##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577298
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19500154166479	31.0	30.0	31.0	26.0	34.0
2	30.168141930164317	31.0	30.0	33.0	26.0	34.0
3	29.611271821485612	31.0	30.0	33.0	25.0	34.0
4	33.851182578148546	35.0	33.0	37.0	28.0	37.0
5	33.494612834272765	35.0	33.0	37.0	28.0	37.0
6	33.801201459211704	35.0	33.0	37.0	29.0	37.0
7	33.90113251734806	35.0	33.0	37.0	30.0	37.0
8	33.95134575210723	35.0	33.0	37.0	30.0	37.0
9	35.54052153307304	37.0	35.0	39.0	30.0	39.0
10	35.16065186437507	37.0	34.0	39.0	29.0	39.0
11	35.37171616738669	37.0	34.0	39.0	30.0	39.0
12	35.225152347661	37.0	34.0	39.0	30.0	39.0
13	35.423866010275454	37.0	34.0	39.0	30.0	39.0
14	36.13155250840987	38.0	34.0	40.0	30.0	41.0
15	36.138954231610015	38.0	34.0	40.0	30.0	41.0
16	36.15987583535713	38.0	35.0	40.0	30.0	41.0
17	36.03675224927161	38.0	34.0	40.0	30.0	41.0
18	36.17957623272556	38.0	35.0	40.0	30.0	41.0
19	36.174197728036475	38.0	34.0	40.0	30.0	41.0
20	36.172023807461656	38.0	34.0	40.0	30.0	41.0
21	36.074228561332276	38.0	34.0	40.0	30.0	41.0
22	35.777456357028775	38.0	34.0	40.0	29.0	41.0
23	35.86958208758735	38.0	34.0	40.0	29.0	41.0
24	35.71364009575644	38.0	34.0	40.0	29.0	41.0
25	35.608592096283026	38.0	34.0	40.0	28.0	41.0
26	35.47310574434694	38.0	34.0	40.0	27.0	41.0
27	35.23826862383033	38.0	34.0	40.0	27.0	40.0
28	35.23974273252289	38.0	33.0	40.0	27.0	40.0
29	35.015884343961005	37.0	33.0	39.0	27.0	40.0
30	34.86904163880699	37.0	33.0	39.0	27.0	40.0
31	34.88637757276138	37.0	33.0	39.0	27.0	40.0
32	34.47662905466501	37.0	33.0	39.0	25.0	40.0
33	34.51943536960114	37.0	33.0	39.0	25.0	40.0
34	34.455631233782206	37.0	33.0	39.0	25.0	40.0
35	34.29850614414046	37.0	32.0	39.0	25.0	40.0
36	34.43943855686318	37.0	33.0	39.0	25.0	40.0
37	34.4467502052666	37.0	33.0	39.0	25.0	40.0
38	34.36437333924594	37.0	33.0	39.0	25.0	40.0
39	34.232877993687836	37.0	32.0	39.0	25.0	40.0
40	33.99570758949451	37.0	32.0	39.0	24.0	40.0
41	33.98304515172406	37.0	32.0	39.0	24.0	40.0
42	33.89404259152119	36.0	32.0	39.0	24.0	40.0
43	32.94994786055036	36.0	30.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	13.0
10	20.0
11	21.0
12	23.0
13	17.0
14	15.0
15	19.0
16	23.0
17	40.0
18	78.0
19	165.0
20	334.0
21	611.0
22	1054.0
23	1946.0
24	3066.0
25	4510.0
26	6879.0
27	9919.0
28	13609.0
29	18256.0
30	23982.0
31	30441.0
32	38244.0
33	48596.0
34	60419.0
35	72952.0
36	83557.0
37	81458.0
38	59626.0
39	17403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.25128789637241	25.88922878651927	16.662105186576085	23.197378130532236
2	14.397416931983134	23.143160031734045	39.9895721100714	22.469850926211418
3	15.611867700910102	30.88335660265582	33.96807194897609	19.536703747457985
4	11.075562361206865	20.339755204417823	36.20469843997381	32.379983994401506
5	11.473104012139311	39.976060890562586	34.40164351859871	14.149191578699389
6	25.122726910538407	40.82709449885501	17.556617206364823	16.49356138424176
7	22.104355116421676	35.27606192988716	21.78753434101625	20.83204861267491
8	24.669061732415496	33.43316623303736	24.137447210972496	17.760324823574653
9	22.13744028214198	14.049762860775544	19.032977768847285	44.77981908823519
10	13.998316294184285	27.684142332036487	33.896185332358684	24.42135604142055
11	30.073029873652775	22.58885359034675	24.385499343493308	22.952617192507162
12	18.87309500465964	28.4942958402766	27.130875215226798	25.501733939836964
13	30.831736815301632	19.714947912516585	25.576565309424247	23.876749962757536
14	21.121500507536837	24.840030625430888	25.72969246385749	28.30877640317479
15	25.024510737955097	28.881271024670102	21.727426736278318	24.36679150109649
