##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631158.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208660
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.796065369500624	31.0	30.0	33.0	27.0	34.0
2	30.815427010447618	31.0	31.0	34.0	27.0	34.0
3	30.204950637400557	31.0	30.0	34.0	26.0	34.0
4	34.39005080034506	35.0	35.0	37.0	30.0	37.0
5	34.58769289753666	35.0	35.0	37.0	32.0	37.0
6	34.84601744464679	35.0	35.0	37.0	32.0	37.0
7	34.84110035464392	35.0	35.0	37.0	32.0	37.0
8	34.89992811271926	35.0	35.0	37.0	32.0	37.0
9	36.61787117799291	38.0	35.0	39.0	32.0	39.0
10	36.412192082814144	38.0	35.0	39.0	32.0	39.0
11	36.46462666538867	38.0	35.0	39.0	32.0	39.0
12	36.30349851432953	38.0	35.0	39.0	32.0	39.0
13	36.43724719639605	38.0	35.0	39.0	32.0	39.0
14	37.11645260231956	39.0	36.0	40.0	31.0	41.0
15	37.14587846257069	39.0	36.0	40.0	32.0	41.0
16	37.18893894373622	39.0	36.0	40.0	32.0	41.0
17	37.143362407744654	39.0	36.0	40.0	31.0	41.0
18	37.33422313811943	39.0	36.0	40.0	32.0	41.0
19	37.25491229751749	39.0	36.0	40.0	32.0	41.0
20	37.233873286686475	39.0	36.0	40.0	32.0	41.0
21	37.219457490654655	39.0	36.0	40.0	31.0	41.0
22	36.93242116361545	39.0	36.0	40.0	31.0	41.0
23	37.06550848269913	39.0	36.0	40.0	31.0	41.0
24	36.81766510112144	38.0	35.0	40.0	31.0	41.0
25	36.81653886705646	38.0	35.0	40.0	31.0	41.0
26	36.63850282756638	38.0	35.0	40.0	30.0	41.0
27	36.33001054346784	38.0	35.0	40.0	30.0	41.0
28	36.44463720885651	38.0	35.0	40.0	30.0	41.0
29	36.29479536087415	38.0	35.0	40.0	30.0	41.0
30	36.12418288124221	38.0	35.0	40.0	30.0	41.0
31	36.02441292054059	38.0	35.0	40.0	30.0	41.0
32	35.72936835042653	38.0	34.0	40.0	29.0	41.0
33	35.703373909709576	38.0	34.0	40.0	29.0	41.0
34	35.55495063740056	38.0	34.0	40.0	29.0	41.0
35	35.52142240966165	38.0	34.0	40.0	28.0	41.0
36	35.446362503594365	38.0	34.0	40.0	28.0	41.0
37	35.31247483945174	38.0	34.0	40.0	27.0	41.0
38	35.16587271158823	38.0	34.0	40.0	26.0	41.0
39	34.97403910668073	38.0	33.0	40.0	26.0	41.0
40	34.5687146554203	37.0	33.0	40.0	24.0	41.0
41	34.49023291478961	37.0	33.0	40.0	24.0	41.0
42	34.29789130643152	37.0	33.0	40.0	23.0	41.0
43	33.236839835138504	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	17.0
11	10.0
12	20.0
13	8.0
14	15.0
15	8.0
16	7.0
17	11.0
18	19.0
19	27.0
20	79.0
21	156.0
22	266.0
23	442.0
24	773.0
25	1269.0
26	1889.0
27	2576.0
28	3746.0
29	5048.0
30	6383.0
31	7887.0
32	9775.0
33	12514.0
34	15933.0
35	20002.0
36	26991.0
37	35152.0
38	39594.0
39	18032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.21508674398543	25.293300105434678	17.82996261861401	24.66165053196588
2	12.515096328956195	21.86427681395572	41.896865714559574	23.723761142528517
3	14.159398063835905	32.918623598198025	35.18978242116362	17.732195916802453
4	9.2073229176651	20.400172529473785	28.25793156330873	42.13457298955238
5	12.24911339020416	39.22026262819898	29.67363174542318	18.85699223617368
6	24.683695964727306	36.41617943065273	16.2038723281894	22.696252276430556
7	20.45959934822199	35.20032588900604	16.00258794210678	28.337486820665198
8	27.137448480782133	27.894181922745137	25.952746094124414	19.015623502348316
9	19.97124508770248	11.511549889772835	14.646314578740535	53.87089044378415
10	15.719352055976229	25.803699798715613	28.425189303172626	30.051758842135534
11	30.24010351768427	19.37314291191412	21.611712834275856	28.775040736125757
12	18.81338061918911	28.30202242883159	20.796990319179525	32.08760663279977
13	36.69893606824499	15.833413208089716	23.007284577782038	24.460366145883256
14	22.587942106776573	25.84060193616409	22.37132176746861	29.20013418959072
15	28.902520847311415	24.841368733825362	18.301063931755007	27.955046487108216
16	28.235406882009013	21.609316591584395	17.71398447234736	32.44129205405923
17	18.488450110227163	33.965302405827664	18.45106872424039	29.095178759704783
18	26.145883255056074	16.139653024058276	23.25649381769386	34.457969903191795
19	24.699511166490943	27.356944311319854	26.46650052717339	21.477043995015816
20	21.778491325601458	25.084826991277676	22.117799290712163	31.018882392408702
21	36.4482890827183	18.112240007667975	23.674877791622738	21.76459311799099
22	22.64065944598869	35.89667401514426	22.043036518738617	19.41963002012844
23	27.008530623981596	19.223138119428736	23.324547110131313	30.443784146458352
24	24.67938272788268	30.36997987156139	27.806000191699415	17.144637208856516
25	20.729895523818655	28.303460174446464	26.91651490462954	24.050129397105337
26	32.836192849611805	21.706124796319372	28.832550560720787	16.62513179334803
27	21.970669989456532	35.148567046870504	26.203393079651104	16.677369884021854
28	24.256685517109172	19.703345154797276	28.654749353014473	27.385219975079078
29	25.646985526694145	24.061631362024347	28.676794785775904	21.614588325505608
30	18.209048212402955	23.625515192178664	33.63222467171475	24.533211923703632
