##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631155.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171583
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.3603970090277	31.0	30.0	33.0	27.0	34.0
2	30.360006527453187	31.0	30.0	33.0	26.0	34.0
3	29.799374063864136	31.0	30.0	34.0	25.0	34.0
4	33.96887220761963	35.0	33.0	37.0	28.0	37.0
5	33.860271705238866	35.0	33.0	37.0	28.0	37.0
6	34.11908522406066	35.0	35.0	37.0	30.0	37.0
7	34.15769044718883	35.0	35.0	37.0	30.0	37.0
8	34.20728743523542	35.0	35.0	37.0	30.0	37.0
9	35.854134733627454	37.0	35.0	39.0	30.0	39.0
10	35.61743879055617	37.0	35.0	39.0	30.0	39.0
11	35.66446559391082	37.0	35.0	39.0	30.0	39.0
12	35.48566000128218	37.0	35.0	39.0	30.0	39.0
13	35.65757679956639	37.0	35.0	39.0	30.0	39.0
14	36.16762732904775	38.0	35.0	40.0	30.0	41.0
15	36.18159141639906	38.0	35.0	40.0	30.0	41.0
16	36.21861722897956	38.0	35.0	40.0	30.0	41.0
17	36.14323680084857	38.0	35.0	40.0	30.0	41.0
18	36.3862037614449	38.0	35.0	40.0	30.0	41.0
19	36.29910888607846	38.0	35.0	40.0	30.0	41.0
20	36.04800009324933	38.0	34.0	40.0	29.0	41.0
21	35.65007022840258	38.0	34.0	40.0	28.0	41.0
22	35.67957781365287	38.0	34.0	40.0	29.0	41.0
23	35.73703105785538	38.0	34.0	40.0	29.0	41.0
24	35.64031984520611	38.0	34.0	40.0	28.0	41.0
25	35.60670928938182	38.0	34.0	40.0	28.0	41.0
26	35.16084926828415	37.0	33.0	40.0	27.0	41.0
27	35.005938816782546	37.0	33.0	40.0	27.0	41.0
28	34.64409061503762	37.0	33.0	39.0	25.0	40.0
29	34.91185606965725	37.0	33.0	39.0	26.0	40.0
30	34.722000431278154	37.0	33.0	39.0	25.0	40.0
31	34.42962298129768	37.0	33.0	39.0	25.0	40.0
32	34.36126539342476	37.0	33.0	39.0	25.0	40.0
33	34.25070665508821	37.0	33.0	39.0	25.0	40.0
34	34.15816252192816	37.0	33.0	39.0	24.0	40.0
35	34.16529609576706	37.0	33.0	39.0	24.0	40.0
36	34.01060711142712	37.0	33.0	39.0	24.0	40.0
37	33.85859904535997	36.0	32.0	39.0	23.0	40.0
38	33.663055197776	36.0	32.0	39.0	23.0	40.0
39	33.32273010729501	36.0	31.0	39.0	22.0	40.0
40	32.8196208249069	36.0	31.0	39.0	20.0	40.0
41	32.799747061189045	36.0	31.0	39.0	20.0	40.0
42	32.52909670538457	35.0	30.0	39.0	18.0	40.0
43	31.380987626979362	35.0	28.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	13.0
10	20.0
11	24.0
12	11.0
13	11.0
14	11.0
15	14.0
16	10.0
17	23.0
18	28.0
19	70.0
20	129.0
21	284.0
22	436.0
23	745.0
24	1181.0
25	1771.0
26	2570.0
27	3582.0
28	4709.0
29	6062.0
30	7576.0
31	9471.0
32	11428.0
33	13862.0
34	17008.0
35	19727.0
36	22079.0
37	22180.0
38	19799.0
39	6744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.68893771527482	25.355658777384704	19.49901796798051	24.45638553935996
2	12.3631128957997	22.165948841085655	40.61358059947664	24.857357663638005
3	15.045196785229306	32.29632306230803	34.872335837466416	17.786144314996243
4	9.367478130117785	18.975656096466434	25.882517498819812	45.774348274595965
5	13.482687678849302	38.82669028983058	27.25036862626251	20.44025340505761
6	26.018311837419787	35.04251586695651	15.411783218617229	23.527389077006465
7	21.147199897425736	36.18132332457178	14.779436191231065	27.892040586771415
8	29.257560480933424	25.581205597291106	25.46056427501559	19.700669646759877
9	19.12252379314967	11.146209123281443	13.753110739408916	55.97815634415997
10	15.539418240734804	24.54147555410501	27.292913633635035	32.62619257152515
11	29.478444834278456	19.567206541440584	19.721650746286056	31.23269787799491
12	17.932429203359305	28.980143720531753	19.87376371785084	33.2136633582581
13	37.937907601568924	14.970597320247345	21.23054148721027	25.860953590973462
14	23.818210428772083	27.002092281869416	21.597710728918365	27.581986560440136
15	30.127110494629424	25.68203143668079	17.603725310782536	26.58713275790725
16	28.59082776265714	22.0604605351346	16.843160452958628	32.505551249249635
17	17.79255520651813	33.922358275586745	18.14398862358159	30.14109789431354
18	27.46192804648479	15.010811094339182	21.172260655193114	36.35500020398291
19	33.96665170791978	25.127780724197617	20.788189972200044	20.117377595682555
20	26.123217335050676	22.033068544086536	21.456088307116673	30.387625813746116
21	31.79802194856134	23.639288274479405	21.396059050138998	23.166630726820255
22	19.327089513529895	32.74042300227878	24.08338821445015	23.849099269741174
23	26.027053962222364	18.422571000623606	28.71554874317386	26.834826293980175
24	25.836475641526256	23.888730235512842	21.696788143347533	28.578005979613362
25	21.036466316593135	22.910777874264934	26.573728166543304	29.479027642598627
26	28.63278996170949	23.922533118082796	28.466689590460593	18.97798732974712
27	25.087566950105778	27.057459072285717	25.92972497275371	21.925249004854795
28	15.792939860009442	24.459882389280992	34.9813209933385	24.76585675737107