16	24.335784984531386	27.20570658481408	22.554382658522982	25.904125772131554
17	19.07940093331347	32.29458615827527	23.815256591916135	24.81075631649512
18	23.91243343992184	23.998350938336873	24.912090462811236	27.17712515893005
19	25.508143108065507	28.064881568964385	24.832408911861812	21.5945664111083
20	22.11145716770195	25.47055420250893	24.640999968820264	27.77698866096886
21	28.81735256314763	24.765199255843605	25.510048536457774	20.907399644550996
22	19.246385748781393	32.91783446331011	27.779760193175797	20.056019594732703
23	25.273602195053506	26.517673714442108	25.33007216376984	22.87865192673455
24	25.8557971792731	27.96943692858801	26.476273952100993	19.6984919400379
25	20.193037218213124	28.56964687215268	27.639278154436703	23.598037755197492
26	27.62195607814335	28.50434264452674	25.135198805469617	18.73850247186029
27	19.236338944531248	29.494818966980656	28.48251682839712	22.786325260090976
28	23.00631562901656	26.984503670547966	28.081683982968936	21.927496717466543
29	23.858042120360714	24.9093189306043	29.73109208762199	21.501546861412997
30	19.490973466043535	28.320382194291337	30.4750752644215	21.713569075243637
31	27.129143007597463	26.182145096639864	24.887146672948806	21.801565222813867
32	21.098981808355475	26.367144871452876	33.17870493228801	19.35516838790365
33	18.97477559250162	30.979320905320996	30.29024871037142	19.755654791805966
34	21.9494957543591	29.9781048955652	26.91850655987029	21.153892790205404
35	19.820959019431903	25.220769862358782	36.10353751442063	18.854733603788684
36	22.23531001319942	27.84835561529747	24.802614940637245	25.11371943086586
37	21.45079317787347	25.803830950393035	29.90084843529685	22.84452743643664
38	20.998686986616963	24.051529712557464	29.09381290078954	25.855970400036032
39	25.18144874917287	25.239131263229737	27.300978004427524	22.27844198316987
40	19.67198916330907	23.826515941506813	35.2441893095074	21.257305585676722
41	22.4516627461034	26.4509837207127	26.13537549064781	24.96197804253609
42	20.902722683951787	24.215569775055517	33.01362554521235	21.868081995780344
43	23.330238455702254	23.380818918478845	30.368198053691508	22.920744572127393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	12.0
2	12.0
3	52.0
4	92.0
5	92.0
6	122.0
7	152.0
8	185.0
9	218.0
10	327.0
11	436.0
12	436.0
13	729.0
14	1022.0
15	2476.5
16	3931.0
17	4375.0
18	4819.0
19	4819.0
20	4906.0
21	4993.0
22	5845.0
23	6697.0
24	8730.0
25	10763.0
26	10763.0
27	12609.0
28	14455.0
29	16745.5
30	19036.0
31	22107.0
32	25178.0
33	25178.0
34	27547.5
35	29917.0
36	32045.0
37	34173.0
38	35006.5
39	35840.0
40	35840.0
41	36844.5
42	37849.0
43	38508.0
44	39167.0
45	39096.0
46	39025.0
47	39025.0
48	40587.5
49	42150.0
50	54168.5
51	66187.0
52	50469.5
53	34752.0
54	34752.0
55	44871.0
56	54990.0
57	45017.0
58	35044.0
59	24876.5
60	14709.0
61	14709.0
62	12093.5
63	9478.0
64	7514.5
65	5551.0
66	4331.5
67	3112.0
68	3112.0
69	2486.5
70	1861.0
71	1379.5
72	898.0
73	632.5
74	367.0
75	367.0
76	262.5
77	158.0
78	120.0
79	82.0
80	67.5
81	53.0
82	53.0
83	47.5
84	42.0
85	37.0
86	32.0
87	29.0
88	26.0
89	26.0
90	21.0
91	16.0
92	9.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	577298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.06189793542638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.16320415149158	39.608801236959515
2	9.020522242398478	8.490457941920013
3	2.3775663260199713	3.3567835130957575
4	0.9751974296259097	1.8357856759977889
5	0.5907034094156539	1.3899811782013942
6	0.4058727753719629	1.1460685877594137
7	0.28172777847042707	0.9281050769164757
8	0.21623444566519476	0.8141122729615119
9	0.19525283533482193	0.8270072107317004
