FastQCFastQC Report
Fri 10 Feb 2017
ERR1631151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27437
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT403514.706418340197544No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG313611.429821044574844No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT306611.174691110544156RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC17006.196012683602435No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC8473.087072201771331No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC8152.970441374785873RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4021.465174764004811No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG3641.3266756569595801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3121.1371505631082115No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA2460.8965994824507053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2380.8674417757043409No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT2040.7435215220322922No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1840.6706272551663812No Hit
CTGATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT760.27699821409046177No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA750.27335350074716624No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT720.2624193607172796No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC670.24419579400080182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.2223275139410285No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA480.17494624047818638No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA440.1603673871050042TruSeq Adapter, Index 25 (96% over 27bp)
TCGCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT420.1530779604184131RNA PCR Primer, Index 17 (95% over 21bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT400.145788533731822No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA360.1312096803586398No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG310.11298611364216206No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT310.11298611364216206No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG290.10569668695557094No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC290.10569668695557094No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG280.1020519736122754No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT280.1020519736122754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA251.20245924E-437.025
GCTTGAA251.20245924E-437.026
CTGCTTG251.20245924E-437.024
TTGAAAA251.20245924E-437.028
CTTGAAA251.20245924E-437.027
CTTATAC4850.036.2371141
TTATACA5000.035.1499982
TATACAC5350.033.1962623
TCTGCTT303.5093876E-430.83333223
TGAAAAA303.5093876E-430.83333229
TTCTGCT358.6494756E-426.4285722
TCTTCTG358.6494756E-426.4285720
CTTCTGC358.6494756E-426.4285721
CCGTCTT4800.025.82291637
GCCGTCT5000.024.78999936
GGTATCA901.2551027E-1024.6666661
CGTCTTC1450.024.24137937
TACACAT8900.020.7865185
ATACACA8750.020.508574
TGCCGTC6100.020.31967235
Produced by FastQC (version 0.11.5)