FastQCFastQC Report
Fri 10 Feb 2017
ERR1631153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31331
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT384612.27538220931346No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG29139.297500877724937No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT28679.150681433723788RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC15875.065270818039641No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC8592.741693530369283No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC7722.4640132775845007RNA PCR Primer, Index 26 (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4111.3117998148798315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3191.0181609268775336No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG3020.9639015671379784No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2270.7245220388752355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2070.6606874980051706No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT2020.6447288627876544No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1850.5904695030480993RNA PCR Primer, Index 26 (95% over 21bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT810.2585298905237624RNA PCR Primer, Index 26 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC730.2329960741757365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.19150362261019438No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA570.18192844147968465No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA550.17554498739267818No Hit
CTGATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT540.17235326034917495No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA370.11809390060961986No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC330.1053269924356069No Hit
TCGCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT320.10213526539210366RNA PCR Primer, Index 26 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACA200.001814719937.017
ATAAGAG200.001814719937.010
GTGTATA200.001814719937.06
TATAAGA200.001814719937.09
GATGTGT200.001814719937.03
AAGAGAC200.001814719937.012
TAAGAGA200.001814719937.011
ATGTGTA200.001814719937.04
GACACAT200.001814719937.020
AGATGTG200.001814719937.02
CAGACAC200.001814719937.018
TGTGTAT200.001814719937.05
GAGATGT200.001814719937.01
CTTATAC5300.036.3018881
TTATACA5450.035.3027532
TATACAC5650.034.0530973
CGTCTTC1250.034.0437
GGTATCA554.6020432E-1033.6363641
GTATAAG250.005416053329.68
AGAGACA250.005416053329.613
Produced by FastQC (version 0.11.5)