##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415617
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08390898351126	31.0	31.0	34.0	28.0	34.0
2	31.095833423560634	31.0	31.0	34.0	28.0	34.0
3	30.518162154098604	31.0	30.0	34.0	26.0	34.0
4	34.72637067299942	37.0	35.0	37.0	32.0	37.0
5	34.88096733290505	35.0	35.0	37.0	32.0	37.0
6	35.10936270653029	36.0	35.0	37.0	32.0	37.0
7	35.118529800272846	36.0	35.0	37.0	32.0	37.0
8	35.15432958709581	37.0	35.0	37.0	32.0	37.0
9	36.865282218966016	39.0	37.0	39.0	33.0	39.0
10	36.655933226985425	38.0	35.0	39.0	32.0	39.0
11	36.72623833962518	38.0	35.0	39.0	32.0	39.0
12	36.64244003493601	38.0	35.0	39.0	32.0	39.0
13	36.75117475945402	39.0	37.0	39.0	32.0	39.0
14	37.747563261368036	39.0	37.0	40.0	33.0	41.0
15	37.72461665427545	39.0	37.0	40.0	33.0	41.0
16	37.74958435290183	39.0	37.0	40.0	33.0	41.0
17	37.71890466463114	39.0	37.0	40.0	33.0	41.0
18	37.79766227079258	39.0	37.0	40.0	33.0	41.0
19	37.81799108313664	39.0	37.0	41.0	33.0	41.0
20	37.81244751778681	39.0	37.0	41.0	33.0	41.0
21	37.76377770880402	39.0	37.0	40.0	33.0	41.0
22	37.645149260015835	39.0	37.0	40.0	32.0	41.0
23	37.667352394151344	39.0	37.0	40.0	32.0	41.0
24	37.60285791967124	39.0	37.0	40.0	32.0	41.0
25	37.55717643888484	39.0	37.0	40.0	32.0	41.0
26	37.431173412059664	39.0	36.0	40.0	32.0	41.0
27	37.363543839640826	39.0	36.0	40.0	32.0	41.0
28	37.32652177365218	39.0	36.0	40.0	32.0	41.0
29	37.26400026947887	39.0	36.0	40.0	31.0	41.0
30	37.167437809329265	39.0	36.0	40.0	31.0	41.0
31	37.113392859291125	39.0	36.0	40.0	31.0	41.0
32	37.00531739558295	39.0	36.0	40.0	31.0	41.0
33	36.89038465702799	39.0	36.0	40.0	31.0	41.0
34	36.810041456437055	39.0	36.0	40.0	30.0	41.0
35	36.813809348510766	39.0	36.0	40.0	30.0	41.0
36	36.766535055110836	39.0	35.0	40.0	30.0	41.0
37	36.70934778894992	39.0	35.0	40.0	30.0	41.0
38	36.64921309763557	39.0	35.0	40.0	30.0	41.0
39	36.52780324192706	39.0	35.0	40.0	30.0	41.0
40	36.371965054364956	38.0	35.0	40.0	30.0	41.0
41	36.34812820457296	38.0	35.0	40.0	30.0	41.0
42	36.27458453335643	38.0	35.0	40.0	30.0	41.0
43	35.506733362687285	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	17.0
11	6.0
12	12.0
13	8.0
14	3.0
15	11.0
16	11.0
17	14.0
18	20.0
19	30.0
20	57.0
21	133.0
22	272.0
23	480.0
24	818.0
25	1344.0
26	2094.0
27	3163.0
28	4656.0
29	6531.0
30	8790.0
31	11799.0
32	15002.0
33	19121.0
34	25439.0
35	34079.0
36	46953.0
37	68723.0
38	103407.0
39	62615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.16622996653169	24.378213595690262	16.436045686292907	23.019510751485143
2	13.633465426101434	22.966818007925564	40.91868234456243	22.481034221410578
3	14.646176648212176	29.631126734469476	35.17541390270369	20.547282714614656
4	11.201659219906789	19.697461845882145	38.314361539590536	30.786517394620528
5	11.373211394144128	39.63143952244494	35.61187343154876	13.38347565186217
6	25.97126681536126	41.14653635438397	18.10128074645647	14.780916083798305
7	22.544794847179013	35.23965574074207	23.056804702406303	19.158744709672607
8	24.46362877360647	35.25096422908591	22.992081652097966	17.293325345209652
9	22.948050729397497	15.384356270316179	19.93325585815787	41.73433714212845
10	13.58582541137634	29.013971998258008	34.50821309041738	22.89198949994827
11	29.954260773741208	23.686230351501504	25.525904859522107	20.83360401523518
