FastQCFastQC Report
Fri 10 Feb 2017
ERR1631152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577298
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG191403.3154454025477307No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT137572.382998035676548TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC104411.8085979857889685No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT103951.8006298306940263No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC52490.9092357846380899RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC48150.8340579735249386No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG25650.4443112569245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.4287213882604825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24300.42092645392847367No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA16160.2799247529005817No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT15320.2653742088141653No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11350.19660556592955455No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10650.18448011252420762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9490.16438650402391833No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7760.13441931203641794No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7240.1254118323638745No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6880.11917588489826746No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6860.11882944337240038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGA200.00184117737.026
CTTATAC16200.034.3734551
CGTCTTC8050.032.17391237
TTATACA18350.030.346052
GATTACG405.9350852E-527.7500021
GGTATCA4750.027.2631571
TATACAC21450.026.6503493
CGAACGC509.0808535E-625.90000230
ACGAACG451.3220974E-424.66666829
GTATCAA12650.023.5454561
CGGAAAT551.900532E-523.54545437
AGCAACG551.900532E-523.5454542
TTCAACG551.900532E-523.54545419
CGTTTGT551.900532E-523.54545413
TACGTGA551.900532E-523.54545436
CCGTCTT21800.023.5068837
GCCGTCT22150.023.1354436
CTCCACG502.6998445E-422.211
CTACGAA502.6998445E-422.227
TACGAAC502.6998445E-422.228
Produced by FastQC (version 0.11.5)