FastQCFastQC Report
Fri 10 Feb 2017
ERR1631156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415617
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG79671.9169090773476543No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT73981.7800041865467484RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT64021.5403604761114198No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC44551.0719003313146478No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC22800.5485819877435235RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC22790.5483413816085483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18810.45258013988840684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.4383843779248683No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG11120.2675540220924553No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA7470.17973278282649652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6960.1674618699427598No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT6880.16553702086295796No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT6070.14604792392996438No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4800.1154909447881102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC8550.033.9707571
CGTCTTC2550.031.19607737
TTATACA9850.029.8629422
CGGATCG250.005492356629.637
CCGTCTT9800.027.18367237
TATACAC11050.026.7873293
GGTATCA2700.026.7222231
GATTACG358.8598306E-426.428571
GCCGTCT10150.026.06403736
ACGGGCT451.3212272E-424.66666616
GTATCAA8550.024.6666641
GTTCGCC400.001929124423.12524
TACCCTC855.160655E-821.7647065
TGCCGTC12750.021.18431335
TTGTACT705.0889994E-621.1428574
CGGTTCG450.003821760620.55555522
AGACGTC450.003821760620.5555556
TACCGCA555.135504E-420.1818187
ATGCCGT13500.020.00740634
TCGCCTC656.888587E-519.92307726
Produced by FastQC (version 0.11.5)