FastQCFastQC Report
Fri 10 Feb 2017
ERR1631155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171583
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG122797.156303363386815No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT112436.5525139436890605RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT68343.9829120600525694No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC65873.8389584049701893No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC50172.9239493423008107RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC36452.1243363270254045RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28581.6656661790503722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24131.4063164765740197No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG14740.8590594639329071No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10970.6393407272282219No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA10860.6329298357063345No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT10090.588053595053123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9890.5763974286496913No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT8270.4819824807818956No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7920.46158418957589037No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7880.4592529562952041No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7470.43535781516816935No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5580.3252070426557409No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5030.2931525850463041No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4970.28965573512527465No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4710.2745027188008136No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4470.26051531911669573No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4420.25760127751583783No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4240.24711072775274937No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT3790.22088435334502834No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3550.2068969536609105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.20165167877936627No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3380.19698921221799362No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3330.19407517061713575No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3270.19057832069610625No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3130.18241900421370416No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2870.1672659878892431No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2810.16376913796821363No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2780.16202071300769888No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2680.1561926298059831No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2620.15269577988495364No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2540.14803331332358102No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2520.14686769668323785No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.14628488836306627No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2360.13754276356049258No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2330.13579433859997786No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2330.13579433859997786No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2290.13346310531929154No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2250.1311318720386052No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA2220.1293834470780905No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2220.1293834470780905No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2220.1293834470780905No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2190.12763502211757574No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2180.12705221379740417No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2150.12530378883688942No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT2120.12355536387637468RNA PCR Primer, Index 27 (100% over 23bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2120.12355536387637468No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2110.12297255555620311No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2050.11947570563517365No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2040.11889289731500208No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1980.11539604739397259No Hit
CTGATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT1950.11364762243345787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1860.10840234755191366No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1780.10373988099054102No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1760.10257426435019786No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1730.10082583938968313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA200.001837574537.01
ACAGACA355.5941564E-737.017
GCAACGC251.2292524E-437.03
TAGCAAC251.2292524E-437.01
CAGACAC355.5941564E-737.018
AGCAACG251.2292524E-437.02
TTTACAC308.270801E-637.02
CGTCTTC5450.035.30275337
CTTATAC8600.035.2790681
GACAGAC401.5895839E-632.37516
TTATACA10200.029.7450982
ACTGTGC250.005483479229.68
AGGTGGC250.005483479229.67
CGGGCAC250.005483479229.616
GTCTTCT250.005483479229.637
GGTGGCT250.005483479229.68
GACACAT453.9821825E-628.77777720
AGACAGA453.9821825E-628.77777715
GGTATCA2800.028.4107151
TATACAC11500.026.7043483
Produced by FastQC (version 0.11.5)