##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631153.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31331
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99004181162427	31.0	30.0	34.0	28.0	34.0
2	31.11081676295043	31.0	31.0	34.0	28.0	34.0
3	30.629887332035363	31.0	30.0	34.0	26.0	34.0
4	34.68954071047844	35.0	35.0	37.0	32.0	37.0
5	34.89566244294788	35.0	35.0	37.0	32.0	37.0
6	35.0683348760014	36.0	35.0	37.0	32.0	37.0
7	35.00995818837573	36.0	35.0	37.0	32.0	37.0
8	35.05263157894737	36.0	35.0	37.0	32.0	37.0
9	36.85803198110498	39.0	37.0	39.0	33.0	39.0
10	36.674794931537456	39.0	35.0	39.0	32.0	39.0
11	36.67833774855574	39.0	35.0	39.0	32.0	39.0
12	36.524496505058885	38.0	35.0	39.0	32.0	39.0
13	36.67393316523571	39.0	35.0	39.0	32.0	39.0
14	37.40001915036226	39.0	36.0	40.0	32.0	41.0
15	37.308703839647634	39.0	36.0	40.0	32.0	41.0
16	37.23226197695573	39.0	36.0	40.0	31.0	41.0
17	37.16274616194823	39.0	36.0	40.0	31.0	41.0
18	37.48536593150554	39.0	36.0	40.0	32.0	41.0
19	37.39462513165874	39.0	36.0	40.0	32.0	41.0
20	37.56318023682615	39.0	37.0	40.0	32.0	41.0
21	37.56662730203313	39.0	37.0	40.0	32.0	41.0
22	37.16919345057611	39.0	36.0	40.0	31.0	41.0
23	37.35236666560276	39.0	36.0	40.0	32.0	41.0
24	36.99016948070601	39.0	36.0	40.0	31.0	41.0
25	36.98033896141202	39.0	36.0	40.0	31.0	41.0
26	36.90277999425489	39.0	35.0	40.0	31.0	41.0
27	36.64702690625898	39.0	35.0	40.0	30.0	41.0
28	36.93517602374645	39.0	35.0	40.0	31.0	41.0
29	36.75353483770068	39.0	35.0	40.0	30.0	41.0
30	36.730107561201365	39.0	35.0	40.0	30.0	41.0
31	36.53739108231464	38.0	35.0	40.0	30.0	41.0
32	36.25249752641154	38.0	35.0	40.0	30.0	41.0
33	35.87801219239731	38.0	35.0	40.0	30.0	41.0
34	35.5654144457566	38.0	34.0	40.0	28.0	41.0
35	35.82388050173949	38.0	35.0	40.0	29.0	41.0
36	35.84271169129616	38.0	35.0	40.0	29.0	41.0
37	35.70336088857681	38.0	34.0	40.0	28.0	41.0
38	35.6737416616131	38.0	35.0	40.0	29.0	41.0
39	35.426255146659855	38.0	34.0	40.0	27.0	41.0
40	34.85879799559542	38.0	33.0	40.0	25.0	41.0
41	34.96007149468578	38.0	33.0	40.0	26.0	41.0
42	34.75988637451725	38.0	33.0	40.0	24.0	41.0
43	33.44984200951134	37.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	7.0
13	3.0
14	1.0
15	3.0
16	0.0
17	6.0
18	4.0
19	12.0
20	15.0
21	18.0
22	30.0
23	74.0
24	105.0
25	157.0
26	259.0
27	385.0
28	474.0
29	671.0
30	942.0
31	1160.0
32	1334.0
33	1622.0
34	2215.0
35	2755.0
36	3559.0
37	4822.0
38	6433.0
39	4259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.602821486706457	25.967891225942356	20.328109540072134	30.101177747279053
2	8.2761482238039	18.524783760492802	46.64070728671284	26.558360728990454
3	9.878395199642528	31.473620375985444	44.74162969582841	13.906354728543615
4	5.907886757524496	25.31039545498069	20.995180492164312	47.786537295330504
5	9.485812773291629	37.438958220293	34.0876448246146	18.987584181800774
6	23.867734831317225	37.74855574351281	14.113816986371324	24.269892438798635
7	15.243688359771474	37.97836009064505	12.814784079665506	33.96316746991797
8	28.068047620567487	30.254380645367206	26.031725766812425	15.64584596725288
9	13.954230634196163	10.369921164342026	11.633845073569308	64.0420031278925
10	12.559445916185247	30.117136382496568	26.571127637164473	30.752290064153716
11	22.862340812613706	15.802240592384539	26.456225463598354	34.879193131403404
12	12.47326928601066	30.812932877980277	15.980977306820721	40.732820529188345
13	34.69726468992372	11.480642175481153	27.097762599342506	26.724330535252626
14	22.195269860521528	27.41693530369283	17.050205866394304	33.337588969391334
15	26.883916887427787	26.555169001946954	13.386103220452586	33.17481089017267
16	37.438958220293	18.89821582458268	14.046790718457757	29.616035236666562