>10	1.4979784417089217	14.19560551874718
>50	0.1768413588697901	5.775046737724635
>100	0.09154573457691598	7.530382718693275
>500	0.0025735748676241443	0.8455719026297973
>1k	0.002941228420141879	3.0509452341050167
>5k	3.6765355251773486E-4	0.9082068583801527
>10k+	0.0014706142100709395	9.297138335176365
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	19140	3.3154454025477307	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	13757	2.382998035676548	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	10441	1.8085979857889685	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	10395	1.8006298306940263	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	5249	0.9092357846380899	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	4815	0.8340579735249386	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	2565	0.4443112569245	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2475	0.4287213882604825	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2430	0.42092645392847367	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1616	0.2799247529005817	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	1532	0.2653742088141653	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	1135	0.19660556592955455	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1065	0.18448011252420762	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	949	0.16438650402391833	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	776	0.13441931203641794	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	724	0.1254118323638745	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	688	0.11917588489826746	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	686	0.11882944337240038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7322076293352825E-4	0.0
4	0.0	0.0	0.0	3.464415258670565E-4	0.0
5	0.0	0.0	0.0	3.464415258670565E-4	0.0
6	0.0	0.0	0.0	3.464415258670565E-4	0.0
7	0.0	0.0	0.0	3.464415258670565E-4	0.0
8	0.0	0.0	0.0	3.464415258670565E-4	0.0
9	0.0	0.0	0.0	5.196622888005848E-4	0.0
10	0.0	0.0	0.0	6.92883051734113E-4	0.0
11	0.0	0.0	0.0	6.92883051734113E-4	0.0
12	0.0	0.0	0.0	6.92883051734113E-4	0.0
13	0.0	0.0	0.0	6.92883051734113E-4	0.0
14	0.0	0.0	0.0	6.92883051734113E-4	0.0
15	0.0	0.0	0.0	8.661038146676413E-4	0.0
16	0.0	0.0	0.0	8.661038146676413E-4	0.0
17	0.0	0.0	0.0	8.661038146676413E-4	0.0
18	0.0	0.0	0.0	8.661038146676413E-4	0.0
19	0.0	0.0	0.0	8.661038146676413E-4	0.0
20	0.0	0.0	0.0	8.661038146676413E-4	0.0
21	0.0	0.0	0.0	0.0017322076293352826	0.0
22	0.0	0.0	0.0	0.001905428392268811	0.0
23	0.0	0.0	0.0	0.002598311444002924	0.0
24	0.0	0.0	0.0	0.003291194495737037	0.0
25	0.0	0.0	0.0	0.003291194495737037	0.0
26	0.0	0.0	0.0	0.0036376360216040936	0.0
27	0.0	0.0	0.0	0.0048501813621387915	0.0
28	0.0	0.0	0.0	0.009700362724277583	0.0
29	0.0	0.0	0.0	0.01818818010802047	0.0
30	0.0	0.0	0.0	0.032219061905636254	0.0
31	0.0	0.0	0.0	0.06980796746221189	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCGA	20	0.001841177	37.0	26
CTTATAC	1620	0.0	34.373455	1
CGTCTTC	805	0.0	32.173912	37
TTATACA	1835	0.0	30.34605	2
GATTACG	40	5.9350852E-5	27.750002	1
GGTATCA	475	0.0	27.263157	1
TATACAC	2145	0.0	26.650349	3
CGAACGC	50	9.0808535E-6	25.900002	30
ACGAACG	45	1.3220974E-4	24.666668	29
GTATCAA	1265	0.0	23.545456	1
CGGAAAT	55	1.900532E-5	23.545454	37
AGCAACG	55	1.900532E-5	23.545454	2
TTCAACG	55	1.900532E-5	23.545454	19
CGTTTGT	55	1.900532E-5	23.545454	13
TACGTGA	55	1.900532E-5	23.545454	36
CCGTCTT	2180	0.0	23.50688	37
GCCGTCT	2215	0.0	23.13544	36
CTCCACG	50	2.6998445E-4	22.2	11
CTACGAA	50	2.6998445E-4	22.2	27
TACGAAC	50	2.6998445E-4	22.2	28
>>END_MODULE