FastQCFastQC Report
Fri 10 Feb 2017
ERR1631158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208660
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG139186.670181155947475No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT104254.996166011693664No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT101654.871561391737755TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC80093.838301543180293No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC38081.8249784338157766No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC33821.6208185565034026RNA PCR Primer, Index 1 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30791.4756062494009392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28981.3888622639700947No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG18750.8985910092974216No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15540.7447522285057031No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA12520.6000191699415317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11700.5607207898015911No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT11050.5295696348126138No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9390.45001437745614875No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9100.43611616984568197No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT8990.4308444359244704No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8950.42892744177130254No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6970.3340362311894949No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6730.32253426627048787No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6380.3057605674302693No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6380.3057605674302693No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5840.2798811463625036No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5360.2568772165244896No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4690.2247675644589284No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4510.21614109076967314No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4510.21614109076967314No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4450.2132655995399214No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3940.18882392408703152No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3900.1869069299338637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.17971820185948434No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3690.17684271062973256No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3610.17300872232339692No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3540.16965398255535322No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3480.16677849132560146No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT3460.16581999424901755No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3380.1619860059426819No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3280.1571935205597623No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3190.15288028371513465No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3170.15192178663855077No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3100.14856704687050706No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3100.14856704687050706No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3020.14473305856417137No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2880.138023579028084No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2880.138023579028084No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA2800.1341895907217483No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2640.12652161410907697No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.12556311703249304No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2610.12508386849420108No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2590.12412537141761719No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2580.12364612287932522No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2520.12077063164957345No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2510.12029138311128151No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2450.11741589188152975No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2440.1169366433432378No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2440.1169366433432378No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2390.11454040065177801No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2350.11262340649861019No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2280.10926866673056648No Hit
CTGATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT2240.10735167257739864No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2220.10639317550081473No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2210.10591392696252278No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2210.10591392696252278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2200.10543467842423081No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2200.10543467842423081No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2200.10543467842423081No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2150.10303843573277102No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2100.10064219304131122No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2090.10016294450301926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA251.2301655E-437.02
GTATACA251.2301655E-437.02
TAGGACC308.279396E-637.04
GATTACG200.001838485637.01
CGAAGCC200.001838485637.034
CTTATAC12100.036.0826451
CGTCTTC4200.034.79762337
TTATACA13400.032.582092
TTAGGAC352.379019E-531.7142853
CTTAGGA352.379019E-531.7142852
CGTTATT303.5894781E-430.83333210
TTGTTAC303.5894781E-430.83333215
GTTGGTT303.5894781E-430.83333237
TATACAC14950.029.9464913
CGTGCCA250.005486166629.610
CCGTGCC250.005486166629.69
TTACGGG250.005486166629.63
TAATGTG250.005486166629.66
GGGGGTG250.005486166629.618
GGTATCA3150.029.3650781
Produced by FastQC (version 0.11.5)