17	13.529730937410234	45.836392071750026	14.618109859244838	26.01576713159491
18	29.408572978838848	12.722223995403914	19.089719447192877	38.77948357856436
19	20.047237560243847	26.207270754205105	26.350898471162747	27.39459321438831
20	14.075516261849286	19.0163097251923	16.660815167086913	50.2473588458715
21	27.61482238039003	14.79684657368102	22.584660559828922	35.00367048610003
22	14.292553700807506	42.35740959433149	27.464811209345374	15.885225495515623
23	31.508729373463986	16.293766557084037	19.1152532635409	33.08225080591108
24	27.091379145255495	33.82592320704733	26.063643037247452	13.019054610449714
25	24.09753917844946	28.498930771440428	18.2247614184035	29.178768631706614
26	25.224218824806105	19.95148574893875	28.645750215441574	26.17854521081357
27	14.870256295681594	36.99849988828955	26.44984200951135	21.681401806517506
28	16.33525900864958	14.905365293160127	28.751077207877184	40.00829849031311
29	11.68491270626536	21.726085985126552	43.448980243209604	23.140021065398486
30	26.245571478727136	13.912738182630621	31.004436500590472	28.83725383805177
31	14.598959496983818	23.835817560882194	19.204621620758992	42.36060132137499
32	30.911876416328877	19.562095049631356	37.831540646643894	11.69448788739587
33	25.106124924196482	17.03424723117679	33.65676167374166	24.202866170885066
34	14.426606236634642	46.37260221505856	21.272860744949092	17.927930803357697
35	26.654112540295554	15.154320002553382	45.035268583830714	13.156298873320354
36	21.314353196514634	17.682167821007948	18.693945293798475	42.30953368867895
37	16.255465832562	27.83185981934825	31.748108901726724	24.164565446363024
38	16.083112572212823	14.720245124636941	36.755928632983306	32.44071367016693
39	30.950177140850915	18.85672337301714	22.064409051737897	28.12869043439405
40	12.38390092879257	14.375538603938592	55.02537422999585	18.215186237272988
41	20.289808815550096	22.951709169831798	20.851552775206663	35.90692923941145
42	24.081580543231944	14.50320768567872	42.734033385464876	18.68117838562446
43	21.521815454342345	14.764929303245985	41.144553317800266	22.568701924611407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	13.5
4	25.0
5	25.0
6	37.5
7	50.0
8	52.0
9	54.0
10	71.5
11	89.0
12	89.0
13	133.5
14	178.0
15	410.0
16	642.0
17	629.0
18	616.0
19	616.0
20	567.5
21	519.0
22	445.5
23	372.0
24	370.0
25	368.0
26	368.0
27	389.0
28	410.0
29	432.0
30	454.0
31	477.5
32	501.0
33	501.0
34	527.0
35	553.0
36	585.0
37	617.0
38	633.0
39	649.0
40	649.0
41	670.0
42	691.0
43	738.0
44	785.0
45	848.0
46	911.0
47	911.0
48	1078.0
49	1245.0
50	1567.5
51	1890.0
52	5084.5
53	8279.0
54	8279.0
55	4998.0
56	1717.0
57	3751.5
58	5786.0
59	4278.5
60	2771.0
61	2771.0
62	1625.5
63	480.0
64	382.0
65	284.0
66	240.0
67	196.0
68	196.0
69	148.5
70	101.0
71	79.5
72	58.0
73	37.0
74	16.0
75	16.0
76	13.0
77	10.0
78	7.5
79	5.0
80	4.5
81	4.0
82	4.0
83	2.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	31331.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.59436341004117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.47039364869336	33.37269796686987
2	7.591796228911678	5.860010851871948
3	2.232881243797552	2.5852989052376243
4	1.1908699966920278	1.838434777057866
5	0.7029440952695998	1.3564839934888768
6	0.4134965266291763	0.957518113050972
7	0.19847833278200464	0.5362101433085442
8	0.19847833278200464	0.612811592352622
9	0.18193847171683758	0.6319619546136415
>10	0.6615944426066821	4.126903067249689
>50	0.04961958319550116	1.2128562765312312
>100	0.05788951372808468	5.914270211611504
>500	0.016539861065167055	5.205706807953784