12	19.32091324464591	28.030855330749223	29.422280609310974	23.22595081529389
13	28.476698498858322	21.15048229499756	27.242389026435397	23.13043017970872
14	21.21857383119555	23.72568975763744	27.198117497599956	27.857618913567055
15	23.189859895047604	29.921297733249602	23.563040010394186	23.325802361308607
16	23.754081281564517	28.523376089043516	23.99107832451512	23.731464304876845
17	19.710213971035834	31.802356496485945	25.595921244799904	22.89150828767832
18	22.272669308522026	26.35238693316202	27.14734960312018	24.22759415519577
19	22.52025302141154	28.566925799474035	28.81835921052315	20.094461968591276
20	21.374486606659495	28.966572589667894	26.67576157856873	22.983179225103882
21	23.764427345368453	28.02267472216007	26.92454832213312	21.288349610338365
22	21.815998864339043	31.4385600204034	26.893991342991264	19.851449772266292
23	23.5755515294129	26.49626940187721	27.271502368767397	22.656676699942498
24	22.519049990736665	28.48752577493221	27.49646910496924	21.49695512936189
25	22.888139801788665	29.37824968661051	26.560992452185545	21.172618059415278
26	21.80805886188486	29.719910398275335	28.470683345483945	20.00134739435586
27	21.601137585806164	31.67676009402888	26.711371286545067	20.010731033619894
28	20.407731156329024	28.856615585984212	28.987024111140787	21.748629146545976
29	22.318865686437274	26.8227719270386	28.639107639966603	22.219254746557528
30	22.842184030008397	28.477660923398222	28.965850771262964	19.714304275330413
31	21.125459256960134	28.648491279230637	26.6974161307165	23.528633333092728
32	25.123611401843526	26.713055529489893	28.133353544248674	20.02997952441791
33	21.240950201748245	28.13960930375803	30.241063286631682	20.378377207862048
34	20.341564469210834	29.49325941912867	27.309758744228464	22.855417367432036
35	21.8980455563656	26.652903995746087	32.03646626581684	19.412584182071473
36	22.12469653551226	28.49835305100609	26.21427901168624	23.162671401795404
37	21.227476258189633	26.879314368757772	29.560388530786756	22.332820842265836
38	21.218092618925596	25.67676490615158	29.368866047346476	23.73627642757635
39	23.77573583371229	26.574707001879133	27.818400113566096	21.83115705084248
40	19.82690794649882	25.70347218713383	33.34103272965254	21.128587136714813
41	21.539061202982555	27.085032614161598	27.308074501283635	24.067831681572216
42	21.380020547763927	25.752555838668773	31.47777882040436	21.389644793162937
43	21.977686187042398	25.27014053804344	30.61785249400289	22.13432078091127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	10.0
2	16.0
3	59.5
4	103.0
5	103.0
6	155.0
7	207.0
8	203.5
9	200.0
10	313.0
11	426.0
12	426.0
13	651.5
14	877.0
15	2067.5
16	3258.0
17	3721.0
18	4184.0
19	4184.0
20	4472.5
21	4761.0
22	5949.0
23	7137.0
24	8652.5
25	10168.0
26	10168.0
27	11822.0
28	13476.0
29	15574.5
30	17673.0
31	19269.5
32	20866.0
33	20866.0
34	22310.5
35	23755.0
36	25101.5
37	26448.0
38	27311.5
39	28175.0
40	28175.0
41	28548.5
42	28922.0
43	28074.0
44	27226.0
45	27098.5
46	26971.0
47	26971.0
48	26949.0
49	26927.0
50	33147.0
51	39367.0
52	31445.0
53	23523.0
54	23523.0
55	27660.0
56	31797.0
57	25995.0
58	20193.0
59	15085.0
60	9977.0
61	9977.0
62	8512.0
63	7047.0
64	5797.0
65	4547.0
66	3801.5
67	3056.0
68	3056.0
69	2425.0
70	1794.0
71	1483.0
