
19	37.28442038840233	39.0	36.0	40.0	32.0	41.0
20	37.31760170930872	39.0	36.0	40.0	32.0	41.0
21	37.258218644037065	39.0	36.0	40.0	32.0	41.0
22	37.129183363856704	39.0	36.0	40.0	31.0	41.0
23	37.15169963795052	39.0	36.0	40.0	31.0	41.0
24	37.07245976052754	39.0	36.0	40.0	31.0	41.0
25	37.05033787255133	39.0	36.0	40.0	31.0	41.0
26	36.89018084341209	39.0	36.0	40.0	31.0	41.0
27	36.7932132344497	39.0	36.0	40.0	31.0	41.0
28	36.785771274184484	39.0	36.0	40.0	31.0	41.0
29	36.660578614299446	38.0	36.0	40.0	30.0	41.0
30	36.60281389206211	38.0	35.0	40.0	30.0	41.0
31	36.597312130168575	38.0	35.0	40.0	30.0	41.0
32	36.47350964357166	38.0	35.0	40.0	30.0	41.0
33	36.46106608158504	38.0	35.0	40.0	30.0	41.0
34	36.3475146119301	38.0	35.0	40.0	30.0	41.0
35	36.324422624768054	38.0	35.0	40.0	30.0	41.0
36	36.335908176032206	38.0	35.0	40.0	30.0	41.0
37	36.26897284326703	38.0	35.0	40.0	30.0	41.0
38	36.22228568665494	38.0	35.0	40.0	30.0	41.0
39	36.167941999552724	38.0	35.0	40.0	30.0	41.0
40	36.07126451371134	38.0	35.0	40.0	29.0	41.0
41	36.017306146379205	38.0	35.0	40.0	29.0	41.0
42	35.97950854352149	38.0	35.0	40.0	29.0	41.0
43	35.19464088197429	38.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	7.0
11	5.0
12	9.0
13	8.0
14	5.0
15	3.0
16	14.0
17	17.0
18	25.0
19	73.0
20	129.0
21	264.0
22	556.0
23	897.0
24	1533.0
25	2558.0
26	4077.0
27	6112.0
28	9000.0
29	12456.0
30	16693.0
31	21516.0
32	28111.0
33	36507.0
34	47559.0
35	63976.0
36	87327.0
37	119720.0
38	143797.0
39	58827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.151625596112346	23.58972964151662	15.705180510979346	23.553464251391684
2	14.827104752579375	22.614946176116824	39.52171994656899	23.03622912473481
3	16.04063536963499	28.527262507026418	33.58794659316881	21.844155530169783
4	12.698175246453546	19.413014439669503	37.13515506476394	30.75365524911301
5	12.479374059366444	38.8213445997812	35.48220880402788	13.217072536824482
6	27.77112912292154	39.75714277079669	17.840003143000477	14.631724963281293
7	24.19883708982333	34.03688190175706	22.855657703071074	18.90862330534854
8	24.70368154152085	35.24346165237206	22.52141169075293	17.53144511535416
9	23.943166089442542	15.56782534588116	19.56124922180517	40.92775934287113
10	14.525799802958048	28.41831523085943	34.09158219852883	22.96430276765369
11	31.294765090935467	23.128403657969017	24.96403682145944	20.61279442963608
12	20.140739934843182	26.7105175675594	29.482855536818438	23.66588696077898
13	28.912733382896032	20.886295913494955	26.60867226362521	23.592298439983804
14	21.68791214104819	22.671913059771406	26.443211421180195	29.196963378000206
15	23.77528755432253	29.135916637956566	22.663904452785484	24.42489135493542
16	24.312619751340307	27.892467074047882	23.723307161810126	24.071606012801684
17	20.80983638264822	30.818328528169143	25.231191862046455	23.14064322713618
18	23.185219435831414	26.875222881043477	25.707326213228406	24.232231469896703
19	23.1238704842034	29.330843109878085	26.372645016228763	21.17264138968975
20	23.05677951247227	26.488240947251988	26.777910750874902	23.677068789400835
21	25.3111268261135	26.476152483877012	25.256879846718284	22.955840843291206