31	31.470334515479724	23.943736221604524	21.047158056167927	23.538771206747818
32	25.881338061918914	21.499089427777246	33.72184414837535	18.897728361928497
33	23.55410715997316	19.534170420780217	32.266845586120965	24.644876833125657
34	16.937122591776095	34.83561775136586	25.605290903862745	22.621968752995304
35	18.614013227259658	19.295983897249116	45.06230230997795	17.027700565513275
36	28.38972491133902	20.031151154988976	22.6876258027413	28.8914981309307
37	21.841752132655994	23.67344004600786	30.85881338061919	23.625994440716955
38	20.185948432857277	17.944982267804082	30.534362120195535	31.334707179143102
39	29.523626952937793	20.1131026550369	25.271254672673248	25.092015719352055
40	17.94258602511262	18.59771877695773	41.98265120291383	21.477043995015816
41	24.867248154893126	22.58890060385316	23.344675548739577	29.19917569251414
42	22.617655516150677	17.901370650819516	36.9308923607783	22.55008147225151
43	25.597622927250075	18.25505607207898	31.555161506757408	24.59215949391354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	3.5
2	6.0
3	43.0
4	80.0
5	80.0
6	122.0
7	164.0
8	211.0
9	258.0
10	364.5
11	471.0
12	471.0
13	774.0
14	1077.0
15	2796.5
16	4516.0
17	4640.5
18	4765.0
19	4765.0
20	3840.0
21	2915.0
22	2246.0
23	1577.0
24	1475.0
25	1373.0
26	1373.0
27	1382.5
28	1392.0
29	1444.0
30	1496.0
31	1624.0
32	1752.0
33	1752.0
34	2147.0
35	2542.0
36	2539.0
37	2536.0
38	3012.0
39	3488.0
40	3488.0
41	4318.0
42	5148.0
43	6557.5
44	7967.0
45	9933.5
46	11900.0
47	11900.0
48	16080.0
49	20260.0
50	31039.0
51	41818.0
52	30188.0
53	18558.0
54	18558.0
55	26666.5
56	34775.0
57	28174.5
58	21574.0
59	14017.0
60	6460.0
61	6460.0
62	5462.0
63	4464.0
64	3461.5
65	2459.0
66	2007.0
67	1555.0
68	1555.0
69	1202.0
70	849.0
71	593.5
72	338.0
73	206.0
74	74.0
75	74.0
76	51.5
77	29.0
78	20.5
79	12.0
80	10.0
81	8.0
82	8.0
83	4.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	208660.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.00948912105818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.89711486118672	16.04428256493818
2	10.815459989112684	4.760854979392313
3	4.413718018508438	2.914310361353398
4	2.5933587370713123	2.2831400364228887
5	1.694066412629287	1.8642768139557175
6	1.2433315187806204	1.641905492188249
7	0.8121937942297224	1.2513179334803028
8	0.6379967338051171	1.12335857375635
9	0.5748502994011976	1.1386945269816926
>10	3.5710397387044095	15.311511549889772
>50	0.42678279804028313	6.5829579219783385
>100	0.2700054436581383	11.51250838684942
>500	0.021774632553075667	3.550752420205119
>1k	0.019597169297768103	9.643918336049074
>5k	0.0021774632553075665	3.838301543180293
>10k+	0.006532389765922701	16.537908559378895
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	13918	6.670181155947475	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	10425	4.996166011693664	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	10165	4.871561391737755	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	8009	3.838301543180293	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3808	1.8249784338157766	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3382	1.6208185565034026	RNA PCR Primer, Index 1 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3079	1.4756062494009392	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2898	1.3888622639700947	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1875	0.8985910092974216	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1554	0.7447522285057031	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	1252	0.6000191699415317	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1170	0.5607207898015911	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	1105	0.5295696348126138	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	939	0.45001437745614875	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	910	0.43611616984568197	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	899	0.4308444359244704	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	895	0.42892744177130254	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	697	0.3340362311894949	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	673	0.32253426627048787	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	638	0.3057605674302693	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	638	0.3057605674302693	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	584	0.2798811463625036	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	536	0.2568772165244896	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	469	0.2247675644589284	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	451	0.21614109076967314	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	451	0.21614109076967314	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	445	