29	21.929328663095994	18.72737975207334	23.52622346036612	35.81706812446455
30	19.938455441389884	17.578081744694988	39.663603037596964	22.819859776318165
31	21.295233210749316	19.900572900578727	25.58819929713317	33.21599459153879
32	20.91232814439659	19.973423940600178	28.4276414330091	30.68660648199414
33	15.219456472960607	18.774587226007238	45.83437753157364	20.171578769458513
34	15.985266605666062	30.230850375619962	26.193154333471263	27.59072868524271
35	18.120676290774725	17.927183928477763	42.605619437823094	21.346520342924418
36	23.44113344562107	19.034519736803762	27.286502742113143	30.23784407546202
37	21.23287272049096	21.531853388738977	31.38189680795883	25.853377082811235
38	20.970026168093575	16.823927778392964	29.161397108105113	33.04464894540835
39	31.4337667484541	18.980318563027808	25.765373026465326	23.82054166205277
40	17.225482710991184	17.463268505621183	42.71984986857672	22.59139891481091
41	24.528653771061233	21.802859257618763	24.162067337673314	29.50641963364669
42	21.054533374518456	16.773223454538037	40.21610532511962	21.956137845823886
43	25.27872807912206	17.208581269706205	31.83182483113129	25.68086582004045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	27.0
4	53.0
5	53.0
6	101.5
7	150.0
8	173.5
9	197.0
10	305.5
11	414.0
12	414.0
13	715.5
14	1017.0
15	2649.0
16	4281.0
17	4210.5
18	4140.0
19	4140.0
20	3432.5
21	2725.0
22	2126.0
23	1527.0
24	1403.0
25	1279.0
26	1279.0
27	1229.5
28	1180.0
29	1117.0
30	1054.0
31	1050.5
32	1047.0
33	1047.0
34	1240.5
35	1434.0
36	1418.0
37	1402.0
38	1531.5
39	1661.0
40	1661.0
41	2198.5
42	2736.0
43	3782.5
44	4829.0
45	6513.0
46	8197.0
47	8197.0
48	10554.5
49	12912.0
50	14017.0
51	15122.0
52	24762.0
53	34402.0
54	34402.0
55	24178.5
56	13955.0
57	21836.0
58	29717.0
59	23398.5
60	17080.0
61	17080.0
62	10714.0
63	4348.0
64	3279.5
65	2211.0
66	1783.0
67	1355.0
68	1355.0
69	1054.5
70	754.0
71	529.5
72	305.0
73	181.0
74	57.0
75	57.0
76	39.0
77	21.0
78	16.5
79	12.0
80	7.5
81	3.0
82	3.0
83	2.0
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	1.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	171583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.713205853726766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70378733449657	17.421889114889005
2	9.30924766499025	4.228857171164976
3	3.861746895206815	2.631379565574678
4	2.2477676280406444	2.042160353881212
5	1.2906702247767627	1.4657629252315205
6	0.9955865749769066	1.3567777693594354
7	0.723596428204865	1.1504636240186965
8	0.4977932874884533	0.9045185129062902
9	0.4054192753771939	0.828753431283985
>10	3.2382223134558146	14.752627008503175
>50	0.4182489992815354	6.595641759381757
>100	0.25916042286769986	11.217894546662547
>500	0.01796161346607821	3.032934498172896
>1k	0.01796161346607821	7.9157026045703835
>5k	0.007697834342604948	10.74581980732357
>10k+	0.005131889561736632	13.708817307075877
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	12279	7.156303363386815	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	11243	6.5525139436890605	RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	6834	3.9829120600525694	No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	6587	3.8389584049701893	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	5017	2.9239493423008107	RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	3645	2.1243363270254045	RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2858	1.6656661790503722	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2413	1.4063164765740197	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	1474	0.8590594639329071	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1097	0.6393407272282219	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	1086	0.6329298357063345	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	1009	0.588053595053123	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	989	0.5763974286496913	No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	827	0.4819824807818956	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	792	0.46158418957589037	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	788	0.4592529562952041	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	747	0.43535781516816935	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	558	0.3252070426557409	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	503	0.2931525850463041	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	497	0.28965573512527465	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	471	0.2745027188008136	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	447	0.26051531911669573	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	442	0.25760127751583783	