22	21.474701868272014	29.89295665681457	26.090681608007397	22.54165986690602
23	24.64535470573658	26.135862239871376	25.755226749351756	23.463556305040285
24	22.305783725301758	29.253023626901665	25.56060248901461	22.880590158781967
25	22.819845630322703	28.252854388414416	26.707344345923467	22.219955635339414
26	24.90570998567517	28.181985771878608	25.356609669561852	21.555694572884367
27	21.452791528404866	29.016089744752094	27.264169190133398	22.26694953670964
28	24.38756822426517	26.102014542421443	27.03418617442444	22.47623105888895
29	21.479990570998567	27.668830501610785	26.309482795094503	24.541696132296142
30	23.434543992940338	26.908617261116852	28.370716906320453	21.286121839622357
31	24.558922192605486	25.67438515053159	25.4613259835476	24.30536667331532
32	22.334644931609517	28.41665306714537	26.73106795529686	22.51763404594825
33	24.333623456454333	26.168652196776005	28.03239103761325	21.465333309156406
34	21.95808929747895	28.332033823520526	26.303740774991386	23.40613610400914
35	21.92227722473058	27.209015575985056	30.200910261292137	20.66779693799223
36	24.314735232430927	26.244356198661805	25.439566749472643	24.00134181943462
37	22.51506524748107	26.401355116744334	28.304683675134633	22.778895960639964
38	22.445254371490567	25.76353756792205	27.7573784958325	24.03382956475488
39	24.08052125454073	26.39062660549904	26.744969688178088	22.78388245178214
40	21.56445870883123	25.2815100908448	31.05496019873434	22.099071001589632
41	22.695938880729177	26.775190846615534	26.283190387253924	24.245679885401366
42	22.493457119198293	25.37187135457276	29.558861750288614	22.575809775940332
43	23.053001867667593	24.98730711345627	28.99584156859901	22.963849450277127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	10.5
2	18.0
3	48.0
4	78.0
5	78.0
6	94.0
7	110.0
8	123.5
9	137.0
10	223.5
11	310.0
12	310.0
13	476.5
14	643.0
15	1505.0
16	2367.0
17	2804.0
18	3241.0
19	3241.0
20	3686.0
21	4131.0
22	5436.0
23	6741.0
24	9017.0
25	11293.0
26	11293.0
27	14291.5
28	17290.0
29	20417.0
30	23544.0
31	26553.5
32	29563.0
33	29563.0
34	32680.5
35	35798.0
36	38577.5
37	41357.0
38	44108.5
39	46860.0
40	46860.0
41	47957.5
42	49055.0
43	48133.0
44	47211.0
45	46909.0
46	46607.0
47	46607.0
48	45076.5
49	43546.0
50	44010.0
51	44474.0
52	51702.5
53	58931.0
54	58931.0
55	46558.0
56	34185.0
57	38240.0
58	42295.0
59	35261.0
60	28227.0
61	28227.0
62	21705.0
63	15183.0
64	12747.5
65	10312.0
66	8800.5
67	7289.0
68	7289.0
69	5892.0
70	4495.0
71	3684.5
72	2874.0
73	2290.5
74	1707.0
75	1707.0
76	1335.5
77	964.0
78	749.5
79	535.0
80	389.5
81	244.0
82	244.0
83	170.0
84	96.0
85	68.5
86	41.0
87	32.5
88	24.0
89	24.0
90	16.0
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	661788.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.70568446129363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06822877629884	46.78097629601065
2	11.393814601524243	13.149757404120757
3	3.2345112814346217	5.599490621788824
4	1.3134047383834804	3.031636776125001
5	0.7227224622199275	2.0852597178976175
6	0.43903429256419585	1.5200864612638056
7	0.26675993413311183	1.0775495210200576
8	0.21074917731022663	0.9729140421072927