>1k	0.03307972213033411	35.788835338801825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	3846	12.27538220931346	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	2913	9.297500877724937	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	2867	9.150681433723788	RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	1587	5.065270818039641	No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	859	2.741693530369283	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	772	2.4640132775845007	RNA PCR Primer, Index 26 (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	411	1.3117998148798315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	319	1.0181609268775336	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	302	0.9639015671379784	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	227	0.7245220388752355	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	207	0.6606874980051706	No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT	202	0.6447288627876544	No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	185	0.5904695030480993	RNA PCR Primer, Index 26 (95% over 21bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT	81	0.2585298905237624	RNA PCR Primer, Index 26 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	73	0.2329960741757365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60	0.19150362261019438	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	57	0.18192844147968465	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	55	0.17554498739267818	No Hit
CTGATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	54	0.17235326034917495	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	37	0.11809390060961986	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	33	0.1053269924356069	No Hit
TCGCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	32	0.10213526539210366	RNA PCR Primer, Index 26 (100% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0031917270435032397	0.0	0.0	0.0	0.0
12	0.0031917270435032397	0.0	0.0	0.0	0.0
13	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
14	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
15	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
16	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
17	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
18	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
19	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
20	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
21	0.0031917270435032397	0.0	0.0	0.0031917270435032397	0.0
22	0.0031917270435032397	0.0	0.0	0.00957518113050972	0.0
23	0.0031917270435032397	0.0	0.0	0.012766908174012959	0.0
24	0.0031917270435032397	0.0	0.0	0.012766908174012959	0.0
25	0.0031917270435032397	0.0	0.0	0.012766908174012959	0.0
26	0.0031917270435032397	0.0	0.0	0.01915036226101944	0.0
27	0.0031917270435032397	0.0	0.0	0.01915036226101944	0.0
28	0.0031917270435032397	0.0	0.0	0.0319172704350324	0.0
29	0.0031917270435032397	0.0	0.0	0.07660144904407776	0.0
30	0.0031917270435032397	0.0	0.0	0.11490217356611662	0.0
31	0.0031917270435032397	0.0	0.0	0.17873671443618142	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACA	20	0.0018147199	37.0	17
ATAAGAG	20	0.0018147199	37.0	10
GTGTATA	20	0.0018147199	37.0	6
TATAAGA	20	0.0018147199	37.0	9
GATGTGT	20	0.0018147199	37.0	3
AAGAGAC	20	0.0018147199	37.0	12
TAAGAGA	20	0.0018147199	37.0	11
ATGTGTA	20	0.0018147199	37.0	4
GACACAT	20	0.0018147199	37.0	20
AGATGTG	20	0.0018147199	37.0	2
CAGACAC	20	0.0018147199	37.0	18
TGTGTAT	20	0.0018147199	37.0	5
GAGATGT	20	0.0018147199	37.0	1
CTTATAC	530	0.0	36.301888	1
TTATACA	545	0.0	35.302753	2
TATACAC	565	0.0	34.053097	3
CGTCTTC	125	0.0	34.04	37
GGTATCA	55	4.6020432E-10	33.636364	1
GTATAAG	25	0.0054160533	29.6	8
AGAGACA	25	0.0054160533	29.6	13
>>END_MODULE