FastQCFastQC Report
Fri 10 Feb 2017
ERR1631157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338249
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT96202.844058666840109No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG87022.57266096869466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68292.0189268852236073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65871.9473819582615175No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT58461.728312574464374Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC48901.4456805489447122No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA38651.1426493500350334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24010.7098321059337944No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23950.708058264769445No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC23920.7071713441872703No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT20610.6093144399539984No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC18240.5392477139621994Illumina PCR Primer Index 11 (95% over 23bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17390.5141182974672505No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16190.4786414741802637No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC15650.4626769037011196No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15530.45912922137242085No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14920.44109516953486927No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14830.43843440778834525No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13160.389062495380622No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG12440.3677764014084299No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11480.3393949427788404No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10020.29623147444633985No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9950.2941619930879323No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9540.28204074513154515No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9500.2808581843553122No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9290.27464974028008954No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG9210.2722846187276237No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9090.26873693639892504No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9030.26696309523457573No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8430.24922468359108232No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA8410.24863340320296587No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8370.247450842426733No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8020.23710343563469516No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7730.2285298700070067No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7700.22764294942483201No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.22320834651395868No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7440.2199563043793182No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7280.21522606127438662No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA7190.2125652995278626No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT7190.2125652995278626No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6990.20665249564669813No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6910.20428737409423237No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6840.20221789273582477No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6820.20162661234770837No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6430.19009664477943763No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5740.1696974713894202No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT5680.16792363022507087No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5640.166741069448838No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.16408030770231397No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5500.16260210673202286No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5480.1620108263439064No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5470.1617151861498482No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5270.15580238226868373No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5230.15461982149245082No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5220.1543241812983926No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5210.1540285411043344No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA5100.15077649896969392No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT5070.14988957838751926No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4890.14456805489447125No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4870.1439767745063548No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4860.14368113431229657No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4810.14220293334200543No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4800.14190729314794723No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4540.13422064810243342No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4490.13274244713214228No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4490.13274244713214228No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4430.13096860596779294No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4420.13067296577373474No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4350.12860348441532718No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4290.12682964325097784No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4260.12594272266880316No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4260.12594272266880316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC4240.1253514422806867No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4240.1253514422806867No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4220.12476016189257026No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4210.12446452169851203No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4190.1238732413103956No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4160.12298632072822092No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4090.12091683936981336No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4070.12032555898169692No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG4040.11943863839952225No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4010.11855171781734758No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3810.11263891393618311No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3750.11086507277183377No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3750.11086507277183377No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3740.11056943257777554No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3710.10968251199560088No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT3590.1061348296669022No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA3540.10465662869661106No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA3540.10465662869661106No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3510.1037697081144364No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA3490.10317842772631997No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3450.10199586695008706No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG3420.10110894636791239No Hit
GTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCT3400.10051766597979596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG1050.037.0000043
GATTACG1050.037.0000041
TGTATCC251.231784E-437.03
TCTACAC200.001840100337.03
TCAGAGT355.6156387E-737.03
ACAGACA251.231784E-437.017
CGTTTAT200.001840100337.01
CGTCTTC2150.034.41860637
CTTATAC10100.034.069311
ATTACGG1150.033.7826082
TTATACA10650.032.4835662
TAGCAAC401.5956502E-632.3751
ACTCAGA401.5956502E-632.3751
GGTATCA8050.031.94411
TATACAC11000.031.453
GACAGAC303.5941566E-430.83333216
TTGTATC303.5941566E-430.8333322
CAGACAC303.5941566E-430.83333218
TTACTTT250.005490927529.63
TTATCAA502.7111855E-729.62
Produced by FastQC (version 0.11.5)