9	0.15930269676152267	0.8273404037838213
>10	1.0648026527326544	11.692856448787738
>50	0.09395521266349122	3.7077813463300204
>100	0.0295736132722389	2.589902071106676
>500	7.851401753659087E-4	0.36879939691085595
>1k	0.001308566958943181	1.5646035020460556
>5k	7.851401753659087E-4	3.4010493113974105
>10k+	2.6171339178863626E-4	1.629996679303386
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	10793	1.6308848150767317	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	8308	1.2553869214914746	RNA PCR Primer, Index 47 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	7991	1.207486385368124	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	6221	0.9400291331967338	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	3150	0.4759832453897623	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	2997	0.4528640591851167	RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	1527	0.2307385446698943	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1389	0.2098859453480571	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1297	0.19598421246683229	No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	892	0.13478636663100568	No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT	846	0.1278355001903933	No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	704	0.1063784776998072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.5110579218722612E-4	0.0
5	0.0	0.0	0.0	1.5110579218722612E-4	0.0
6	0.0	0.0	0.0	1.5110579218722612E-4	0.0
7	0.0	0.0	0.0	1.5110579218722612E-4	0.0
8	0.0	0.0	0.0	1.5110579218722612E-4	0.0
9	0.0	0.0	0.0	1.5110579218722612E-4	0.0
10	0.0	0.0	0.0	1.5110579218722612E-4	0.0
11	0.0	0.0	0.0	1.5110579218722612E-4	0.0
12	0.0	0.0	0.0	1.5110579218722612E-4	0.0
13	0.0	0.0	0.0	1.5110579218722612E-4	0.0
14	0.0	0.0	0.0	1.5110579218722612E-4	0.0
15	0.0	0.0	0.0	1.5110579218722612E-4	0.0
16	0.0	0.0	0.0	1.5110579218722612E-4	0.0
17	0.0	0.0	0.0	1.5110579218722612E-4	0.0
18	0.0	0.0	0.0	1.5110579218722612E-4	0.0
19	0.0	0.0	0.0	1.5110579218722612E-4	0.0
20	0.0	0.0	0.0	1.5110579218722612E-4	0.0
21	0.0	0.0	0.0	1.5110579218722612E-4	0.0
22	0.0	0.0	0.0	6.044231687489045E-4	0.0
23	0.0	0.0	0.0	9.066347531233568E-4	0.0
24	0.0	0.0	0.0	9.066347531233568E-4	0.0
25	0.0	0.0	0.0	9.066347531233568E-4	0.0
26	0.0	0.0	0.0	9.066347531233568E-4	0.0
27	0.0	0.0	0.0	9.066347531233568E-4	0.0
28	0.0	0.0	0.0	0.003928750596867879	0.0
29	0.0	0.0	0.0	0.008461924362484663	0.0
30	0.0	0.0	0.0	0.01903932981559049	0.0
31	0.0	0.0	0.0	0.05349145043427805	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1165	0.0	30.806868	1
TATACAC	1360	0.0	27.477942	3
CGTCTTC	445	0.0	27.02247	37
TTATACA	1355	0.0	26.350554	2
GGTATCA	285	0.0	24.017546	1
CCGTCTT	1220	0.0	23.959017	37
GCCGTCT	1250	0.0	23.532	36
GTATCAA	795	0.0	23.27044	1
TCGCCGA	60	3.7232112E-5	21.583334	1
GTTAACG	70	5.0965264E-6	21.142857	13
GGTCGGT	50	0.0070318044	18.5	11
TCGAGCA	50	0.0070318044	18.5	9
TGCCGTC	1590	0.0	18.5	35
TCTATAC	80	1.6149812E-5	18.5	3
CCAATAC	85	2.720289E-5	17.411764	3
TACACAT	2505	0.0	17.05988	2
ATGCCGT	1725	0.0	16.944927	34
GCAGACG	100	5.87021E-6	16.650002	32
ATACACA	2585	0.0	16.603483	1
TAACGCA	90	4.4413722E-5	16.444445	15
>>END_MODULE