0.2132655995399214	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	394	0.18882392408703152	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	390	0.1869069299338637	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	375	0.17971820185948434	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	369	0.17684271062973256	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	361	0.17300872232339692	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	354	0.16965398255535322	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	348	0.16677849132560146	No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT	346	0.16581999424901755	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	338	0.1619860059426819	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	328	0.1571935205597623	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	319	0.15288028371513465	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	317	0.15192178663855077	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	310	0.14856704687050706	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	310	0.14856704687050706	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	302	0.14473305856417137	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	288	0.138023579028084	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	288	0.138023579028084	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	280	0.1341895907217483	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	264	0.12652161410907697	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	262	0.12556311703249304	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	261	0.12508386849420108	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	259	0.12412537141761719	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	258	0.12364612287932522	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	252	0.12077063164957345	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	251	0.12029138311128151	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	245	0.11741589188152975	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	244	0.1169366433432378	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	244	0.1169366433432378	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	239	0.11454040065177801	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	235	0.11262340649861019	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	228	0.10926866673056648	No Hit
CTGATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	224	0.10735167257739864	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	222	0.10639317550081473	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	221	0.10591392696252278	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	221	0.10591392696252278	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	220	0.10543467842423081	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	220	0.10543467842423081	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	220	0.10543467842423081	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	215	0.10303843573277102	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	210	0.10064219304131122	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	209	0.10016294450301926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	4.792485382919582E-4	0.0
12	0.0	0.0	0.0	4.792485382919582E-4	0.0
13	0.0	0.0	0.0	4.792485382919582E-4	0.0
14	0.0	0.0	0.0	4.792485382919582E-4	0.0
15	0.0	0.0	0.0	4.792485382919582E-4	0.0
16	0.0	0.0	0.0	4.792485382919582E-4	0.0
17	0.0	0.0	0.0	4.792485382919582E-4	0.0
18	0.0	0.0	0.0	9.584970765839164E-4	0.0
19	0.0	0.0	0.0	9.584970765839164E-4	0.0
20	0.0	0.0	0.0	9.584970765839164E-4	0.0
21	0.0	0.0	0.0	0.002396242691459791	0.0
22	0.0	0.0	0.0	0.0033547397680437074	0.0
23	0.0	0.0	0.0	0.0038339883063356656	0.0
24	0.0	0.0	0.0	0.00527173392121154	0.0
25	0.0	0.0	0.0	0.00527173392121154	0.0
26	0.0	0.0	0.0	0.005750982459503499	0.0
27	0.0	0.0	0.0	0.008626473689255247	0.0
28	0.0	0.0	0.0	0.014856704687050705	0.0
29	0.0	0.0	0.0	0.041694622831400366	0.0
30	0.0	0.0	0.0	0.06517780120770632	0.0
31	0.0	0.0	0.0	0.11597814626665388	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACA	25	1.2301655E-4	37.0	2
GTATACA	25	1.2301655E-4	37.0	2
TAGGACC	30	8.279396E-6	37.0	4
GATTACG	20	0.0018384856	37.0	1
CGAAGCC	20	0.0018384856	37.0	34
CTTATAC	1210	0.0	36.082645	1
CGTCTTC	420	0.0	34.797623	37
TTATACA	1340	0.0	32.58209	2
TTAGGAC	35	2.379019E-5	31.714285	3
CTTAGGA	35	2.379019E-5	31.714285	2
CGTTATT	30	3.5894781E-4	30.833332	10
TTGTTAC	30	3.5894781E-4	30.833332	15
GTTGGTT	30	3.5894781E-4	30.833332	37
TATACAC	1495	0.0	29.946491	3
CGTGCCA	25	0.0054861666	29.6	10
CCGTGCC	25	0.0054861666	29.6	9
TTACGGG	25	0.0054861666	29.6	3
TAATGTG	25	0.0054861666	29.6	6
GGGGGTG	25	0.0054861666	29.6	18
GGTATCA	315	0.0	29.365078	1
>>END_MODULE