72	1172.0
73	940.5
74	709.0
75	709.0
76	536.0
77	363.0
78	282.5
79	202.0
80	122.5
81	43.0
82	43.0
83	30.0
84	17.0
85	17.0
86	17.0
87	12.0
88	7.0
89	7.0
90	6.0
91	5.0
92	2.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	415617.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.938908289783726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.50172019862117	47.82872884809418
2	8.408283122325168	9.407003569085678
3	2.082669785565577	3.4950682239796906
4	0.9819367373868959	2.197138763962201
5	0.5655415888361573	1.5817889535982188
6	0.3854688402387443	1.2937623661610647
7	0.26758477780958617	1.0477880241942816
8	0.21509948189638028	0.9625944152785288
9	0.17054584420308322	0.8586133504272015
>10	1.2507592199907063	13.579995888474262
>50	0.1187569476337181	4.540196981459239
>100	0.04603983114773404	3.9805851497228204
>500	0.0017211151830928612	0.6590181485029075
>1k	0.0025816727746392916	3.3285471057876945
>5k	0.0012908363873196458	5.239170211272018
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	7967	1.9169090773476543	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7398	1.7800041865467484	RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	6402	1.5403604761114198	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	4455	1.0719003313146478	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2280	0.5485819877435235	RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2279	0.5483413816085483	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1881	0.45258013988840684	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1822	0.4383843779248683	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	1112	0.2675540220924553	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	747	0.17973278282649652	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	696	0.1674618699427598	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	688	0.16553702086295796	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	607	0.14604792392996438	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	480	0.1154909447881102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	2.406061349752296E-4	0.0
21	0.0	0.0	0.0	9.624245399009184E-4	0.0
22	0.0	0.0	0.0	0.0014436368098513777	0.0
23	0.0	0.0	0.0	0.002406061349752296	0.0
24	0.0	0.0	0.0	0.0033684858896532144	0.0
25	0.0	0.0	0.0	0.003609092024628444	0.0
26	0.0	0.0	0.0	0.004571516564529363	0.0
27	0.0	0.0	0.0	0.004571516564529363	0.0
28	0.0	0.0	0.0	0.007218184049256888	0.0
29	0.0	0.0	0.0	0.015639398773389924	0.0
30	0.0	0.0	0.0	0.025504250307374337	0.0
31	0.0	0.0	0.0	0.05269274355957528	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	855	0.0	33.970757	1
CGTCTTC	255	0.0	31.196077	37
TTATACA	985	0.0	29.862942	2
CGGATCG	25	0.0054923566	29.6	37
CCGTCTT	980	0.0	27.183672	37
TATACAC	1105	0.0	26.787329	3
GGTATCA	270	0.0	26.722223	1
GATTACG	35	8.8598306E-4	26.42857	1
GCCGTCT	1015	0.0	26.064037	36
ACGGGCT	45	1.3212272E-4	24.666666	16
GTATCAA	855	0.0	24.666664	1
GTTCGCC	40	0.0019291244	23.125	24
TACCCTC	85	5.160655E-8	21.764706	5
TGCCGTC	1275	0.0	21.184313	35
TTGTACT	70	5.0889994E-6	21.142857	4
CGGTTCG	45	0.0038217606	20.555555	22
AGACGTC	45	0.0038217606	20.555555	6
TACCGCA	55	5.135504E-4	20.181818	7
ATGCCGT	1350	0.0	20.007406	34
TCGCCTC	65	6.888587E-5	19.923077	26
>>END_MODULE