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	424	0.24711072775274937	No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT	379	0.22088435334502834	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	355	0.2068969536609105	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	346	0.20165167877936627	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	338	0.19698921221799362	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	333	0.19407517061713575	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	327	0.19057832069610625	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	313	0.18241900421370416	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	287	0.1672659878892431	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	281	0.16376913796821363	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	278	0.16202071300769888	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	268	0.1561926298059831	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	262	0.15269577988495364	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	254	0.14803331332358102	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	252	0.14686769668323785	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	251	0.14628488836306627	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	236	0.13754276356049258	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	233	0.13579433859997786	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	233	0.13579433859997786	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	229	0.13346310531929154	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	225	0.1311318720386052	No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	222	0.1293834470780905	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	222	0.1293834470780905	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	222	0.1293834470780905	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	219	0.12763502211757574	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	218	0.12705221379740417	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	215	0.12530378883688942	No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT	212	0.12355536387637468	RNA PCR Primer, Index 27 (100% over 23bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	212	0.12355536387637468	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	211	0.12297255555620311	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	205	0.11947570563517365	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	204	0.11889289731500208	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	198	0.11539604739397259	No Hit
CTGATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	195	0.11364762243345787	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	186	0.10840234755191366	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	178	0.10373988099054102	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	176	0.10257426435019786	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	173	0.10082583938968313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.828083201715788E-4	0.0
10	0.0	0.0	0.0	5.828083201715788E-4	0.0
11	0.0	0.0	0.0	0.0011656166403431576	0.0
12	0.0	0.0	0.0	0.0011656166403431576	0.0
13	0.0	0.0	0.0	0.0011656166403431576	0.0
14	0.0	0.0	0.0	0.0017484249605147363	0.0
15	0.0	0.0	0.0	0.0017484249605147363	0.0
16	0.0	0.0	0.0	0.002914041600857894	0.0
17	0.0	0.0	0.0	0.002914041600857894	0.0
18	0.0	0.0	0.0	0.002914041600857894	0.0
19	0.0	0.0	0.0	0.002914041600857894	0.0
20	0.0	0.0	0.0	0.0034968499210294725	0.0
21	0.0	0.0	0.0	0.004079658241201051	0.0
22	0.0	0.0	0.0	0.005828083201715788	0.0
23	0.0	0.0	0.0	0.007576508162230524	0.0
24	0.0	0.0	0.0	0.008742124802573682	0.0
25	0.0	0.0	0.0	0.008742124802573682	0.0
26	0.0	0.0	0.0	0.008742124802573682	0.0
27	0.0	0.0	0.0	0.010490549763088417	0.0
28	0.0	0.0	0.0	0.01864986624549052	0.0
29	0.0	0.0	0.0	0.040796582412010514	0.0
30	0.0	0.0	0.0	0.06469172353904525	0.0
31	0.0	0.0	0.0	0.10548830595105575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACA	20	0.0018375745	37.0	1
ACAGACA	35	5.5941564E-7	37.0	17
GCAACGC	25	1.2292524E-4	37.0	3
TAGCAAC	25	1.2292524E-4	37.0	1
CAGACAC	35	5.5941564E-7	37.0	18
AGCAACG	25	1.2292524E-4	37.0	2
TTTACAC	30	8.270801E-6	37.0	2
CGTCTTC	545	0.0	35.302753	37
CTTATAC	860	0.0	35.279068	1
GACAGAC	40	1.5895839E-6	32.375	16
TTATACA	1020	0.0	29.745098	2
ACTGTGC	25	0.0054834792	29.6	8
AGGTGGC	25	0.0054834792	29.6	7
CGGGCAC	25	0.0054834792	29.6	16
GTCTTCT	25	0.0054834792	29.6	37
GGTGGCT	25	0.0054834792	29.6	8
GACACAT	45	3.9821825E-6	28.777777	20
AGACAGA	45	3.9821825E-6	28.777777	15
GGTATCA	280	0.0	28.410715	1
TATACAC	1150	0.0	26.704348	3
>>END_MODULE