FastQCFastQC Report
Fri 10 Feb 2017
ERR1631154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340561
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG186055.4630447996100555No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT172065.052252019462005No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT143444.21187393741503Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC105073.0852035318195568No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC56091.646988351572846Illumina PCR Primer Index 10 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53681.5762227618547044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49541.4546586367787269No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC46861.3759649519469346No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30870.906445541327398No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG24540.7205757558851424No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA20050.5887344704766547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19530.5734655465540681No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17600.5167943481490834No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA16600.4874310329133401No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15290.44896508995451617No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14870.436632497555504No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT14730.4325216334224999No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13910.40844371492919035No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13670.40139651927261194No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13660.40110288612025446No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT12080.35470884804777997No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11900.3494234513053462No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11200.3288691306403258No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9530.27983239419663436No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG9260.2719042990829837No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8720.25604810885568224No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8430.24753274743731665No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7820.2296211251435132No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7740.2272720599246537No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7520.22081213057279017No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7480.21963759796336046No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7310.21464583437328408No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6850.20113870936484213No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6740.19790874468891034No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6670.19585331262240835No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6670.19585331262240835No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6540.19203608164176167No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6420.18851248381347246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.18293345391868124No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6170.18117165500453664No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6080.17852895663331975No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5730.16825179630080955No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5720.16795816314845213No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5520.16208550010130343No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5470.16061733433951625No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5270.15474467129236757No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5180.15210197292115069No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4990.14652294302635946No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4920.1444675109598574No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4910.14417387780749996No Hit
ATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT4890.1435866115027851Illumina PCR Primer Index 10 (95% over 21bp)
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4810.14123754628392565No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4690.13771394845563642No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4540.13330945117027493No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4480.13154765225613033No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4440.1303731196467006No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4380.128611320732556No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4330.1271431549707688No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4220.12391319029483705No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4210.12361955714247962No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4040.11862779355240324No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4010.11774689409533093No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4000.11745326094297351No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3970.11657236148590121No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3960.11627872833354377No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3950.11598509518118633No Hit
TCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT3920.11510419572411403Illumina PCR Primer Index 10 (95% over 24bp)
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3800.11158059789582483No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3770.11069969843875253No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3730.10952516582932278No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3710.10893789952460793No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3710.10893789952460793No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3680.10805700006753563No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3670.10776336691517818No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3650.10717610061046332No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3610.1060015680010336No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA3600.10570793484867616No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3600.10570793484867616No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3580.1051206685439613No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3520.10335886962981669No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3500.10277160332510182No Hit
CTGATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT3470.10189070386802952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3450.10130343756331464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAC251.2318017E-437.01
CGCAGCC200.001840117637.029
CCGCAGC200.001840117637.028
CAGACAC403.819696E-837.018
CTTATAC19950.035.609021
GATACAC651.8189894E-1234.1538473
ACAGACA451.0761687E-732.8888917
CGTCTTC5600.032.70535737
TTATACA22050.032.3854872
GATTACG401.5956921E-632.3751
TGATACA750.032.0666662
TTTACAC352.3834116E-531.7142852
ATTACGG352.3834116E-531.7142852
ATTGTTA352.3834116E-531.71428514
TATTGTT352.3834116E-531.71428513
TCTCGCG352.3834116E-531.71428520
TGTTACG352.3834116E-531.71428516
TTACGGG352.3834116E-531.7142853
AGCAACG303.5942084E-430.8333322
GAGGTCG303.5942084E-430.83333226
Produced by FastQC (version 0.11.5)